Salicylate reduces cisplatin nephrotoxicity by inhibition of tumor necrosis factor-α

Ganesan Ramesh, W. Brian Reeves

Research output: Contribution to journalArticle

140 Citations (Scopus)

Abstract

Background. Salicylate was recently shown to provide protection against cisplatin nephrotoxicity in rats. We have demonstrated that enhanced tumor necrosis factor-α (TNF-α) production mediates, in part, cisplatin nephrotoxicity. The purpose of this study was to determine if the protective effects of salicylate were mediated through inhibition of TNF-α in vivo and to explore the mechanism of inhibition in vitro. Methods. The effects of treatment with cisplatin alone and in combination with sodium salicylate in mice on renal function, histology, and gene expression were determined. The effects of cisplatin and salicylate on TNF-α expression, nuclear factor kappa B (NF-κB) activity, and apoptosis were determined in vitro using cultured murine proximal tubule cells. Results. Salicylate significantly reduced both the functional and histologic evidence of cisplatin renal injury. Cisplatin increased the renal expression of TNF-α, monocyte chemoattractant protein-1 (MCP-1), intercellular adhesion molecule (ICAM)-1, heme oxygenase-1, TNF receptor 1 (TNFR1), and TNF receptor 2 (TNFR2). Treatment with sodium salicylate blunted the increase in TNF-α mRNA and also reduced serum TNF-α protein levels. Salicylate had little protective effect when administered with cisplatin to TNF-α-deficient mice. Cisplatin increased the degradation of I kappa B (IκB) in a time-dependent manner and also increased nuclear NF-κB binding activity. Salicylate inhibited IκB degradation and NF-κB binding activity in the presence of cisplatin. In addition, salicylate inhibited the cisplatin induced TNF-α mRNA increase in mouse proximal tubule epithelial (TKPT) cells. Conclusion. These results indicate that salicylate acts via inhibition of TNF-α production to reduce cisplatin nephrotoxicity. The inhibition of TNF-α production may be mediated via stabilization of IκB.

Original languageEnglish (US)
Pages (from-to)490-498
Number of pages9
JournalKidney International
Volume65
Issue number2
DOIs
StatePublished - Jan 1 2004

Fingerprint

Salicylates
Cisplatin
Tumor Necrosis Factor-alpha
I-kappa B Proteins
NF-kappa B
Sodium Salicylate
Tumor Necrosis Factor Receptors
Kidney
Messenger RNA
Heme Oxygenase-1
Chemokine CCL2
Intercellular Adhesion Molecule-1
Histology
Epithelial Cells
Apoptosis
Gene Expression

Keywords

  • Acute tubular necrosis
  • Cytokines
  • Gene expression
  • IκB
  • NF-κB
  • Tumor necrosis factor receptor

ASJC Scopus subject areas

  • Nephrology

Cite this

Salicylate reduces cisplatin nephrotoxicity by inhibition of tumor necrosis factor-α. / Ramesh, Ganesan; Reeves, W. Brian.

In: Kidney International, Vol. 65, No. 2, 01.01.2004, p. 490-498.

Research output: Contribution to journalArticle

Ramesh, Ganesan ; Reeves, W. Brian. / Salicylate reduces cisplatin nephrotoxicity by inhibition of tumor necrosis factor-α. In: Kidney International. 2004 ; Vol. 65, No. 2. pp. 490-498.
@article{d51e731089b940f7888e1543883e5f53,
title = "Salicylate reduces cisplatin nephrotoxicity by inhibition of tumor necrosis factor-α",
abstract = "Background. Salicylate was recently shown to provide protection against cisplatin nephrotoxicity in rats. We have demonstrated that enhanced tumor necrosis factor-α (TNF-α) production mediates, in part, cisplatin nephrotoxicity. The purpose of this study was to determine if the protective effects of salicylate were mediated through inhibition of TNF-α in vivo and to explore the mechanism of inhibition in vitro. Methods. The effects of treatment with cisplatin alone and in combination with sodium salicylate in mice on renal function, histology, and gene expression were determined. The effects of cisplatin and salicylate on TNF-α expression, nuclear factor kappa B (NF-κB) activity, and apoptosis were determined in vitro using cultured murine proximal tubule cells. Results. Salicylate significantly reduced both the functional and histologic evidence of cisplatin renal injury. Cisplatin increased the renal expression of TNF-α, monocyte chemoattractant protein-1 (MCP-1), intercellular adhesion molecule (ICAM)-1, heme oxygenase-1, TNF receptor 1 (TNFR1), and TNF receptor 2 (TNFR2). Treatment with sodium salicylate blunted the increase in TNF-α mRNA and also reduced serum TNF-α protein levels. Salicylate had little protective effect when administered with cisplatin to TNF-α-deficient mice. Cisplatin increased the degradation of I kappa B (IκB) in a time-dependent manner and also increased nuclear NF-κB binding activity. Salicylate inhibited IκB degradation and NF-κB binding activity in the presence of cisplatin. In addition, salicylate inhibited the cisplatin induced TNF-α mRNA increase in mouse proximal tubule epithelial (TKPT) cells. Conclusion. These results indicate that salicylate acts via inhibition of TNF-α production to reduce cisplatin nephrotoxicity. The inhibition of TNF-α production may be mediated via stabilization of IκB.",
keywords = "Acute tubular necrosis, Cytokines, Gene expression, IκB, NF-κB, Tumor necrosis factor receptor",
author = "Ganesan Ramesh and Reeves, {W. Brian}",
year = "2004",
month = "1",
day = "1",
doi = "10.1111/j.1523-1755.2004.00413.x",
language = "English (US)",
volume = "65",
pages = "490--498",
journal = "Kidney International",
issn = "0085-2538",
publisher = "Nature Publishing Group",
number = "2",

