Sea urchin USF: A helix-loop-helix protein active in embryonic ectoderm cells

Mark T. Kozlowski; Lin Gan; Judith M. Venuti; Michèle Sawadogo; William H. Klein

doi:10.1016/0012-1606(91)90280-G

Sea urchin USF: A helix-loop-helix protein active in embryonic ectoderm cells

Mark T. Kozlowski, Lin Gan, Judith M. Venuti, Michèle Sawadogo, William H. Klein

Research output: Contribution to journal › Article › peer-review

32 Scopus citations

Abstract

We previously characterized a DNA-binding factor in nuclear extracts of Strongylocentrotus purpuratus embryos that bound Spec gene promoters, was ectoderm specific, and had properties similar to the vertebrate transcription factor USF. Here we describe a cDNA clone, suUSF, isolated from an S. purpuratus cDNA library, with sequence homology to human USF. Spec gene promoter fragments formed sequence-specific complexes with suUSF, and antibodies against suUSF inhibited binding activity in nuclear extracts. Reaction of USF-site containing probes with filter-bound nuclear proteins demonstrated that suUSF binding activity was enriched in ectoderm cells, and immunoblotting showed a similar ectoderm enrichment. These data demonstrated that suUSF was responsible for the ectoderm-specific activity observed in sea urchin extracts.

Original language	English (US)
Pages (from-to)	625-630
Number of pages	6
Journal	Developmental Biology
Volume	148
Issue number	2
DOIs	https://doi.org/10.1016/0012-1606(91)90280-G
State	Published - Dec 1991
Externally published	Yes

ASJC Scopus subject areas

Molecular Biology
Developmental Biology
Cell Biology

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10.1016/0012-1606(91)90280-G

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@article{c4097895a9e34dafa4a6218a514cfbed,

title = "Sea urchin USF: A helix-loop-helix protein active in embryonic ectoderm cells",

abstract = "We previously characterized a DNA-binding factor in nuclear extracts of Strongylocentrotus purpuratus embryos that bound Spec gene promoters, was ectoderm specific, and had properties similar to the vertebrate transcription factor USF. Here we describe a cDNA clone, suUSF, isolated from an S. purpuratus cDNA library, with sequence homology to human USF. Spec gene promoter fragments formed sequence-specific complexes with suUSF, and antibodies against suUSF inhibited binding activity in nuclear extracts. Reaction of USF-site containing probes with filter-bound nuclear proteins demonstrated that suUSF binding activity was enriched in ectoderm cells, and immunoblotting showed a similar ectoderm enrichment. These data demonstrated that suUSF was responsible for the ectoderm-specific activity observed in sea urchin extracts.",

author = "Kozlowski, {Mark T.} and Lin Gan and Venuti, {Judith M.} and Mich{\`e}le Sawadogo and Klein, {William H.}",

note = "Funding Information: We thank Drs. Craig Tomlinson, Gary Wessel, and Michael Perry for advice and encouragement and Robin Barrett for technical assistance. This work was supported by NIH grants to W.H.K. (HD22619) and M.S. (GM38212).",

year = "1991",

month = dec,

doi = "10.1016/0012-1606(91)90280-G",

language = "English (US)",

volume = "148",

pages = "625--630",

journal = "Developmental Biology",

issn = "0012-1606",

publisher = "Academic Press Inc.",

number = "2",

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TY - JOUR

T1 - Sea urchin USF

T2 - A helix-loop-helix protein active in embryonic ectoderm cells

AU - Kozlowski, Mark T.

AU - Gan, Lin

AU - Venuti, Judith M.

AU - Sawadogo, Michèle

AU - Klein, William H.

N1 - Funding Information: We thank Drs. Craig Tomlinson, Gary Wessel, and Michael Perry for advice and encouragement and Robin Barrett for technical assistance. This work was supported by NIH grants to W.H.K. (HD22619) and M.S. (GM38212).

PY - 1991/12

Y1 - 1991/12

N2 - We previously characterized a DNA-binding factor in nuclear extracts of Strongylocentrotus purpuratus embryos that bound Spec gene promoters, was ectoderm specific, and had properties similar to the vertebrate transcription factor USF. Here we describe a cDNA clone, suUSF, isolated from an S. purpuratus cDNA library, with sequence homology to human USF. Spec gene promoter fragments formed sequence-specific complexes with suUSF, and antibodies against suUSF inhibited binding activity in nuclear extracts. Reaction of USF-site containing probes with filter-bound nuclear proteins demonstrated that suUSF binding activity was enriched in ectoderm cells, and immunoblotting showed a similar ectoderm enrichment. These data demonstrated that suUSF was responsible for the ectoderm-specific activity observed in sea urchin extracts.

AB - We previously characterized a DNA-binding factor in nuclear extracts of Strongylocentrotus purpuratus embryos that bound Spec gene promoters, was ectoderm specific, and had properties similar to the vertebrate transcription factor USF. Here we describe a cDNA clone, suUSF, isolated from an S. purpuratus cDNA library, with sequence homology to human USF. Spec gene promoter fragments formed sequence-specific complexes with suUSF, and antibodies against suUSF inhibited binding activity in nuclear extracts. Reaction of USF-site containing probes with filter-bound nuclear proteins demonstrated that suUSF binding activity was enriched in ectoderm cells, and immunoblotting showed a similar ectoderm enrichment. These data demonstrated that suUSF was responsible for the ectoderm-specific activity observed in sea urchin extracts.

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Sea urchin USF: A helix-loop-helix protein active in embryonic ectoderm cells

Abstract

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