Sensitivity of ob/ob mice to Leptin-induced adipose tissue apoptosis

Mary Anne Della-Fera, Yang Ho Choi, Diane L. Hartzell, Juihua Duan, Mark W Hamrick, Clifton A. Baile

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Objective: ob/ob mice have increased sensitivity to many of leptin's effects. The primary objective of this experiment was to determine whether ob/ob mice demonstrated increased sensitivity to leptin-induced adipose tissue apoptosis. Research Methods and Procedures: Fifteen-week-old female ob/ob and Ob/? mice received 0 (saline), 2.5. or 10 μg/d leptin for 14 days through subcutaneous (sc) osmotic minipumps. Food intake (FI), body temperature, physical activity, and body weight were measured daily. Body composition and weights and adipose tissue apoptosis (percentage DNA fragmentation) of inguinal, parametrial. and retroperitoneal fat pads were determined at the end of the study. Results: FI decreases were more pronounced in ob/ob. Leptin (10 μg/d) decreased total FI 71% in ob/ob and 34% in Ob/? (p < 0.05). Body weight was decreased by both doses of leptin in ob/ob (p < 0.01) but was unchanged in Ob/?. Leptin increased body temperature in ob/ob but not in Ob/?. Physical activity was increased 400% by 10 μg/d leptin in ob/ob (p < 0.01) but decreased 13% in Ob/? (p < 0.01). Body fat content of ob/ob was reduced by both leptin doses, whereas only 10 μg/d leptin decreased body fat in Ob/?. Fat pad weights were decreased similarly by leptin in both genotypes. However, apoptosis was increased by leptin in all three fat pads in ob/ob, whereas Ob/? showed significant increases only in retroperitoneal. Discussion: ob/ob mice had greater overall sensitivity to leptin. Although ob/ob mice appeared to be more sensitive than Ob/? mice to leptin-induced adipose tissue apoptosis. there were differences among adipose depots in responsiveness to leptin-induced apoptosis.

Original languageEnglish (US)
Pages (from-to)1540-1547
Number of pages8
JournalObesity Research
Volume13
Issue number9
DOIs
StatePublished - Jan 1 2005

Fingerprint

Leptin
leptin
adipose tissue
Adipose Tissue
apoptosis
Apoptosis
mice
food intake
Eating
Body Weight
Body Temperature
physical activity
body temperature
body fat
body weight
lipids
Intra-Abdominal Fat
Groin
DNA fragmentation
DNA Fragmentation

Keywords

  • Adipose tissue
  • Apoptosis
  • Leptin
  • Ob/ob mice

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Food Science
  • Endocrinology, Diabetes and Metabolism
  • Endocrinology
  • Public Health, Environmental and Occupational Health

Cite this

Della-Fera, M. A., Choi, Y. H., Hartzell, D. L., Duan, J., Hamrick, M. W., & Baile, C. A. (2005). Sensitivity of ob/ob mice to Leptin-induced adipose tissue apoptosis. Obesity Research, 13(9), 1540-1547. https://doi.org/10.1038/oby.2005.189

Sensitivity of ob/ob mice to Leptin-induced adipose tissue apoptosis. / Della-Fera, Mary Anne; Choi, Yang Ho; Hartzell, Diane L.; Duan, Juihua; Hamrick, Mark W; Baile, Clifton A.

In: Obesity Research, Vol. 13, No. 9, 01.01.2005, p. 1540-1547.

Research output: Contribution to journalArticle

Della-Fera, MA, Choi, YH, Hartzell, DL, Duan, J, Hamrick, MW & Baile, CA 2005, 'Sensitivity of ob/ob mice to Leptin-induced adipose tissue apoptosis', Obesity Research, vol. 13, no. 9, pp. 1540-1547. https://doi.org/10.1038/oby.2005.189
Della-Fera, Mary Anne ; Choi, Yang Ho ; Hartzell, Diane L. ; Duan, Juihua ; Hamrick, Mark W ; Baile, Clifton A. / Sensitivity of ob/ob mice to Leptin-induced adipose tissue apoptosis. In: Obesity Research. 2005 ; Vol. 13, No. 9. pp. 1540-1547.
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abstract = "Objective: ob/ob mice have increased sensitivity to many of leptin's effects. The primary objective of this experiment was to determine whether ob/ob mice demonstrated increased sensitivity to leptin-induced adipose tissue apoptosis. Research Methods and Procedures: Fifteen-week-old female ob/ob and Ob/? mice received 0 (saline), 2.5. or 10 μg/d leptin for 14 days through subcutaneous (sc) osmotic minipumps. Food intake (FI), body temperature, physical activity, and body weight were measured daily. Body composition and weights and adipose tissue apoptosis (percentage DNA fragmentation) of inguinal, parametrial. and retroperitoneal fat pads were determined at the end of the study. Results: FI decreases were more pronounced in ob/ob. Leptin (10 μg/d) decreased total FI 71{\%} in ob/ob and 34{\%} in Ob/? (p < 0.05). Body weight was decreased by both doses of leptin in ob/ob (p < 0.01) but was unchanged in Ob/?. Leptin increased body temperature in ob/ob but not in Ob/?. Physical activity was increased 400{\%} by 10 μg/d leptin in ob/ob (p < 0.01) but decreased 13{\%} in Ob/? (p < 0.01). Body fat content of ob/ob was reduced by both leptin doses, whereas only 10 μg/d leptin decreased body fat in Ob/?. Fat pad weights were decreased similarly by leptin in both genotypes. However, apoptosis was increased by leptin in all three fat pads in ob/ob, whereas Ob/? showed significant increases only in retroperitoneal. Discussion: ob/ob mice had greater overall sensitivity to leptin. Although ob/ob mice appeared to be more sensitive than Ob/? mice to leptin-induced adipose tissue apoptosis. there were differences among adipose depots in responsiveness to leptin-induced apoptosis.",
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