SAMP32, a testis-specific, isoantigenic sperm acrosomal membrane-associated protein

Zhonglin Hao, Michael J. Wolkowicz, Jagathpala Shetty, Kenneth Klotz, Laura Bolling, Buer Sen, V. Anne Westbrook, Scott Coonrod, Charles J. Flickinger, John C. Herr

Research output: Contribution to journalArticle

79 Citations (Scopus)

Abstract

To identify novel human sperm membrane antigens, we analyzed two-dimensional gels of sperm extracts containing hydrophobic proteins that partitioned into Triton X-114. Four protein spots with isoelectric points (pls) ranging from 4.5 to 5.5 and apparent molecular weights from 32 to 34 kDa were sequenced by mass spectrometry and found to contain common peptide sequences. Cloning the corresponding cDNA revealed that these protein spots were products of a single gene (SAMP32), encoding a protein of 32 kDa with a predicted pl of 4.57. SAMP32 has a potential transmembrane domain in the carboxyl terminus and is phosphorylated in vivo on serine 256. Northern blotting of eight human tissues and RNA dot blotting of 76 human tissues showed that SAMP32 expression was testis specific. SAMP32 contained an amino terminal domain homologous to the major malarial circumsporozoite surface protein and a domain similar to that of Krp1 from Schizosaccharomyces pombe in its carboxyl terminus. The SAMP32 locus consists of seven exons on chromosome 6q15-16.2. Antiserum against recombinant SAMP32 recognized protein spots originally cored from a two-dimensional gel. This antiserum strongly stained the equatorial segment and faintly stained the acrosome cap of ejaculated human spermatozoa by immunofluorescence. Immunoelectron microscopy showed that SAMP32 was associated with the inner acrosomal membrane in the principal and the equatorial segments of the sperm acrosome. By immunostaining enzyme-dissociated testicular cells, SAMP32 was localized to Golgi phase round spermatids and subsequent stages of acrosome biogenesis. Recombinant SAMP32 reacted with serum from an infertile man, suggesting that it is isoantigenic. Antibodies against recombinant SAMP32 inhibited both the binding and the fusion of human sperm to zona-free hamster eggs.

Original languageEnglish (US)
Pages (from-to)735-744
Number of pages10
JournalBiology of Reproduction
Volume66
Issue number3
DOIs
StatePublished - Jan 1 2002

Fingerprint

Spermatozoa
Testis
Membrane Proteins
Acrosome
Proteins
Immune Sera
Gels
Spermatids
Membranes
Immunoelectron Microscopy
Schizosaccharomyces
Isoelectric Point
Herpes Zoster
Cricetinae
Northern Blotting
Membrane Potentials
Serine
Eggs
Fluorescent Antibody Technique
Organism Cloning

Keywords

  • Gamete biology
  • Gametogenesis
  • Sperm
  • Spermatogenesis
  • Testis

ASJC Scopus subject areas

  • Reproductive Medicine
  • Cell Biology

Cite this

Hao, Z., Wolkowicz, M. J., Shetty, J., Klotz, K., Bolling, L., Sen, B., ... Herr, J. C. (2002). SAMP32, a testis-specific, isoantigenic sperm acrosomal membrane-associated protein. Biology of Reproduction, 66(3), 735-744. https://doi.org/10.1095/biolreprod66.3.735

SAMP32, a testis-specific, isoantigenic sperm acrosomal membrane-associated protein. / Hao, Zhonglin; Wolkowicz, Michael J.; Shetty, Jagathpala; Klotz, Kenneth; Bolling, Laura; Sen, Buer; Anne Westbrook, V.; Coonrod, Scott; Flickinger, Charles J.; Herr, John C.

In: Biology of Reproduction, Vol. 66, No. 3, 01.01.2002, p. 735-744.

