Secretory product expression during Clara cell differentiation in the rabbit and rat

W. V. Cardoso, L. G. Stewart, K. E. Pinkerton, C. Ji, G. E.R. Hook, G. Singh, S. L. Katyal, W. M. Thurlbeck, C. G. Plopper

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

One function of the nonciliated (Clara) cells of bronchiolar epithelium is to synthesize, store, and release small-molecular-mass (6-12 kDa) secretory proteins or Clara cell secretory protein (CCSP). This study compares the emergence of this secretory function during Clara cell differentiation in rabbits and rats. Lungs of fetal and postnatal animals were evaluated by ultrastructural morphometry and immunohistochemistry. Secretory granules were rarely seen in perinatal animals and increased to adult levels of abundance earlier in rats (1 wk postnatal) than in rabbits (3-4 wk). In contrast, rough endoplasmic reticulum was abundant in perinatal animals and decreased with age. Antibodies raised against CCSP revealed little CCSP in fetal animals; however, after birth CCSP increased to adult levels earlier in rats (1 wk postnatal) than in rabbits (3 wk). We conclude that the maturation of Clara cell secretory function 1) occurs postnatally, 2) involves a decrease in biosynthetic organelles, 3) shows close association between CCSP expression and secretory granule abundance, and 4) varies by species in timing and cellular abundance of biosynthetic machinery.

Original languageEnglish (US)
Pages (from-to)L543-L552
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Volume264
Issue number6 8-6
StatePublished - Jan 1 1993
Externally publishedYes

Fingerprint

Uteroglobin
Cell Differentiation
Rabbits
Secretory Vesicles
Rough Endoplasmic Reticulum
Organelles
Epithelium
Immunohistochemistry
Parturition
Lung
Antibodies
Proteins

Keywords

  • Clara cell secretory protein
  • bronchiolar differentiation
  • lung development

ASJC Scopus subject areas

  • Physiology
  • Pulmonary and Respiratory Medicine
  • Physiology (medical)
  • Cell Biology

Cite this

Cardoso, W. V., Stewart, L. G., Pinkerton, K. E., Ji, C., Hook, G. E. R., Singh, G., ... Plopper, C. G. (1993). Secretory product expression during Clara cell differentiation in the rabbit and rat. American Journal of Physiology - Lung Cellular and Molecular Physiology, 264(6 8-6), L543-L552.

Secretory product expression during Clara cell differentiation in the rabbit and rat. / Cardoso, W. V.; Stewart, L. G.; Pinkerton, K. E.; Ji, C.; Hook, G. E.R.; Singh, G.; Katyal, S. L.; Thurlbeck, W. M.; Plopper, C. G.

In: American Journal of Physiology - Lung Cellular and Molecular Physiology, Vol. 264, No. 6 8-6, 01.01.1993, p. L543-L552.

Research output: Contribution to journalArticle

Cardoso, WV, Stewart, LG, Pinkerton, KE, Ji, C, Hook, GER, Singh, G, Katyal, SL, Thurlbeck, WM & Plopper, CG 1993, 'Secretory product expression during Clara cell differentiation in the rabbit and rat', American Journal of Physiology - Lung Cellular and Molecular Physiology, vol. 264, no. 6 8-6, pp. L543-L552.
Cardoso, W. V. ; Stewart, L. G. ; Pinkerton, K. E. ; Ji, C. ; Hook, G. E.R. ; Singh, G. ; Katyal, S. L. ; Thurlbeck, W. M. ; Plopper, C. G. / Secretory product expression during Clara cell differentiation in the rabbit and rat. In: American Journal of Physiology - Lung Cellular and Molecular Physiology. 1993 ; Vol. 264, No. 6 8-6. pp. L543-L552.
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