Secretory proteins of the lung in rodents: Immunocytochemistry

G. Singh, S. L. Katyal, J. M. Ward, S. A. Gottron, M. L. Wong-Chong, E. J. Riley

Research output: Contribution to journalArticle

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Abstract

The reactivity of rabbit antisera to rat lung secretory proteins with other rodent species was evaluated by immunocytochemistry. Rabbit anti-rat surfactant apoprotein antiserum reacts with the cytoplasm of rat, mouse, and hamster type II pneumocytes and is specific for these cells. Rabbit antiserum to rat Clara cell secretory proteins stains rat, mouse, and hamster Clara cells. Rabbit antisera specific to the two antigenic types of rat Clara cell antigens were also both reactive with rat, mouse, and hamster Clara cells. An antiserum to the non-serum protein of hamster lung lavage was also prepared and showed to be specifically reactive with hamster Clara cells. The availability of specific reagents for secretory proteins of rodent lungs is expected to facilitate studies of the respective cell types in various pathologic states.

Original languageEnglish (US)
Pages (from-to)564-658
Number of pages95
JournalJournal of Histochemistry and Cytochemistry
Volume33
Issue number6
StatePublished - Sep 25 1985

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Rodentia
Immunohistochemistry
Cricetinae
Lung
Immune Sera
Proteins
Rabbits
Uteroglobin
Alveolar Epithelial Cells
Apoproteins
Bronchoalveolar Lavage
Surface-Active Agents
Cytoplasm
Coloring Agents

ASJC Scopus subject areas

  • Anatomy
  • Histology

Cite this

Singh, G., Katyal, S. L., Ward, J. M., Gottron, S. A., Wong-Chong, M. L., & Riley, E. J. (1985). Secretory proteins of the lung in rodents: Immunocytochemistry. Journal of Histochemistry and Cytochemistry, 33(6), 564-658.

Secretory proteins of the lung in rodents : Immunocytochemistry. / Singh, G.; Katyal, S. L.; Ward, J. M.; Gottron, S. A.; Wong-Chong, M. L.; Riley, E. J.

In: Journal of Histochemistry and Cytochemistry, Vol. 33, No. 6, 25.09.1985, p. 564-658.

Research output: Contribution to journalArticle

Singh, G, Katyal, SL, Ward, JM, Gottron, SA, Wong-Chong, ML & Riley, EJ 1985, 'Secretory proteins of the lung in rodents: Immunocytochemistry', Journal of Histochemistry and Cytochemistry, vol. 33, no. 6, pp. 564-658.
Singh G, Katyal SL, Ward JM, Gottron SA, Wong-Chong ML, Riley EJ. Secretory proteins of the lung in rodents: Immunocytochemistry. Journal of Histochemistry and Cytochemistry. 1985 Sep 25;33(6):564-658.
Singh, G. ; Katyal, S. L. ; Ward, J. M. ; Gottron, S. A. ; Wong-Chong, M. L. ; Riley, E. J. / Secretory proteins of the lung in rodents : Immunocytochemistry. In: Journal of Histochemistry and Cytochemistry. 1985 ; Vol. 33, No. 6. pp. 564-658.
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