Research output: Contribution to journalArticle

Hao, Z, Wolkowicz, MJ, Shetty, J, Klotz, K, Bolling, L, Sen, B, Anne Westbrook, V, Coonrod, S, Flickinger, CJ & Herr, JC 2002, 'SAMP32, a testis-specific, isoantigenic sperm acrosomal membrane-associated protein', Biology of Reproduction, vol. 66, no. 3, pp. 735-744. https://doi.org/10.1095/biolreprod66.3.735
Hao, Zhonglin ; Wolkowicz, Michael J. ; Shetty, Jagathpala ; Klotz, Kenneth ; Bolling, Laura ; Sen, Buer ; Anne Westbrook, V. ; Coonrod, Scott ; Flickinger, Charles J. ; Herr, John C. / SAMP32, a testis-specific, isoantigenic sperm acrosomal membrane-associated protein. In: Biology of Reproduction. 2002 ; Vol. 66, No. 3. pp. 735-744.
@article{4bef8bb64851491f9a3c30b376f77fcb,
title = "SAMP32, a testis-specific, isoantigenic sperm acrosomal membrane-associated protein",
abstract = "To identify novel human sperm membrane antigens, we analyzed two-dimensional gels of sperm extracts containing hydrophobic proteins that partitioned into Triton X-114. Four protein spots with isoelectric points (pls) ranging from 4.5 to 5.5 and apparent molecular weights from 32 to 34 kDa were sequenced by mass spectrometry and found to contain common peptide sequences. Cloning the corresponding cDNA revealed that these protein spots were products of a single gene (SAMP32), encoding a protein of 32 kDa with a predicted pl of 4.57. SAMP32 has a potential transmembrane domain in the carboxyl terminus and is phosphorylated in vivo on serine 256. Northern blotting of eight human tissues and RNA dot blotting of 76 human tissues showed that SAMP32 expression was testis specific. SAMP32 contained an amino terminal domain homologous to the major malarial circumsporozoite surface protein and a domain similar to that of Krp1 from Schizosaccharomyces pombe in its carboxyl terminus. The SAMP32 locus consists of seven exons on chromosome 6q15-16.2. Antiserum against recombinant SAMP32 recognized protein spots originally cored from a two-dimensional gel. This antiserum strongly stained the equatorial segment and faintly stained the acrosome cap of ejaculated human spermatozoa by immunofluorescence. Immunoelectron microscopy showed that SAMP32 was associated with the inner acrosomal membrane in the principal and the equatorial segments of the sperm acrosome. By immunostaining enzyme-dissociated testicular cells, SAMP32 was localized to Golgi phase round spermatids and subsequent stages of acrosome biogenesis. Recombinant SAMP32 reacted with serum from an infertile man, suggesting that it is isoantigenic. Antibodies against recombinant SAMP32 inhibited both the binding and the fusion of human sperm to zona-free hamster eggs.",
keywords = "Gamete biology, Gametogenesis, Sperm, Spermatogenesis, Testis",
author = "Zhonglin Hao and Wolkowicz, {Michael J.} and Jagathpala Shetty and Kenneth Klotz and Laura Bolling and Buer Sen and {Anne Westbrook}, V. and Scott Coonrod and Flickinger, {Charles J.} and Herr, {John C.}",
year = "2002",
month = "1",
day = "1",
doi = "10.1095/biolreprod66.3.735",
language = "English (US)",
volume = "66",
pages = "735--744",
journal = "Biology of Reproduction",
issn = "0006-3363",
publisher = "Society for the Study of Reproduction",
number = "3",

}

TY - JOUR

T1 - SAMP32, a testis-specific, isoantigenic sperm acrosomal membrane-associated protein

AU - Hao, Zhonglin

AU - Wolkowicz, Michael J.

AU - Shetty, Jagathpala

AU - Klotz, Kenneth

AU - Bolling, Laura

AU - Sen, Buer

AU - Anne Westbrook, V.

AU - Coonrod, Scott

AU - Flickinger, Charles J.

AU - Herr, John C.

