Salivary markers and risk factor data: A multivariate modeling approach for head and neck squamous cell carcinoma detection

Lutécia H.Mateus Pereira, Islamiyat Nancy Adebisi, Aymee Perez, Michael Wiebel, Isildinha Reis, Robert Duncan, W. Jarrard Goodwin, Jennifer J. Hu, Vinata B Lokeshwar, Elizabeth J. Franzmann

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Background: Head and neck squamous cell carcinoma (HNSCC) is a debilitating and deadly disease largely due to late stage diagnosis. Prior work indicates that soluble CD44 (solCD44) and total protein may be useful diagnostic markers for HNSCC. In this study we combine the markers solCD44, IL-8, HA, and total protein with demographic and risk factor data to derive a multivariate logistic model that improves HNSCC detection as compared to our previous data using biomarkers alone. Methods: We performed the solCD44, IL-8, HA, and total protein assays on oral rinses from 40 HNSCC patients and 39 controls using ELISA assays. Controls had benign diseases of the upper aerodigestive tract and a history of tobacco or alcohol use. All subjects completed a questionnaire including demographic and risk factor data. Results: Depending on cancer subsite, differences between cases and controls were found for all markers. A multivariate logistic model including solCD44, total protein and variables related to smoking, oral health and education offered a significant improvement over the univariate models with an AUC of 0.853. Sensitivity ranged from 75-82.5% and specificity from 69.2-82.1% depending on predictive probability cut points. Conclusion: A multivariate model, including simple and inexpensive molecular tests in combination with risk factors, results in a promising tool for distinguishing HNSCC patients from controls. Impact: In this case-control study, the resulting observations led to an unprecedented multivariate model that distinguished HNSCC cases from controls with better accuracy than the current gold standard which includes oral examination followed by tissue biopsy. Since the components are simple, noninvasive, and inexpensive to obtain, this model combining biomarkers, risk factor and demographic data serves as a promising prototype for future cancer detection tests.

Original languageEnglish (US)
Pages (from-to)241-249
Number of pages9
JournalCancer Biomarkers
Volume10
Issue number5
DOIs
StatePublished - Dec 1 2011

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Demography
Interleukin-8
Proteins
Biomarkers
Logistic Models
Oral Diagnosis
Delayed Diagnosis
Oral Health
Health Education
Area Under Curve
Tobacco
Carcinoma, squamous cell of head and neck
Case-Control Studies
Neoplasms
Smoking
Enzyme-Linked Immunosorbent Assay
Alcohols
Biopsy
Surveys and Questionnaires

Keywords

  • CD44
  • IL-8
  • head and neck cancer
  • hyaluronic acid
  • protein

ASJC Scopus subject areas

  • Oncology
  • Genetics
  • Cancer Research

Cite this

Pereira, L. H. M., Adebisi, I. N., Perez, A., Wiebel, M., Reis, I., Duncan, R., ... Franzmann, E. J. (2011). Salivary markers and risk factor data: A multivariate modeling approach for head and neck squamous cell carcinoma detection. Cancer Biomarkers, 10(5), 241-249. https://doi.org/10.3233/CBM-2012-0252

Salivary markers and risk factor data : A multivariate modeling approach for head and neck squamous cell carcinoma detection. / Pereira, Lutécia H.Mateus; Adebisi, Islamiyat Nancy; Perez, Aymee; Wiebel, Michael; Reis, Isildinha; Duncan, Robert; Goodwin, W. Jarrard; Hu, Jennifer J.; Lokeshwar, Vinata B; Franzmann, Elizabeth J.

In: Cancer Biomarkers, Vol. 10, No. 5, 01.12.2011, p. 241-249.

Research output: Contribution to journalArticle

Pereira, LHM, Adebisi, IN, Perez, A, Wiebel, M, Reis, I, Duncan, R, Goodwin, WJ, Hu, JJ, Lokeshwar, VB & Franzmann, EJ 2011, 'Salivary markers and risk factor data: A multivariate modeling approach for head and neck squamous cell carcinoma detection', Cancer Biomarkers, vol. 10, no. 5, pp. 241-249. https://doi.org/10.3233/CBM-2012-0252
Pereira, Lutécia H.Mateus ; Adebisi, Islamiyat Nancy ; Perez, Aymee ; Wiebel, Michael ; Reis, Isildinha ; Duncan, Robert ; Goodwin, W. Jarrard ; Hu, Jennifer J. ; Lokeshwar, Vinata B ; Franzmann, Elizabeth J. / Salivary markers and risk factor data : A multivariate modeling approach for head and neck squamous cell carcinoma detection. In: Cancer Biomarkers. 2011 ; Vol. 10, No. 5. pp. 241-249.
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