Secretin is not necessary for exocrine pancreatic development and growth in mice

Maria Dolors Sans, Maria Eugenia Sabbatini, Stephen A. Ernst, Louis G. D'Alecy, Ichiko Nishijima, John A. Williams

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Adaptive exocrine pancreatic growth is mediated primarily by dietary protein and the gastrointestinal hormone cholecystokinin (CCK). Feeding trypsin inhibitors such as camostat (FOY-305) is known to induce CCK release and stimulate pancreatic growth. However, camostat has also been reported to stimulate secretin release and, because secretin often potentiates the action of CCK, it could participate in the growth response. Our aim was to test the role of secretin in pancreatic development and adaptive growth through the use of C57BL/6 mice with genetic deletion of secretin or secretin receptor. The lack of secretin in the intestine or the secretin receptor in the pancreas was confirmed by RT-PCR. Other related components, such as vasoactive intestinal polypeptide (VIP) receptors (VPAC 1 and VPAC 2), were not affected. Secretin increased cAMP levels in acini from wild-type (WT) mice but had no effect on acini from secretin receptor-deleted mice, whereas VIP and forskolin still induced a normal response. Secretin in vivo failed to induce fluid secretion in receptor-deficient mice. The pancreas of secretin or secretin receptor-deficient mice was of normal size and histology, indicating that secretin is not necessary for normal pancreatic differentiation or maintenance. When WT mice were fed 0.1% camostat in powdered chow, the pancreas doubled in size in 1 wk, accompanied by parallel increases in protein and DNA. Camostat-fed littermate secretin and secretin receptor-deficient mice had similar pancreatic mass to WT mice. These results indicate that secretin is not required for normal pancreatic development or adaptive growth mediated by CCK.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Gastrointestinal and Liver Physiology
Volume301
Issue number5
DOIs
StatePublished - Nov 1 2011
Externally publishedYes

Fingerprint

Secretin
Growth and Development
Cholecystokinin
Pancreas
Vasoactive Intestinal Peptide
Growth
Gastrointestinal Hormones
Fluids and Secretions
Trypsin Inhibitors
Dietary Proteins
Colforsin
Inbred C57BL Mouse
Intestines
Histology
Maintenance
secretin receptor
camostat

Keywords

  • Cholecystokinin
  • Pancreatic growth
  • Secretin
  • Trypsin inhibitor

ASJC Scopus subject areas

  • Physiology
  • Hepatology
  • Gastroenterology
  • Physiology (medical)

Cite this

Secretin is not necessary for exocrine pancreatic development and growth in mice. / Sans, Maria Dolors; Sabbatini, Maria Eugenia; Ernst, Stephen A.; D'Alecy, Louis G.; Nishijima, Ichiko; Williams, John A.

In: American Journal of Physiology - Gastrointestinal and Liver Physiology, Vol. 301, No. 5, 01.11.2011.

Research output: Contribution to journalArticle

Sans, Maria Dolors ; Sabbatini, Maria Eugenia ; Ernst, Stephen A. ; D'Alecy, Louis G. ; Nishijima, Ichiko ; Williams, John A. / Secretin is not necessary for exocrine pancreatic development and growth in mice. In: American Journal of Physiology - Gastrointestinal and Liver Physiology. 2011 ; Vol. 301, No. 5.
@article{73eba505f0074686962d3f307f9171e8,
title = "Secretin is not necessary for exocrine pancreatic development and growth in mice",
abstract = "Adaptive exocrine pancreatic growth is mediated primarily by dietary protein and the gastrointestinal hormone cholecystokinin (CCK). Feeding trypsin inhibitors such as camostat (FOY-305) is known to induce CCK release and stimulate pancreatic growth. However, camostat has also been reported to stimulate secretin release and, because secretin often potentiates the action of CCK, it could participate in the growth response. Our aim was to test the role of secretin in pancreatic development and adaptive growth through the use of C57BL/6 mice with genetic deletion of secretin or secretin receptor. The lack of secretin in the intestine or the secretin receptor in the pancreas was confirmed by RT-PCR. Other related components, such as vasoactive intestinal polypeptide (VIP) receptors (VPAC 1 and VPAC 2), were not affected. Secretin increased cAMP levels in acini from wild-type (WT) mice but had no effect on acini from secretin receptor-deleted mice, whereas VIP and forskolin still induced a normal response. Secretin in vivo failed to induce fluid secretion in receptor-deficient mice. The pancreas of secretin or secretin receptor-deficient mice was of normal size and histology, indicating that secretin is not necessary for normal pancreatic differentiation or maintenance. When WT mice were fed 0.1{\%} camostat in powdered chow, the pancreas doubled in size in 1 wk, accompanied by parallel increases in protein and DNA. Camostat-fed littermate secretin and secretin receptor-deficient mice had similar pancreatic mass to WT mice. These results indicate that secretin is not required for normal pancreatic development or adaptive growth mediated by CCK.",
keywords = "Cholecystokinin, Pancreatic growth, Secretin, Trypsin inhibitor",
author = "Sans, {Maria Dolors} and Sabbatini, {Maria Eugenia} and Ernst, {Stephen A.} and D'Alecy, {Louis G.} and Ichiko Nishijima and Williams, {John A.}",
year = "2011",
month = "11",
day = "1",
doi = "10.1152/ajpgi.00245.2011",
language = "English (US)",
volume = "301",
journal = "American journal of physiology. Gastrointestinal and liver physiology",
issn = "0193-1857",
publisher = "American Physiological Society",
number = "5",

