Screening of the seed region of MIR184 in Keratoconus patients from Saudi Arabia

Khaled K. Abu-Amero, Inas Helwa, Abdulrahman Al-Muammar, Shelby Strickland, Michael A. Hauser, R. Rand Allingham, Yutao Liu

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Micro-RNAs (miRNAs) are regulators of gene expression that control various biological processes. The role of many identified miRNAs is not yet resolved. Recent evidence suggests that miRNA mutations and/or misexpression may contribute to genetic disorders. Point mutations in the seed region of MIR184 have been recently identified in Keratoconus (KC) patients with or without other corneal and lens abnormalities. We investigated mutations within MIR184 in KC patients from Saudi Arabia and examined the relative expression of miR-184 and miR-205 in human cornea. Ethnically matched KC cases (n=134) were recruited and sequencing was performed using PCR-based Sanger sequencing and analyzed using the Sequencher 5.2 software. Expression of miR-184 and miR-205 was profiled in postmortem unaffected ocular tissues obtained from donors with no history of ocular diseases. miR-184 expression was 15-fold higher than that of miR-205 in cornea samples. No mutation(s) within the screened genomic region of MIR184 in KC cases was detected. This suggests that mutation in MIR184 is a rare cause of KC alone and may be more relevant to cases of KC associated with other ocular abnormalities. The increased expression of miR-184 versus miR-205 in normal cornea samples implies a possible role of miR184 in cornea development and/or corneal diseases.

Original languageEnglish (US)
Article number604508
JournalBioMed Research International
Volume2015
DOIs
StatePublished - Jan 1 2015

Fingerprint

Keratoconus
Saudi Arabia
MicroRNAs
Seed
Seeds
Screening
Cornea
Mutation
Gene expression
Lenses
Eye Abnormalities
Corneal Diseases
Tissue
Biological Phenomena
Inborn Genetic Diseases
Eye Diseases
Regulator Genes
Point Mutation
Software
Tissue Donors

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

Abu-Amero, K. K., Helwa, I., Al-Muammar, A., Strickland, S., Hauser, M. A., Allingham, R. R., & Liu, Y. (2015). Screening of the seed region of MIR184 in Keratoconus patients from Saudi Arabia. BioMed Research International, 2015, [604508]. https://doi.org/10.1155/2015/604508

Screening of the seed region of MIR184 in Keratoconus patients from Saudi Arabia. / Abu-Amero, Khaled K.; Helwa, Inas; Al-Muammar, Abdulrahman; Strickland, Shelby; Hauser, Michael A.; Allingham, R. Rand; Liu, Yutao.

In: BioMed Research International, Vol. 2015, 604508, 01.01.2015.

Research output: Contribution to journalArticle

Abu-Amero, KK, Helwa, I, Al-Muammar, A, Strickland, S, Hauser, MA, Allingham, RR & Liu, Y 2015, 'Screening of the seed region of MIR184 in Keratoconus patients from Saudi Arabia', BioMed Research International, vol. 2015, 604508. https://doi.org/10.1155/2015/604508
Abu-Amero KK, Helwa I, Al-Muammar A, Strickland S, Hauser MA, Allingham RR et al. Screening of the seed region of MIR184 in Keratoconus patients from Saudi Arabia. BioMed Research International. 2015 Jan 1;2015. 604508. https://doi.org/10.1155/2015/604508
Abu-Amero, Khaled K. ; Helwa, Inas ; Al-Muammar, Abdulrahman ; Strickland, Shelby ; Hauser, Michael A. ; Allingham, R. Rand ; Liu, Yutao. / Screening of the seed region of MIR184 in Keratoconus patients from Saudi Arabia. In: BioMed Research International. 2015 ; Vol. 2015.
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