Selenoprotein expression is essential in endothelial cell development and cardiac muscle function

Rajeev Kumar Shrimali, James A. Weaver, Georgina F. Miller, Matthew F. Starost, Bradley A. Carlson, Sergey V. Novoselov, Easwari Kumaraswamy, Vadim N. Gladyshev, Dolph L. Hatfield

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

LoxP-Cre technology was used to remove the selenocysteine tRNA gene, trsp, in either endothelial cells or myocytes of skeletal and heart muscle to elucidate the role of selenoproteins in cardiovascular disease. Loss of selenoprotein expression in endothelial cells was embryonic lethal. A 14.5-day-old embryo had numerous abnormalities including necrosis of the central nervous system, subcutaneous hemorrhage and erythrocyte immaturity. Loss of selenoprotein expression in myocytes manifested no apparent phenotype until about day 12 after birth. Affected mice had decreased mobility and an increased respiratory rate, which proceeded rapidly to death. Pathological analysis revealed that mice lacking trsp had moderate to severe myocarditis with inflammation extending into the mediastinitis. Thus, ablation of selenoprotein expression demonstrated an essential role of selenoproteins in endothelial cell development and in proper cardiac muscle function. The data suggest a direct connection between the loss of selenoprotein expression in these cell types and cardiovascular disease.

Original languageEnglish (US)
Pages (from-to)135-142
Number of pages8
JournalNeuromuscular Disorders
Volume17
Issue number2
DOIs
StatePublished - Feb 1 2007

Fingerprint

Selenoproteins
Myocardium
Endothelial Cells
Muscle Cells
Cardiovascular Diseases
Mediastinitis
Myocarditis
Respiratory Rate
Skeletal Muscle
Necrosis
Embryonic Structures
Central Nervous System
Erythrocytes
Parturition
Hemorrhage
Inflammation
Technology
Phenotype
Genes

Keywords

  • Cardiovascular disease
  • Conditional knockout
  • Endothelial cells
  • Heart muscle
  • Selenocysteine
  • Selenocysteine tRNA
  • Selenoproteins

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health
  • Neurology
  • Clinical Neurology
  • Genetics(clinical)

Cite this

Shrimali, R. K., Weaver, J. A., Miller, G. F., Starost, M. F., Carlson, B. A., Novoselov, S. V., ... Hatfield, D. L. (2007). Selenoprotein expression is essential in endothelial cell development and cardiac muscle function. Neuromuscular Disorders, 17(2), 135-142. https://doi.org/10.1016/j.nmd.2006.10.006

Selenoprotein expression is essential in endothelial cell development and cardiac muscle function. / Shrimali, Rajeev Kumar; Weaver, James A.; Miller, Georgina F.; Starost, Matthew F.; Carlson, Bradley A.; Novoselov, Sergey V.; Kumaraswamy, Easwari; Gladyshev, Vadim N.; Hatfield, Dolph L.

In: Neuromuscular Disorders, Vol. 17, No. 2, 01.02.2007, p. 135-142.

Research output: Contribution to journalArticle

Shrimali, RK, Weaver, JA, Miller, GF, Starost, MF, Carlson, BA, Novoselov, SV, Kumaraswamy, E, Gladyshev, VN & Hatfield, DL 2007, 'Selenoprotein expression is essential in endothelial cell development and cardiac muscle function', Neuromuscular Disorders, vol. 17, no. 2, pp. 135-142. https://doi.org/10.1016/j.nmd.2006.10.006
Shrimali RK, Weaver JA, Miller GF, Starost MF, Carlson BA, Novoselov SV et al. Selenoprotein expression is essential in endothelial cell development and cardiac muscle function. Neuromuscular Disorders. 2007 Feb 1;17(2):135-142. https://doi.org/10.1016/j.nmd.2006.10.006
Shrimali, Rajeev Kumar ; Weaver, James A. ; Miller, Georgina F. ; Starost, Matthew F. ; Carlson, Bradley A. ; Novoselov, Sergey V. ; Kumaraswamy, Easwari ; Gladyshev, Vadim N. ; Hatfield, Dolph L. / Selenoprotein expression is essential in endothelial cell development and cardiac muscle function. In: Neuromuscular Disorders. 2007 ; Vol. 17, No. 2. pp. 135-142.
@article{da0c74091e144156b35c901a4b846735,
title = "Selenoprotein expression is essential in endothelial cell development and cardiac muscle function",
abstract = "LoxP-Cre technology was used to remove the selenocysteine tRNA gene, trsp, in either endothelial cells or myocytes of skeletal and heart muscle to elucidate the role of selenoproteins in cardiovascular disease. Loss of selenoprotein expression in endothelial cells was embryonic lethal. A 14.5-day-old embryo had numerous abnormalities including necrosis of the central nervous system, subcutaneous hemorrhage and erythrocyte immaturity. Loss of selenoprotein expression in myocytes manifested no apparent phenotype until about day 12 after birth. Affected mice had decreased mobility and an increased respiratory rate, which proceeded rapidly to death. Pathological analysis revealed that mice lacking trsp had moderate to severe myocarditis with inflammation extending into the mediastinitis. Thus, ablation of selenoprotein expression demonstrated an essential role of selenoproteins in endothelial cell development and in proper cardiac muscle function. The data suggest a direct connection between the loss of selenoprotein expression in these cell types and cardiovascular disease.",
keywords = "Cardiovascular disease, Conditional knockout, Endothelial cells, Heart muscle, Selenocysteine, Selenocysteine tRNA, Selenoproteins",
author = "Shrimali, {Rajeev Kumar} and Weaver, {James A.} and Miller, {Georgina F.} and Starost, {Matthew F.} and Carlson, {Bradley A.} and Novoselov, {Sergey V.} and Easwari Kumaraswamy and Gladyshev, {Vadim N.} and Hatfield, {Dolph L.}",
year = "2007",
month = "2",
day = "1",
doi = "10.1016/j.nmd.2006.10.006",
language = "English (US)",
volume = "17",
pages = "135--142",
journal = "Neuromuscular Disorders",
issn = "0960-8966",
publisher = "Elsevier Limited",
number = "2",

