Sanguinarine induces bimodal cell death in K562 but not in high Bcl-2-expressing JM1 cells

P. Weerasinghe, S. Hallock, S. C. Tang, A. Liepins

Research output: Contribution to journalArticle

30 Scopus citations

Abstract

Our previous studies with low Bcl-2-expressing K562 cells have shown that, when treated with the putative anti-cancer drug sanguinarine, concentrations of 1.5 μg/ml induced the morphology of apoptosis or programmed cell death (PCD), while concentrations of 12.5 μg/ml induced a morphology of blister formation or blister cell death (BCD). To elucidate the possible role of Bcl-2 in this dual cell death modality induced by sanguinarine, K562 and the high Bcl-2-expressing JM1 cells were treated with sanguinarine concentrations of 1.5 μg/ml and 12.5 μg/ml respectively, and multiple parameters of their effects were studied using light and electron microscopy, terminal deoxynucleotidyl transferase (TdT) end-labeling, 51Cr release, trypan blue exclusion, propidium iodide exclusion, and annexin-V binding. In general, we found that, while K562 cells underwent PCD and BCD when treated with sanguinarine, JM1 cells failed to undergo either PCD or BCD under the same experimental conditions. Thus, the over-expression of anti-apoptotic Bcl-2 may have prevented sanguinarine from inducing PCD and BCD in JM1 cells. These results indicate that the resistance of JM1 cells to the alkaloid sanguinarine may have been due to an anti-BCD role played by Bcl-2, in addition to its widely reported anti-apoptotic role.

Original languageEnglish (US)
Pages (from-to)717-726
Number of pages10
JournalPathology Research and Practice
Volume197
Issue number11
DOIs
StatePublished - Jan 1 2001

Keywords

  • Apoptosis
  • Blebbing
  • Blister cell death
  • Chemoresistance
  • Sanguinarine

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Cell Biology

Fingerprint Dive into the research topics of 'Sanguinarine induces bimodal cell death in K562 but not in high Bcl-2-expressing JM1 cells'. Together they form a unique fingerprint.

  • Cite this