TY - JOUR
T1 - Screening of candidate biomaterials for alveolar augmentation using a critical-size rat calvaria defect model
AU - Susin, Cristiano
AU - Lee, Jaebum
AU - Fiorini, Tiago
AU - Koo, Ki Tae
AU - Schüpbach, Peter
AU - Angst, Patricia D.M.
AU - Finger Stadler, Amanda
AU - Wikesjö, Ulf M.E.
N1 - Funding Information:
This study was supported by a grant from Nobel Biocare, Zurich, Switzerland to the Laboratory for Applied Periodontal & Craniofacial Research (LAPCR), Augusta University, Augusta, GA, USA. Drs. Cristiano Susin, Ulf ME Wikesjö, Jaebum Lee and Peter Schüpbach from time to time serve as scientific advisors and lecturers for Nobel Biocare.
Publisher Copyright:
© 2018 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd
PY - 2018/7
Y1 - 2018/7
N2 - Objective: To screen candidate biomaterials intended for alveolar augmentation relative to their potential to enhance local bone formation using a routine critical-size (ø8-mm) rat calvaria defect model. Methods: One hundred and forty male Sprague Dawley outbred rats, age 11–12 weeks, weight 325–375 g, obtained from USDA approved breeder, randomised into 14 groups of 10 animals, each received one of the following treatments: sham-surgery (empty control), Bio-Oss (bovine HA/reference control), or candidate biomaterials including bovine HA, synthetic HA/ß-TCP and calcium phosphate constructs, mineralised/demineralised human bone preparations, a ß-TCP/calcium sulphate and an HA/calcium sulphate putty. A 4-week healing interval was chosen to discern local bone formation using incandescent and polarised light microscopy. Statistical analysis used one-way ANOVA followed by Bonferroni for pairwise comparisons. Results: Candidate biomaterials all displayed biocompatibility. They exhibited limited, if any, appreciable bioerosion or biodegradation. No statistically significant differences in mean linear defect closure were observed among experimental groups, sham-surgery displaying the highest score (48.1 ± 24.3%). Sham-surgery also showed a significantly greater bone area fraction than all other groups (19.8 ± 13.9%, p <.001). The HA/calcium sulphate putty showed a significantly greater residual biomaterial area fraction than all other groups (61.1 ± 8.5%, p <.01). Conclusion: Within the limitations of this animal model, although biocompatible, none of the tested biomaterials enhanced local bone formation beyond the innate regenerative potential of this craniotomy defect.
AB - Objective: To screen candidate biomaterials intended for alveolar augmentation relative to their potential to enhance local bone formation using a routine critical-size (ø8-mm) rat calvaria defect model. Methods: One hundred and forty male Sprague Dawley outbred rats, age 11–12 weeks, weight 325–375 g, obtained from USDA approved breeder, randomised into 14 groups of 10 animals, each received one of the following treatments: sham-surgery (empty control), Bio-Oss (bovine HA/reference control), or candidate biomaterials including bovine HA, synthetic HA/ß-TCP and calcium phosphate constructs, mineralised/demineralised human bone preparations, a ß-TCP/calcium sulphate and an HA/calcium sulphate putty. A 4-week healing interval was chosen to discern local bone formation using incandescent and polarised light microscopy. Statistical analysis used one-way ANOVA followed by Bonferroni for pairwise comparisons. Results: Candidate biomaterials all displayed biocompatibility. They exhibited limited, if any, appreciable bioerosion or biodegradation. No statistically significant differences in mean linear defect closure were observed among experimental groups, sham-surgery displaying the highest score (48.1 ± 24.3%). Sham-surgery also showed a significantly greater bone area fraction than all other groups (19.8 ± 13.9%, p <.001). The HA/calcium sulphate putty showed a significantly greater residual biomaterial area fraction than all other groups (61.1 ± 8.5%, p <.01). Conclusion: Within the limitations of this animal model, although biocompatible, none of the tested biomaterials enhanced local bone formation beyond the innate regenerative potential of this craniotomy defect.
KW - bone biomaterials
KW - critical-size-defect
KW - rat calvaria
KW - tissue engineering
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U2 - 10.1111/jcpe.12904
DO - 10.1111/jcpe.12904
M3 - Article
AN - SCOPUS:85047632295
SN - 0303-6979
VL - 45
SP - 884
EP - 893
JO - Journal of Clinical Periodontology
JF - Journal of Clinical Periodontology
IS - 7
ER -