Semen analysis data from fresh and cryopreserved donor ejaculates

Comparison of cryoprotectants and pregnancy rates

Brooks Allen Keel, B. W. Webster

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Patients (155) were selected at random for fresh or cryopreserved semen and inseminated on the predicted day of ovulation. Semen analysis was performed using a microcomputerized multiple-exposure photography system. Frozen semen was used with either glycerol or TEST-yolk (TEST-buffered 20% egg yolk with 10% glycerol) as the cryoprotectant. Cryopreservation resulted in significant decreases in all semen parameters measured. Of these, velocity appeared to be the least effected. TEST-yolk provided significantly more protection against a reduction in velocity compared with glycerol. A total of 18, 17, and 27 patients conceived using fresh, glycerol, or TEST-yolk-preserved semen, repectively. For these same groups, a cumulative pregnancy rate of 52.9%, 27.1%, and 68.5%, respectively, was observed (not significant). The total number of motile sperm per insemination used for fresh artificial inseminations resulting in conception (132.4 x 106) was significantly greater than the number used for successful glycerol- and TEST-yolk-preserved serum (approximately 24 x 106). These results demonstrate that although the number of motile sperm of cryopreserved ejaculates are dramatically reduced compared with the fresh counterparts, if a mimimum criteria for ejaculate quality is established, the use of cryopreserved semen can offer a viable, effective, and relatively safe alternative to artificial insemination by donor with fresh semen.

Original languageEnglish (US)
Pages (from-to)100-105
Number of pages6
JournalFertility and Sterility
Volume52
Issue number1
DOIs
StatePublished - Jan 1 1989
Externally publishedYes

Fingerprint

Semen Analysis
Pregnancy Rate
Semen
Glycerol
Tissue Donors
Sperm Count
Semen Preservation
Artificial Insemination
Egg Yolk
Insemination
Photography
Cryopreservation
Ovulation
Serum

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynecology

Cite this

Semen analysis data from fresh and cryopreserved donor ejaculates : Comparison of cryoprotectants and pregnancy rates. / Keel, Brooks Allen; Webster, B. W.

In: Fertility and Sterility, Vol. 52, No. 1, 01.01.1989, p. 100-105.

Research output: Contribution to journalArticle

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