Selenoproteins mediate T cell immunity through an antioxidant mechanism

Rajeev Kumar Shrimali, Robert D. Irons, Bradley A. Carlson, Yasuyo Sano, Vadim N. Gladyshev, Mo Park Jin, Dolph L. Hatfield

Research output: Contribution to journalArticle

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Abstract

Selenium is an essential dietary element with antioxidant roles in immune regulation, but there is little understanding of how this element acts at the molecular level in host defense and inflammatory disease. Selenium is incorporated into the amino acid selenocysteine (Sec), which in turn is inserted into seleno-proteins in a manner dependent on Sec tRNA[Ser]Sec. To investigate the molecular mechanism that links selenium to T cell immunity, we generated mice with selenoprotein-less T cells by cell type-specific ablation of the Sec tRNA[Ser]Sec gene (trsp). Herein, we show that these mutant mice exhibit decreased pools of mature T cells and a defect in T cell-dependent antibody responses. We also demonstrate that selenoprotein deficiency leads to oxidant hyperproduction in T cells and thereby suppresses T cell proliferation in response to T cell receptor stimulation. These findings offer novel insights into immune function of selenium and physiological antioxidants.

Original languageEnglish (US)
Pages (from-to)20181-20185
Number of pages5
JournalJournal of Biological Chemistry
Volume283
Issue number29
DOIs
StatePublished - Jul 18 2008

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Selenoproteins
T-cells
Immunity
Antioxidants
Selenocysteine
Selenium
T-Lymphocytes
Cell proliferation
T-Cell Antigen Receptor
Ablation
Oxidants
Antibody Formation
Genes
Cell Proliferation
Amino Acids
Defects
Antibodies

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Shrimali, R. K., Irons, R. D., Carlson, B. A., Sano, Y., Gladyshev, V. N., Jin, M. P., & Hatfield, D. L. (2008). Selenoproteins mediate T cell immunity through an antioxidant mechanism. Journal of Biological Chemistry, 283(29), 20181-20185. https://doi.org/10.1074/jbc.M802559200

Selenoproteins mediate T cell immunity through an antioxidant mechanism. / Shrimali, Rajeev Kumar; Irons, Robert D.; Carlson, Bradley A.; Sano, Yasuyo; Gladyshev, Vadim N.; Jin, Mo Park; Hatfield, Dolph L.

In: Journal of Biological Chemistry, Vol. 283, No. 29, 18.07.2008, p. 20181-20185.

Research output: Contribution to journalArticle

Shrimali, RK, Irons, RD, Carlson, BA, Sano, Y, Gladyshev, VN, Jin, MP & Hatfield, DL 2008, 'Selenoproteins mediate T cell immunity through an antioxidant mechanism', Journal of Biological Chemistry, vol. 283, no. 29, pp. 20181-20185. https://doi.org/10.1074/jbc.M802559200
Shrimali RK, Irons RD, Carlson BA, Sano Y, Gladyshev VN, Jin MP et al. Selenoproteins mediate T cell immunity through an antioxidant mechanism. Journal of Biological Chemistry. 2008 Jul 18;283(29):20181-20185. https://doi.org/10.1074/jbc.M802559200
Shrimali, Rajeev Kumar ; Irons, Robert D. ; Carlson, Bradley A. ; Sano, Yasuyo ; Gladyshev, Vadim N. ; Jin, Mo Park ; Hatfield, Dolph L. / Selenoproteins mediate T cell immunity through an antioxidant mechanism. In: Journal of Biological Chemistry. 2008 ; Vol. 283, No. 29. pp. 20181-20185.
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