Scanning force microscopy images of cytochrome c oxidase immobilized in an electrode-supported lipid bilayer membrane

James Burgess, Vivian W. Jones, Marc D. Porter, Melissa C. Rhoten, Fred M. Hawkridge

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Scanning force microscopic images of cytochrome c oxidase immobilized within an electrode-supported lipid bilayer membrane are reported. These images represent the first direct evidence that the microscopic architecture of this important model system effectively mimics the microstructure proposed for the inner mitochondrial membrane. The images reveal (1) that the oxidase is present as monomers and small aggregates within the supported membrane and (2) that the oxidase constitutes ∼20% of the lipid bilayer membrane. The latter finding allows an estimation of the lower limit for the minimum turnover rate (∼0.5 electrons/s) required for the transition of the oxidase from its resting to pulsed kinetic state.

Original languageEnglish (US)
Pages (from-to)6628-6631
Number of pages4
JournalLangmuir
Volume14
Issue number23
StatePublished - Nov 10 1998

Fingerprint

Lipid bilayers
oxidase
cytochromes
Electron Transport Complex IV
Membrane Lipids
lipids
Atomic force microscopy
Oxidoreductases
membranes
microscopy
Membranes
Electrodes
scanning
electrodes
Electron transitions
Monomers
Scanning
Microstructure
Kinetics
Electrons

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Spectroscopy
  • Electrochemistry

Cite this

Burgess, J., Jones, V. W., Porter, M. D., Rhoten, M. C., & Hawkridge, F. M. (1998). Scanning force microscopy images of cytochrome c oxidase immobilized in an electrode-supported lipid bilayer membrane. Langmuir, 14(23), 6628-6631.

Scanning force microscopy images of cytochrome c oxidase immobilized in an electrode-supported lipid bilayer membrane. / Burgess, James; Jones, Vivian W.; Porter, Marc D.; Rhoten, Melissa C.; Hawkridge, Fred M.

In: Langmuir, Vol. 14, No. 23, 10.11.1998, p. 6628-6631.

Research output: Contribution to journalArticle

Burgess, J, Jones, VW, Porter, MD, Rhoten, MC & Hawkridge, FM 1998, 'Scanning force microscopy images of cytochrome c oxidase immobilized in an electrode-supported lipid bilayer membrane', Langmuir, vol. 14, no. 23, pp. 6628-6631.
Burgess J, Jones VW, Porter MD, Rhoten MC, Hawkridge FM. Scanning force microscopy images of cytochrome c oxidase immobilized in an electrode-supported lipid bilayer membrane. Langmuir. 1998 Nov 10;14(23):6628-6631.
Burgess, James ; Jones, Vivian W. ; Porter, Marc D. ; Rhoten, Melissa C. ; Hawkridge, Fred M. / Scanning force microscopy images of cytochrome c oxidase immobilized in an electrode-supported lipid bilayer membrane. In: Langmuir. 1998 ; Vol. 14, No. 23. pp. 6628-6631.
@article{8c0eaa9a114248e6972d7ae2522c4f6d,
title = "Scanning force microscopy images of cytochrome c oxidase immobilized in an electrode-supported lipid bilayer membrane",
abstract = "Scanning force microscopic images of cytochrome c oxidase immobilized within an electrode-supported lipid bilayer membrane are reported. These images represent the first direct evidence that the microscopic architecture of this important model system effectively mimics the microstructure proposed for the inner mitochondrial membrane. The images reveal (1) that the oxidase is present as monomers and small aggregates within the supported membrane and (2) that the oxidase constitutes ∼20{\%} of the lipid bilayer membrane. The latter finding allows an estimation of the lower limit for the minimum turnover rate (∼0.5 electrons/s) required for the transition of the oxidase from its resting to pulsed kinetic state.",
author = "James Burgess and Jones, {Vivian W.} and Porter, {Marc D.} and Rhoten, {Melissa C.} and Hawkridge, {Fred M.}",
year = "1998",
month = "11",
day = "10",
language = "English (US)",
volume = "14",
pages = "6628--6631",
journal = "Langmuir",
issn = "0743-7463",
publisher = "American Chemical Society",
number = "23",

}

TY - JOUR

T1 - Scanning force microscopy images of cytochrome c oxidase immobilized in an electrode-supported lipid bilayer membrane

AU - Burgess, James

AU - Jones, Vivian W.

AU - Porter, Marc D.

AU - Rhoten, Melissa C.

AU - Hawkridge, Fred M.

PY - 1998/11/10

Y1 - 1998/11/10

N2 - Scanning force microscopic images of cytochrome c oxidase immobilized within an electrode-supported lipid bilayer membrane are reported. These images represent the first direct evidence that the microscopic architecture of this important model system effectively mimics the microstructure proposed for the inner mitochondrial membrane. The images reveal (1) that the oxidase is present as monomers and small aggregates within the supported membrane and (2) that the oxidase constitutes ∼20% of the lipid bilayer membrane. The latter finding allows an estimation of the lower limit for the minimum turnover rate (∼0.5 electrons/s) required for the transition of the oxidase from its resting to pulsed kinetic state.

AB - Scanning force microscopic images of cytochrome c oxidase immobilized within an electrode-supported lipid bilayer membrane are reported. These images represent the first direct evidence that the microscopic architecture of this important model system effectively mimics the microstructure proposed for the inner mitochondrial membrane. The images reveal (1) that the oxidase is present as monomers and small aggregates within the supported membrane and (2) that the oxidase constitutes ∼20% of the lipid bilayer membrane. The latter finding allows an estimation of the lower limit for the minimum turnover rate (∼0.5 electrons/s) required for the transition of the oxidase from its resting to pulsed kinetic state.

UR - http://www.scopus.com/inward/record.url?scp=0032202890&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032202890&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:0032202890

VL - 14

SP - 6628

EP - 6631

JO - Langmuir

JF - Langmuir

SN - 0743-7463

IS - 23

ER -