PY - 2002/1/1

Y1 - 2002/1/1

N2 - To identify novel human sperm membrane antigens, we analyzed two-dimensional gels of sperm extracts containing hydrophobic proteins that partitioned into Triton X-114. Four protein spots with isoelectric points (pls) ranging from 4.5 to 5.5 and apparent molecular weights from 32 to 34 kDa were sequenced by mass spectrometry and found to contain common peptide sequences. Cloning the corresponding cDNA revealed that these protein spots were products of a single gene (SAMP32), encoding a protein of 32 kDa with a predicted pl of 4.57. SAMP32 has a potential transmembrane domain in the carboxyl terminus and is phosphorylated in vivo on serine 256. Northern blotting of eight human tissues and RNA dot blotting of 76 human tissues showed that SAMP32 expression was testis specific. SAMP32 contained an amino terminal domain homologous to the major malarial circumsporozoite surface protein and a domain similar to that of Krp1 from Schizosaccharomyces pombe in its carboxyl terminus. The SAMP32 locus consists of seven exons on chromosome 6q15-16.2. Antiserum against recombinant SAMP32 recognized protein spots originally cored from a two-dimensional gel. This antiserum strongly stained the equatorial segment and faintly stained the acrosome cap of ejaculated human spermatozoa by immunofluorescence. Immunoelectron microscopy showed that SAMP32 was associated with the inner acrosomal membrane in the principal and the equatorial segments of the sperm acrosome. By immunostaining enzyme-dissociated testicular cells, SAMP32 was localized to Golgi phase round spermatids and subsequent stages of acrosome biogenesis. Recombinant SAMP32 reacted with serum from an infertile man, suggesting that it is isoantigenic. Antibodies against recombinant SAMP32 inhibited both the binding and the fusion of human sperm to zona-free hamster eggs.

AB - To identify novel human sperm membrane antigens, we analyzed two-dimensional gels of sperm extracts containing hydrophobic proteins that partitioned into Triton X-114. Four protein spots with isoelectric points (pls) ranging from 4.5 to 5.5 and apparent molecular weights from 32 to 34 kDa were sequenced by mass spectrometry and found to contain common peptide sequences. Cloning the corresponding cDNA revealed that these protein spots were products of a single gene (SAMP32), encoding a protein of 32 kDa with a predicted pl of 4.57. SAMP32 has a potential transmembrane domain in the carboxyl terminus and is phosphorylated in vivo on serine 256. Northern blotting of eight human tissues and RNA dot blotting of 76 human tissues showed that SAMP32 expression was testis specific. SAMP32 contained an amino terminal domain homologous to the major malarial circumsporozoite surface protein and a domain similar to that of Krp1 from Schizosaccharomyces pombe in its carboxyl terminus. The SAMP32 locus consists of seven exons on chromosome 6q15-16.2. Antiserum against recombinant SAMP32 recognized protein spots originally cored from a two-dimensional gel. This antiserum strongly stained the equatorial segment and faintly stained the acrosome cap of ejaculated human spermatozoa by immunofluorescence. Immunoelectron microscopy showed that SAMP32 was associated with the inner acrosomal membrane in the principal and the equatorial segments of the sperm acrosome. By immunostaining enzyme-dissociated testicular cells, SAMP32 was localized to Golgi phase round spermatids and subsequent stages of acrosome biogenesis. Recombinant SAMP32 reacted with serum from an infertile man, suggesting that it is isoantigenic. Antibodies against recombinant SAMP32 inhibited both the binding and the fusion of human sperm to zona-free hamster eggs.

KW - Gamete biology

KW - Gametogenesis

KW - Sperm

KW - Spermatogenesis

KW - Testis

UR - http://www.scopus.com/inward/record.url?scp=0036187487&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036187487&partnerID=8YFLogxK

U2 - 10.1095/biolreprod66.3.735

DO - 10.1095/biolreprod66.3.735

M3 - Article

C2 - 11870081

AN - SCOPUS:0036187487

VL - 66

SP - 735

EP - 744

JO - Biology of Reproduction

JF - Biology of Reproduction

SN - 0006-3363

IS - 3

ER -