}

TY - JOUR

T1 - Salicylate reduces cisplatin nephrotoxicity by inhibition of tumor necrosis factor-α

AU - Ramesh, Ganesan

AU - Reeves, W. Brian

PY - 2004/1/1

Y1 - 2004/1/1

N2 - Background. Salicylate was recently shown to provide protection against cisplatin nephrotoxicity in rats. We have demonstrated that enhanced tumor necrosis factor-α (TNF-α) production mediates, in part, cisplatin nephrotoxicity. The purpose of this study was to determine if the protective effects of salicylate were mediated through inhibition of TNF-α in vivo and to explore the mechanism of inhibition in vitro. Methods. The effects of treatment with cisplatin alone and in combination with sodium salicylate in mice on renal function, histology, and gene expression were determined. The effects of cisplatin and salicylate on TNF-α expression, nuclear factor kappa B (NF-κB) activity, and apoptosis were determined in vitro using cultured murine proximal tubule cells. Results. Salicylate significantly reduced both the functional and histologic evidence of cisplatin renal injury. Cisplatin increased the renal expression of TNF-α, monocyte chemoattractant protein-1 (MCP-1), intercellular adhesion molecule (ICAM)-1, heme oxygenase-1, TNF receptor 1 (TNFR1), and TNF receptor 2 (TNFR2). Treatment with sodium salicylate blunted the increase in TNF-α mRNA and also reduced serum TNF-α protein levels. Salicylate had little protective effect when administered with cisplatin to TNF-α-deficient mice. Cisplatin increased the degradation of I kappa B (IκB) in a time-dependent manner and also increased nuclear NF-κB binding activity. Salicylate inhibited IκB degradation and NF-κB binding activity in the presence of cisplatin. In addition, salicylate inhibited the cisplatin induced TNF-α mRNA increase in mouse proximal tubule epithelial (TKPT) cells. Conclusion. These results indicate that salicylate acts via inhibition of TNF-α production to reduce cisplatin nephrotoxicity. The inhibition of TNF-α production may be mediated via stabilization of IκB.

AB - Background. Salicylate was recently shown to provide protection against cisplatin nephrotoxicity in rats. We have demonstrated that enhanced tumor necrosis factor-α (TNF-α) production mediates, in part, cisplatin nephrotoxicity. The purpose of this study was to determine if the protective effects of salicylate were mediated through inhibition of TNF-α in vivo and to explore the mechanism of inhibition in vitro. Methods. The effects of treatment with cisplatin alone and in combination with sodium salicylate in mice on renal function, histology, and gene expression were determined. The effects of cisplatin and salicylate on TNF-α expression, nuclear factor kappa B (NF-κB) activity, and apoptosis were determined in vitro using cultured murine proximal tubule cells. Results. Salicylate significantly reduced both the functional and histologic evidence of cisplatin renal injury. Cisplatin increased the renal expression of TNF-α, monocyte chemoattractant protein-1 (MCP-1), intercellular adhesion molecule (ICAM)-1, heme oxygenase-1, TNF receptor 1 (TNFR1), and TNF receptor 2 (TNFR2). Treatment with sodium salicylate blunted the increase in TNF-α mRNA and also reduced serum TNF-α protein levels. Salicylate had little protective effect when administered with cisplatin to TNF-α-deficient mice. Cisplatin increased the degradation of I kappa B (IκB) in a time-dependent manner and also increased nuclear NF-κB binding activity. Salicylate inhibited IκB degradation and NF-κB binding activity in the presence of cisplatin. In addition, salicylate inhibited the cisplatin induced TNF-α mRNA increase in mouse proximal tubule epithelial (TKPT) cells. Conclusion. These results indicate that salicylate acts via inhibition of TNF-α production to reduce cisplatin nephrotoxicity. The inhibition of TNF-α production may be mediated via stabilization of IκB.

KW - Acute tubular necrosis

KW - Cytokines

KW - Gene expression

KW - IκB

KW - NF-κB

KW - Tumor necrosis factor receptor

UR - http://www.scopus.com/inward/record.url?scp=1642480062&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=1642480062&partnerID=8YFLogxK

U2 - 10.1111/j.1523-1755.2004.00413.x

DO - 10.1111/j.1523-1755.2004.00413.x

M3 - Article

VL - 65

SP - 490

EP - 498

JO - Kidney International

JF - Kidney International

SN - 0085-2538

IS - 2

ER -