@article{a28e7ba5906b41baa8ec2a495fa25607,
title = "Segmented filamentous bacteria antigens presented by intestinal dendritic cells drive mucosal Th17 cell differentiation",
abstract = "How commensal microbiota contributes to immune cell homeostasis at barrier surfaces is poorly understood. Lamina propria (LP) T helper 17 (Th17) cells participate in mucosal protection and are induced by commensal segmented filamentous bacteria (SFB). Here we show that MHCII-dependent antigen presentation of SFB antigens by intestinal dendritic cells (DCs) is crucial for Th17 cell induction. Expression of MHCII on CD11c+ cells was necessary and sufficient for SFB-induced Th17 cell differentiation. Most SFB-induced Th17 cells recognized SFB in an MHCII-dependent manner. SFB primed and induced Th17 cells locally in the LP and Th17 cell induction occurred normally in mice lacking secondary lymphoid organs. The importance of other innate cells was unveiled by the finding that MHCII deficiency in group 3 innate lymphoid cells (ILCs) resulted in an increase in SFB-independent Th17 cell differentiation. Our results outline the complex role of DCs and ILCs in the regulation of intestinal Th17 cell homeostasis.",
author = "Yoshiyuki Goto and Casandra Panea and Gaku Nakato and Anna Cebula and Carolyn Lee and Diez, {Marta Galan} and Laufer, {Terri M.} and Leszek Ignatowicz and Ivanov, {Ivaylo I.}",
note = "Funding Information: We thank members of the I.I.I. laboratory for their help in all aspects of the project. We thank Yoshinori Umesaki at the Yakult Central Institute for providing feces from SFB-monocolonized mice. We thank Kenya Honda and Seiko Narushima for developing and sharing the SFB sorting protocol. We thank Steve Reiner and Boris Reizis for reading the manuscript and for invaluable scientific discussions. We thank Siu-Hong Ho at the Columbia Center for Translational Immunology Flow Cytometry Core and Amir Figueroa at the Department of Microbiology and Immunology Flow Cytometry Core for help with sorting. This work was supported by the National Institute of Health 1R01DK098378 to I.I.I. and by the Crohn{\textquoteright}s and Colitis Foundation of America SRA#259540 to I.I.I. G.N. was supported by a long-term research grant from Toyobo Biofoundation. I.I.I. is a Pew Scholar in the Biomedical Sciences, supported by the Pew Charitable Trust. ",
year = "2014",
month = apr,
day = "17",
doi = "10.1016/j.immuni.2014.03.005",
language = "English (US)",
volume = "40",
pages = "594--607",
journal = "Immunity",
issn = "1074-7613",
publisher = "Cell Press",
number = "4",
}