}

TY - JOUR

T1 - Secretin is not necessary for exocrine pancreatic development and growth in mice

AU - Sans, Maria Dolors

AU - Sabbatini, Maria Eugenia

AU - Ernst, Stephen A.

AU - D'Alecy, Louis G.

AU - Nishijima, Ichiko

AU - Williams, John A.

PY - 2011/11/1

Y1 - 2011/11/1

N2 - Adaptive exocrine pancreatic growth is mediated primarily by dietary protein and the gastrointestinal hormone cholecystokinin (CCK). Feeding trypsin inhibitors such as camostat (FOY-305) is known to induce CCK release and stimulate pancreatic growth. However, camostat has also been reported to stimulate secretin release and, because secretin often potentiates the action of CCK, it could participate in the growth response. Our aim was to test the role of secretin in pancreatic development and adaptive growth through the use of C57BL/6 mice with genetic deletion of secretin or secretin receptor. The lack of secretin in the intestine or the secretin receptor in the pancreas was confirmed by RT-PCR. Other related components, such as vasoactive intestinal polypeptide (VIP) receptors (VPAC 1 and VPAC 2), were not affected. Secretin increased cAMP levels in acini from wild-type (WT) mice but had no effect on acini from secretin receptor-deleted mice, whereas VIP and forskolin still induced a normal response. Secretin in vivo failed to induce fluid secretion in receptor-deficient mice. The pancreas of secretin or secretin receptor-deficient mice was of normal size and histology, indicating that secretin is not necessary for normal pancreatic differentiation or maintenance. When WT mice were fed 0.1% camostat in powdered chow, the pancreas doubled in size in 1 wk, accompanied by parallel increases in protein and DNA. Camostat-fed littermate secretin and secretin receptor-deficient mice had similar pancreatic mass to WT mice. These results indicate that secretin is not required for normal pancreatic development or adaptive growth mediated by CCK.

AB - Adaptive exocrine pancreatic growth is mediated primarily by dietary protein and the gastrointestinal hormone cholecystokinin (CCK). Feeding trypsin inhibitors such as camostat (FOY-305) is known to induce CCK release and stimulate pancreatic growth. However, camostat has also been reported to stimulate secretin release and, because secretin often potentiates the action of CCK, it could participate in the growth response. Our aim was to test the role of secretin in pancreatic development and adaptive growth through the use of C57BL/6 mice with genetic deletion of secretin or secretin receptor. The lack of secretin in the intestine or the secretin receptor in the pancreas was confirmed by RT-PCR. Other related components, such as vasoactive intestinal polypeptide (VIP) receptors (VPAC 1 and VPAC 2), were not affected. Secretin increased cAMP levels in acini from wild-type (WT) mice but had no effect on acini from secretin receptor-deleted mice, whereas VIP and forskolin still induced a normal response. Secretin in vivo failed to induce fluid secretion in receptor-deficient mice. The pancreas of secretin or secretin receptor-deficient mice was of normal size and histology, indicating that secretin is not necessary for normal pancreatic differentiation or maintenance. When WT mice were fed 0.1% camostat in powdered chow, the pancreas doubled in size in 1 wk, accompanied by parallel increases in protein and DNA. Camostat-fed littermate secretin and secretin receptor-deficient mice had similar pancreatic mass to WT mice. These results indicate that secretin is not required for normal pancreatic development or adaptive growth mediated by CCK.

KW - Cholecystokinin

KW - Pancreatic growth

KW - Secretin

KW - Trypsin inhibitor

UR - http://www.scopus.com/inward/record.url?scp=80055048597&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=80055048597&partnerID=8YFLogxK

U2 - 10.1152/ajpgi.00245.2011

DO - 10.1152/ajpgi.00245.2011

M3 - Article

VL - 301

JO - American journal of physiology. Gastrointestinal and liver physiology

JF - American journal of physiology. Gastrointestinal and liver physiology

SN - 0193-1857

IS - 5

ER -