Semaphorin 3A Is a New Early Diagnostic Biomarker of Experimental and Pediatric Acute Kidney Injury

Calpurnia Jayakumar, Punithavathi Ranganathan, Prasad Devarajan, Catherine D. Krawczeski, Stephen Warwick Looney, Ganesan Ramesh

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Background: Semaphorin 3A is a secreted protein that regulates cell motility and attachment in axon guidance, vascular growth, immune cell regulation and tumor progression. However, nothing is known about its role in kidney pathophysiology. Here, we determined whether semaphorin3A is induced after acute kidney injury (AKI) and whether urinary semaphorin 3A can predict AKI in humans undergoing cardiopulmonary bypass (CPB). Methods and Principal Findings: In animals, semaphorin 3A is localized in distal tubules of the kidney and excretion increased within 3 hr after reperfusion of the kidney whereas serum creatinine was significantly raised at 24 hr. In humans, using serum creatinine, AKI was detected on average only 48 hours after CPB. In contrast, urine semaphorin increased at 2 hours after CPB, peaked at 6 hours (2596±591 pg/mg creatinine), and was no longer significantly elevated 12 hours after CPB. The predictive power of semaphorin 3A as demonstrated by area under the receiver-operating characteristic curve for diagnosis of AKI at 2, 6, and 12 hours after CPB was 0.88, 0.81, and 0.74, respectively. The 2-hour urine semaphorin measurement strongly correlated with duration and severity of AKI, as well as length of hospital stay. Adjusting for CPB time and gender, the 2-hour semaphorin remained an independent predictor of AKI, with an odds ratio of 2.19. Conclusion: Our results suggest that semaphorin 3A is an early, predictive biomarker in experimental and pediatric AKI, and may allow for the reliable early diagnosis and prognosis of AKI after CPB, much before the rise in serum creatinine.

Original languageEnglish (US)
Article numbere58446
JournalPloS one
Volume8
Issue number3
DOIs
StatePublished - Mar 4 2013

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Semaphorin-3A
Pediatrics
Biomarkers
Acute Kidney Injury
Cardiopulmonary Bypass
Semaphorins
biomarkers
kidneys
Creatinine
creatinine
Length of Stay
Distal Kidney Tubule
Serum
Urine
Tumors
Kidney
Animals
urine
renal tubules
ROC Curve

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Semaphorin 3A Is a New Early Diagnostic Biomarker of Experimental and Pediatric Acute Kidney Injury. / Jayakumar, Calpurnia; Ranganathan, Punithavathi; Devarajan, Prasad; Krawczeski, Catherine D.; Looney, Stephen Warwick; Ramesh, Ganesan.

In: PloS one, Vol. 8, No. 3, e58446, 04.03.2013.

Research output: Contribution to journalArticle

Jayakumar, Calpurnia ; Ranganathan, Punithavathi ; Devarajan, Prasad ; Krawczeski, Catherine D. ; Looney, Stephen Warwick ; Ramesh, Ganesan. / Semaphorin 3A Is a New Early Diagnostic Biomarker of Experimental and Pediatric Acute Kidney Injury. In: PloS one. 2013 ; Vol. 8, No. 3.
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