Satellite RNA-derived small interfering RNA satsiR-12 targeting the 3' untranslated region of Cucumber mosaic virus triggers viral RNAs for degradation

Hui Zhu, Cheng Guo Duan, Wei Na Hou, Quansheng Du, Dian Qiu Lv, Rong Xiang Fang, Hui Shan Guo

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

RNA silencing provides protection against RNA viruses by targeting both the helper virus and its satellite RNA (satRNA). Virus-derived small interfering RNAs (vsiRNAs) bound with Argonaute (AGO) proteins are presumed participants in the silencing process. Here, we show that a vsiRNA targeted to virus RNAs triggers the host RNA-dependent RNA polymerase 6 (RDR6)-mediated degradation of viral RNAs. We confirmed that satRNA-derived small interfering RNAs (satsiRNAs) could be associated with different AGO proteins in planta. The most frequently cloned satsiRNA, satsiR-12, was predicted to imperfectly match to Cucumber mosaic virus (CMV) RNAs in the upstream area of the 3' untranslated region (3' UTR). Moreover, an artificial satsiR-12 (asatsiR-12) mediated cleavage of a green fluorescent protein (GFP) sensor construct harboring the satsiR-12 target site. asatsiR-12 also mediated reduction of viral RNAs in 2b-deficient CMV (CMVΔ2b)-infected Nicotiana benthamiana. The reduction was not observed in CMVΔ2b-infected RDR6i plants, in which RDR6 was silenced. Following infection with 2b-containing CMV, the reduction in viral RNAs was not observed in plants of either genotype, indicating that the asatsiR-12-mediated reduction of viral RNAs in the presence of RDR6 was inhibited by the 2b protein. Our results suggest that satsiR-12 targeting the 3' UTR of CMV RNAs triggered RDR6-dependent antiviral silencing. Competition experiments with wild-type CMV RNAs and antisatsiR- 12 mutant RNA1 in the presence of 2b and satRNA demonstrate the inhibitory effect of the 2b protein on the satsiR-12-related degradation of CMV RNAs, revealing a substantial suppressor function of the 2b protein in native CMV infection. Our data provide evidence for the important biological functions of satsiRNAs in homeostatic interactions among the host, virus, and satRNA in the final outcome of viral infection.

Original languageEnglish (US)
Pages (from-to)13384-13397
Number of pages14
JournalJournal of Virology
Volume85
Issue number24
DOIs
StatePublished - Dec 1 2011

Fingerprint

Satellite RNAs
satellite RNA
Cucumovirus
Cucumber mosaic virus
RNA Stability
Viral RNA
3' Untranslated Regions
3' untranslated regions
small interfering RNA
Small Interfering RNA
RNA-directed RNA polymerase
RNA Replicase
RNA
degradation
Argonaute Proteins
RNA Viruses
Virus Diseases
proteins
viruses
Helper Viruses

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Satellite RNA-derived small interfering RNA satsiR-12 targeting the 3' untranslated region of Cucumber mosaic virus triggers viral RNAs for degradation. / Zhu, Hui; Duan, Cheng Guo; Hou, Wei Na; Du, Quansheng; Lv, Dian Qiu; Fang, Rong Xiang; Guo, Hui Shan.

In: Journal of Virology, Vol. 85, No. 24, 01.12.2011, p. 13384-13397.

Research output: Contribution to journalArticle

Zhu, Hui ; Duan, Cheng Guo ; Hou, Wei Na ; Du, Quansheng ; Lv, Dian Qiu ; Fang, Rong Xiang ; Guo, Hui Shan. / Satellite RNA-derived small interfering RNA satsiR-12 targeting the 3' untranslated region of Cucumber mosaic virus triggers viral RNAs for degradation. In: Journal of Virology. 2011 ; Vol. 85, No. 24. pp. 13384-13397.
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AU - Lv, Dian Qiu

AU - Fang, Rong Xiang

AU - Guo, Hui Shan

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