Sealing properties of a self-etching primer system to normal caries-affected and caries-infected dentin.

Kwang Won Lee, H. H. Son, Masatoshi Yoshiyama, Franklin Chi Meng Tay, Ricardo M. Carvalho, David H. Pashley

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10 Scopus citations

Abstract

PURPOSE: To compare the ability of an experimental antibacterial self-etching primer adhesive system to seal exposure sites in normal, caries-affected and caries-infected human dentin. METHODS: 30 extracted human third molars were used within 1 month of extraction. 10 intact normal teeth comprised the normal group. 20 teeth with occlusal caries that radiographically extended halfway to the pulp were excavated using caries-detector solution (CDS) and a #4 round carbide bur in a slowspeed handpiece. Half of those teeth were fully excavated free of CDS-stained material without exposing the pulp, and were designated as the caries-affected dentin group. The remaining 10 teeth were excavated as close to the pulp as possible without obtaining an exposure, but whose dentin continued to stain red with CDS; this group was designated as the caries-infected dentin group. The remaining dentin thickness in all of the specimens in the other two groups was then reduced to the same extent as the caries-infected group. Direct exposures of the pulp chamber were made with a 1/4 round bur in the normal dentin or a 25 gauge needle in the other two groups. After measuring the fluid flow through the exposure, the sites were then sealed with an experimental antibacterial fluoride-containing self-etching primer adhesive systems (ABF). Fluid conductance was remeasured every week for 16 weeks. RESULTS: The fluid conductance through the exposure fell 99% in all groups following resin sealing. The seals of normal and caries-affected dentin remained relatively stable over the 16 weeks, while the seals of caries-infected dentin gradually deteriorated, reaching significance at 8 weeks. TEM examination revealed very thin (ca. 0.5 mm) hybrid layers in normal dentin, 3-4 microm thick hybrid layers in caries-affected dentin and 40 microm thick hybrid layers in caries-infected dentin. The tubules of caries-infected dentin were enlarged and filled with bacteria. Resin tags passed around these bacteria in the top 20-40 microm thereby encapsulating them in resin.

Original languageEnglish (US)
Pages (from-to)68A-72A
JournalAmerican journal of dentistry
Volume16 Spec No
StatePublished - Sep 2003

ASJC Scopus subject areas

  • Dentistry(all)

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    Lee, K. W., Son, H. H., Yoshiyama, M., Tay, F. C. M., Carvalho, R. M., & Pashley, D. H. (2003). Sealing properties of a self-etching primer system to normal caries-affected and caries-infected dentin. American journal of dentistry, 16 Spec No, 68A-72A.