Selective recapitulation of conserved and nonconserved regions of putative noxa1 protein activation domain confers isoform-specific inhibition of nox1 oxidase and attenuation of endothelial cell migration

Daniel J. Ranayhossaini, Andres I. Rodriguez, Sanghamitra Sahoo, Beibei B. Chen, Rama K. Mallampalli, Eric E. Kelley, Gabor Csanyi, Mark T. Gladwin, Guillermo Romero, Patrick J. Pagano

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Background: Nox1, an oxidant source in colon carcinoma and vascular disease, is activated by NOXA1. Results: A NOXA1 peptide blocked NOXA1-Nox1 binding and inhibited colon carcinoma and endothelial oxidants and migration. Conclusion: The findings identify a NOXA1-Activating domain and an isoform-specific Nox1 inhibitor. Significance: The data provide insight into Nox1 regulation and present a potential therapy for suppressing oxidative stressrelated disease.

Original languageEnglish (US)
Pages (from-to)36437-36450
Number of pages14
JournalJournal of Biological Chemistry
Volume288
Issue number51
DOIs
StatePublished - Dec 20 2013

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Endothelial cells
Oxidants
Cell Movement
Oxidoreductases
Protein Isoforms
Colon
Endothelial Cells
Chemical activation
Carcinoma
Vascular Diseases
Proteins
Peptides
Protein Domains
Therapeutics

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Selective recapitulation of conserved and nonconserved regions of putative noxa1 protein activation domain confers isoform-specific inhibition of nox1 oxidase and attenuation of endothelial cell migration. / Ranayhossaini, Daniel J.; Rodriguez, Andres I.; Sahoo, Sanghamitra; Chen, Beibei B.; Mallampalli, Rama K.; Kelley, Eric E.; Csanyi, Gabor; Gladwin, Mark T.; Romero, Guillermo; Pagano, Patrick J.

In: Journal of Biological Chemistry, Vol. 288, No. 51, 20.12.2013, p. 36437-36450.

Research output: Contribution to journalArticle

Ranayhossaini, Daniel J. ; Rodriguez, Andres I. ; Sahoo, Sanghamitra ; Chen, Beibei B. ; Mallampalli, Rama K. ; Kelley, Eric E. ; Csanyi, Gabor ; Gladwin, Mark T. ; Romero, Guillermo ; Pagano, Patrick J. / Selective recapitulation of conserved and nonconserved regions of putative noxa1 protein activation domain confers isoform-specific inhibition of nox1 oxidase and attenuation of endothelial cell migration. In: Journal of Biological Chemistry. 2013 ; Vol. 288, No. 51. pp. 36437-36450.
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abstract = "Background: Nox1, an oxidant source in colon carcinoma and vascular disease, is activated by NOXA1. Results: A NOXA1 peptide blocked NOXA1-Nox1 binding and inhibited colon carcinoma and endothelial oxidants and migration. Conclusion: The findings identify a NOXA1-Activating domain and an isoform-specific Nox1 inhibitor. Significance: The data provide insight into Nox1 regulation and present a potential therapy for suppressing oxidative stressrelated disease.",
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AU - Sahoo, Sanghamitra

AU - Chen, Beibei B.

AU - Mallampalli, Rama K.

AU - Kelley, Eric E.

AU - Csanyi, Gabor

AU - Gladwin, Mark T.

AU - Romero, Guillermo

AU - Pagano, Patrick J.

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