Sensitive liquid chromatography/tandem mass spectrometry method for the determination of two novel highly lipophilic anticancer drug candidates in rat plasma and tissues

Shirin Hooshfar, Michael R. Linzey, Daqing Wu, Lajos Gera, Kenza Mamouni, Xin Li, Yanhua Chen, Yang Yang, Oluwasegun Olorunyolemi, Michael G. Bartlett

Research output: Contribution to journalArticle

Abstract

A simple and sensitive liquid chromatography/electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) method was developed and validated for determination of two highly lipophilic anticancer drug candidates, LG1980 and GH501, in rat plasma and tissues (liver, kidney and femur bones). LG1980 and GH501 were extracted from rat plasma and tissue homogenates using liquid–liquid extraction. The method provided a linear range of 1.0–200.0 ng/mL for GH501 in plasma and LG1980 in plasma and liver. For both analytes in other tissue homogenates the linear range was 2.0–400.0 ng/mL. The method was validated with precision within 15% relative standard deviation, accuracy within 15% relative error and a consistent recovery. This method has been successfully applied in two preclinical studies for LG1980 and GH501 to determine their concentrations in rat plasma, liver, kidney and bone over 24 h after intravenous injection of compounds.

Original languageEnglish (US)
Article numbere4064
JournalBiomedical Chromatography
Volume32
Issue number2
DOIs
StatePublished - Feb 1 2018

Fingerprint

Liquid chromatography
Tandem Mass Spectrometry
Liquid Chromatography
Mass spectrometry
Rats
Tissue
Plasmas
Liver
Pharmaceutical Preparations
Bone
Kidney
Bone and Bones
Electrospray ionization
Electrospray Ionization Mass Spectrometry
Intravenous Injections
Femur
Recovery

Keywords

  • LC–MS/MS
  • bioanalysis
  • highly lipophilic drug
  • plasma
  • tissue

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Molecular Biology
  • Pharmacology
  • Drug Discovery
  • Clinical Biochemistry

Cite this

Sensitive liquid chromatography/tandem mass spectrometry method for the determination of two novel highly lipophilic anticancer drug candidates in rat plasma and tissues. / Hooshfar, Shirin; Linzey, Michael R.; Wu, Daqing; Gera, Lajos; Mamouni, Kenza; Li, Xin; Chen, Yanhua; Yang, Yang; Olorunyolemi, Oluwasegun; Bartlett, Michael G.

In: Biomedical Chromatography, Vol. 32, No. 2, e4064, 01.02.2018.

Research output: Contribution to journalArticle

Hooshfar, Shirin ; Linzey, Michael R. ; Wu, Daqing ; Gera, Lajos ; Mamouni, Kenza ; Li, Xin ; Chen, Yanhua ; Yang, Yang ; Olorunyolemi, Oluwasegun ; Bartlett, Michael G. / Sensitive liquid chromatography/tandem mass spectrometry method for the determination of two novel highly lipophilic anticancer drug candidates in rat plasma and tissues. In: Biomedical Chromatography. 2018 ; Vol. 32, No. 2.
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abstract = "A simple and sensitive liquid chromatography/electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) method was developed and validated for determination of two highly lipophilic anticancer drug candidates, LG1980 and GH501, in rat plasma and tissues (liver, kidney and femur bones). LG1980 and GH501 were extracted from rat plasma and tissue homogenates using liquid–liquid extraction. The method provided a linear range of 1.0–200.0 ng/mL for GH501 in plasma and LG1980 in plasma and liver. For both analytes in other tissue homogenates the linear range was 2.0–400.0 ng/mL. The method was validated with precision within 15{\%} relative standard deviation, accuracy within 15{\%} relative error and a consistent recovery. This method has been successfully applied in two preclinical studies for LG1980 and GH501 to determine their concentrations in rat plasma, liver, kidney and bone over 24 h after intravenous injection of compounds.",
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AU - Li, Xin

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