}

TY - JOUR

T1 - Selenoprotein expression is essential in endothelial cell development and cardiac muscle function

AU - Shrimali, Rajeev Kumar

AU - Weaver, James A.

AU - Miller, Georgina F.

AU - Starost, Matthew F.

AU - Carlson, Bradley A.

AU - Novoselov, Sergey V.

AU - Kumaraswamy, Easwari

AU - Gladyshev, Vadim N.

AU - Hatfield, Dolph L.

PY - 2007/2/1

Y1 - 2007/2/1

N2 - LoxP-Cre technology was used to remove the selenocysteine tRNA gene, trsp, in either endothelial cells or myocytes of skeletal and heart muscle to elucidate the role of selenoproteins in cardiovascular disease. Loss of selenoprotein expression in endothelial cells was embryonic lethal. A 14.5-day-old embryo had numerous abnormalities including necrosis of the central nervous system, subcutaneous hemorrhage and erythrocyte immaturity. Loss of selenoprotein expression in myocytes manifested no apparent phenotype until about day 12 after birth. Affected mice had decreased mobility and an increased respiratory rate, which proceeded rapidly to death. Pathological analysis revealed that mice lacking trsp had moderate to severe myocarditis with inflammation extending into the mediastinitis. Thus, ablation of selenoprotein expression demonstrated an essential role of selenoproteins in endothelial cell development and in proper cardiac muscle function. The data suggest a direct connection between the loss of selenoprotein expression in these cell types and cardiovascular disease.

AB - LoxP-Cre technology was used to remove the selenocysteine tRNA gene, trsp, in either endothelial cells or myocytes of skeletal and heart muscle to elucidate the role of selenoproteins in cardiovascular disease. Loss of selenoprotein expression in endothelial cells was embryonic lethal. A 14.5-day-old embryo had numerous abnormalities including necrosis of the central nervous system, subcutaneous hemorrhage and erythrocyte immaturity. Loss of selenoprotein expression in myocytes manifested no apparent phenotype until about day 12 after birth. Affected mice had decreased mobility and an increased respiratory rate, which proceeded rapidly to death. Pathological analysis revealed that mice lacking trsp had moderate to severe myocarditis with inflammation extending into the mediastinitis. Thus, ablation of selenoprotein expression demonstrated an essential role of selenoproteins in endothelial cell development and in proper cardiac muscle function. The data suggest a direct connection between the loss of selenoprotein expression in these cell types and cardiovascular disease.

KW - Cardiovascular disease

KW - Conditional knockout

KW - Endothelial cells

KW - Heart muscle

KW - Selenocysteine

KW - Selenocysteine tRNA

KW - Selenoproteins

UR - http://www.scopus.com/inward/record.url?scp=33847191027&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33847191027&partnerID=8YFLogxK

U2 - 10.1016/j.nmd.2006.10.006

DO - 10.1016/j.nmd.2006.10.006

M3 - Article

VL - 17

SP - 135

EP - 142

JO - Neuromuscular Disorders

JF - Neuromuscular Disorders

SN - 0960-8966

IS - 2

ER -