Selective coexpression of insulin receptor-related receptor (IRR) and TRK in NGF-sensitive neurons

R. R. Reinhardt, E. Chin, B. Zhang, R. A. Roth, C. A. Bondy

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

The insulin receptor-related receptor (IRR) has recently been identified as a member of the insulin receptor tyrosine kinase family; however, its endogenous ligand and biological function are still unknown. In contrast to the very widespread pattern of expression of the homologous insulin and IGF- I receptors, IRR demonstrates a very restricted cellular distribution. Using in situ hybridization and immunohistochemistry, we now show that the expression of this receptor is selectively concentrated in a subset of neurons where its appearance is closely associated with that of the NGF receptor TRK. IRR and TRK demonstrate synchronized patterns of coexpression in neural crest-derived sensory and sympathetic neurons and in non-neural crest basal forebrain and striatal neurons. Both appear early in the embryonic development of dorsal root and trigeminal neurons and somewhat later, near the time of birth, in sympathetic neurons. Expression of both IRR and TRK appears perinatally in basal forebrain neurons, reaching maximal levels about postnatal day 20. This association is highly selective, since TRK mRNA is not detected anywhere in the developing nervous system in the absence of coordinate IRR expression, and the same is true for IRR expression with respect to TRK. In the adult rat, the majority of TRK-positive sensory neurons still express IRR mRNA, and coexpression in sympathetic and forebrain neurons continues without evidence of diminution. These findings are consistent with a functional linkage of the IRR and TRK receptors in NGF- sensitive neurons.

Original languageEnglish (US)
Pages (from-to)4674-4683
Number of pages10
JournalJournal of Neuroscience
Volume14
Issue number8
StatePublished - Aug 1 1994
Externally publishedYes

Fingerprint

Nerve Growth Factor
Neurons
Sensory Receptor Cells
Nerve Growth Factor Receptors
Corpus Striatum
Nerve Growth Factor Receptor
IGF Type 1 Receptor
Messenger RNA
insulin receptor-related receptor
Spinal Nerve Roots
Neural Crest
Prosencephalon
Nervous System
Embryonic Development
In Situ Hybridization
Immunohistochemistry
Parturition
Insulin
Ligands

Keywords

  • basal forebrain
  • embryonic development
  • neural crest
  • neurotrophin
  • sensory neuron
  • tyrosine kinase

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Selective coexpression of insulin receptor-related receptor (IRR) and TRK in NGF-sensitive neurons. / Reinhardt, R. R.; Chin, E.; Zhang, B.; Roth, R. A.; Bondy, C. A.

In: Journal of Neuroscience, Vol. 14, No. 8, 01.08.1994, p. 4674-4683.

Research output: Contribution to journalArticle

Reinhardt, RR, Chin, E, Zhang, B, Roth, RA & Bondy, CA 1994, 'Selective coexpression of insulin receptor-related receptor (IRR) and TRK in NGF-sensitive neurons', Journal of Neuroscience, vol. 14, no. 8, pp. 4674-4683.
Reinhardt, R. R. ; Chin, E. ; Zhang, B. ; Roth, R. A. ; Bondy, C. A. / Selective coexpression of insulin receptor-related receptor (IRR) and TRK in NGF-sensitive neurons. In: Journal of Neuroscience. 1994 ; Vol. 14, No. 8. pp. 4674-4683.
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