Safety Evaluations on Ethanolic Extract of Red Cabbage (Brassica oleracea L.) in Mice

Menaka Thounaojam, Ravirajsinh N. Jadeja, Jayanta M. Sankhari, Ranjitsinh V. Devkar, A. V. Ramachandran

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The present study has carried out safety evaluations on an ethanolic extract of red cabbage (RC) leaves in terms of acute and subchronic oral toxicity tests as per Organisation for Economic Cooperation and Development (OECD) guidelines in Swiss albino mice. Single-dose administration of RC extract (1000, 2000, 3000, 4000, or 5000 mg/kg body weight) to Swiss albino mice did not manifest toxicity or any significant adverse behavioral alterations. Chronic administration of RC extract (1000, 2000, and 3000 mg/kg body weight) for 28 d also did not register any significant alterations in fluid intake, organ weights, plasma lipid profile, plasma creatine kinase-MB, lactate dehydrogenase, aspartate transaminase, alanine transaminase, creatinine, electrolytes, and calcium levels, and the total blood count showed a nonsignificant change. However, significant reduction in body-weight gain, food intake, red blood cell count, and hemoglobin content along with higher alkaline phosphatase, bilirubin, and urea levels was observed in mice treated with 3000 mg/kg body weight for 28 d. Since there was no mortality up to a dose of 5000 mg/kg body weight, 50% lethal dose (LD50) could not be determined, and hence, it can be assumed that, LD50 of RC extract is >5000 mg/kg. No observable adverse effect level dose of the RC extract was found to be 2000 mg/kg body weight. Hence, consumption of RC extract for various medicinal purposes is safe.

Original languageEnglish (US)
JournalJournal of Food Science
Volume76
Issue number1
DOIs
StatePublished - Jan 1 2011

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Brassica
Brassica oleracea
cabbage
Body Weight
Safety
body weight
Lethal Dose 50
mice
extracts
lethal dose 50
albino
Subchronic Toxicity Tests
dosage
drug toxicity
MB Form Creatine Kinase
Erythrocyte Count
Organ Size
bilirubin
erythrocyte count
creatine kinase

Keywords

  • Cabbage
  • Safety
  • Toxicity

ASJC Scopus subject areas

  • Food Science

Cite this

Safety Evaluations on Ethanolic Extract of Red Cabbage (Brassica oleracea L.) in Mice. / Thounaojam, Menaka; Jadeja, Ravirajsinh N.; Sankhari, Jayanta M.; Devkar, Ranjitsinh V.; Ramachandran, A. V.

In: Journal of Food Science, Vol. 76, No. 1, 01.01.2011.

Research output: Contribution to journalArticle

Thounaojam, Menaka ; Jadeja, Ravirajsinh N. ; Sankhari, Jayanta M. ; Devkar, Ranjitsinh V. ; Ramachandran, A. V. / Safety Evaluations on Ethanolic Extract of Red Cabbage (Brassica oleracea L.) in Mice. In: Journal of Food Science. 2011 ; Vol. 76, No. 1.
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