Secretory product expression during Clara cell differentiation in the rabbit and rat

W. V. Cardoso; L. G. Stewart; K. E. Pinkerton; C. Ji; G. E.R. Hook; G. Singh; S. L. Katyal; W. M. Thurlbeck; C. G. Plopper

doi:10.1152/ajplung.1993.264.6.l543

Secretory product expression during Clara cell differentiation in the rabbit and rat

W. V. Cardoso, L. G. Stewart, K. E. Pinkerton, C. Ji, G. E.R. Hook, G. Singh, S. L. Katyal, W. M. Thurlbeck, C. G. Plopper

Research output: Contribution to journal › Article › peer-review

30 Scopus citations

Abstract

One function of the nonciliated (Clara) cells of bronchiolar epithelium is to synthesize, store, and release small-molecular-mass (6-12 kDa) secretory proteins or Clara cell secretory protein (CCSP). This study compares the emergence of this secretory function during Clara cell differentiation in rabbits and rats. Lungs of fetal and postnatal animals were evaluated by ultrastructural morphometry and immunohistochemistry. Secretory granules were rarely seen in perinatal animals and increased to adult levels of abundance earlier in rats (1 wk postnatal) than in rabbits (3-4 wk). In contrast, rough endoplasmic reticulum was abundant in perinatal animals and decreased with age. Antibodies raised against CCSP revealed little CCSP in fetal animals; however, after birth CCSP increased to adult levels earlier in rats (1 wk postnatal) than in rabbits (3 wk). We conclude that the maturation of Clara cell secretory function 1) occurs postnatally, 2) involves a decrease in biosynthetic organelles, 3) shows close association between CCSP expression and secretory granule abundance, and 4) varies by species in timing and cellular abundance of biosynthetic machinery.

Original language	English (US)
Pages (from-to)	L543-L552
Journal	American Journal of Physiology - Lung Cellular and Molecular Physiology
Volume	264
Issue number	6 8-6
DOIs	https://doi.org/10.1152/ajplung.1993.264.6.l543
State	Published - 1993
Externally published	Yes

Keywords

Clara cell secretory protein
bronchiolar differentiation
lung development

ASJC Scopus subject areas

Physiology
Pulmonary and Respiratory Medicine
Physiology (medical)
Cell Biology

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10.1152/ajplung.1993.264.6.l543

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Cardoso, W. V., Stewart, L. G., Pinkerton, K. E., Ji, C., Hook, G. E. R., Singh, G., Katyal, S. L., Thurlbeck, W. M., & Plopper, C. G. (1993). Secretory product expression during Clara cell differentiation in the rabbit and rat. American Journal of Physiology - Lung Cellular and Molecular Physiology, 264(6 8-6), L543-L552. https://doi.org/10.1152/ajplung.1993.264.6.l543

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abstract = "One function of the nonciliated (Clara) cells of bronchiolar epithelium is to synthesize, store, and release small-molecular-mass (6-12 kDa) secretory proteins or Clara cell secretory protein (CCSP). This study compares the emergence of this secretory function during Clara cell differentiation in rabbits and rats. Lungs of fetal and postnatal animals were evaluated by ultrastructural morphometry and immunohistochemistry. Secretory granules were rarely seen in perinatal animals and increased to adult levels of abundance earlier in rats (1 wk postnatal) than in rabbits (3-4 wk). In contrast, rough endoplasmic reticulum was abundant in perinatal animals and decreased with age. Antibodies raised against CCSP revealed little CCSP in fetal animals; however, after birth CCSP increased to adult levels earlier in rats (1 wk postnatal) than in rabbits (3 wk). We conclude that the maturation of Clara cell secretory function 1) occurs postnatally, 2) involves a decrease in biosynthetic organelles, 3) shows close association between CCSP expression and secretory granule abundance, and 4) varies by species in timing and cellular abundance of biosynthetic machinery.",

keywords = "Clara cell secretory protein, bronchiolar differentiation, lung development",

author = "Cardoso, {W. V.} and Stewart, {L. G.} and Pinkerton, {K. E.} and C. Ji and Hook, {G. E.R.} and G. Singh and Katyal, {S. L.} and Thurlbeck, {W. M.} and Plopper, {C. G.}",

year = "1993",

doi = "10.1152/ajplung.1993.264.6.l543",

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AU - Stewart, L. G.

AU - Pinkerton, K. E.

AU - Ji, C.

AU - Hook, G. E.R.

AU - Singh, G.

AU - Katyal, S. L.

AU - Thurlbeck, W. M.

AU - Plopper, C. G.

PY - 1993

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AB - One function of the nonciliated (Clara) cells of bronchiolar epithelium is to synthesize, store, and release small-molecular-mass (6-12 kDa) secretory proteins or Clara cell secretory protein (CCSP). This study compares the emergence of this secretory function during Clara cell differentiation in rabbits and rats. Lungs of fetal and postnatal animals were evaluated by ultrastructural morphometry and immunohistochemistry. Secretory granules were rarely seen in perinatal animals and increased to adult levels of abundance earlier in rats (1 wk postnatal) than in rabbits (3-4 wk). In contrast, rough endoplasmic reticulum was abundant in perinatal animals and decreased with age. Antibodies raised against CCSP revealed little CCSP in fetal animals; however, after birth CCSP increased to adult levels earlier in rats (1 wk postnatal) than in rabbits (3 wk). We conclude that the maturation of Clara cell secretory function 1) occurs postnatally, 2) involves a decrease in biosynthetic organelles, 3) shows close association between CCSP expression and secretory granule abundance, and 4) varies by species in timing and cellular abundance of biosynthetic machinery.

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Secretory product expression during Clara cell differentiation in the rabbit and rat

Abstract

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ASJC Scopus subject areas

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