Sensitization of Lewis rats with sulfoglucuronosyl paragloboside

Electrophysiological and immunological studies of an animal model of peripheral neuropathy

M. Yamawaki, A. Vasquez, A. Ben Younes, H. Yoshino, T. Kanda, T. Ariga, N. Baumann, Robert K Yu

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Antibodies against sulfoglucuronosyl glycosphingolipids (SGGLs) are known to be present in sera of patients with chronic polyneuropathy associated with IgM paraproteinemia. We recently studied rats sensitized with sulfoglucuronosyl paragloboside (SGPG), a major SGGL species, emulsified with keyhold limpet hemocyanin and Freund's adjuvant. The titer of the IgM class antibodies against SGPG increased up to 1:1,600, while that of the IgG class increased up to 1:800 2 weeks after sensitization. The antibodies showed a high degree of antigenic specificity; no cross-reactivity with other brain glycolipids could be detected. They, however, reacted with human myelin- associated glycoprotein (MAG) by Western blot analysis, but not with rat MAG. These animal models showed minor but clear clinical signs of neuropathy, consisting of mild tail muscle tone loss and walking disabilities. Electrophysiological examination of the sciatic nerves revealed nerve conduction abnormalities which consisted of conduction block and mild decrease in conduction velocity. Thus, our results support the concept that anti-SGPG antibodies may play an important pathogenetic role in this type of chronic neuropathy.

Original languageEnglish (US)
Pages (from-to)58-65
Number of pages8
JournalJournal of Neuroscience Research
Volume44
Issue number1
DOIs
StatePublished - Apr 22 1996

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Peripheral Nervous System Diseases
Glycosphingolipids
Animal Models
Immunoglobulin M
Antibodies
Myelin-Associated Glycoprotein
Hemocyanin
Paraproteinemias
Freund's Adjuvant
Polyneuropathies
Immunoglobulin Isotypes
Glycolipids
Neural Conduction
Sciatic Nerve
Walking
Tail
Epitopes
Immunoglobulin G
Western Blotting
Muscles

Keywords

  • MAG
  • SGGLs
  • SGPG
  • neuropathy

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

Cite this

Sensitization of Lewis rats with sulfoglucuronosyl paragloboside : Electrophysiological and immunological studies of an animal model of peripheral neuropathy. / Yamawaki, M.; Vasquez, A.; Younes, A. Ben; Yoshino, H.; Kanda, T.; Ariga, T.; Baumann, N.; Yu, Robert K.

In: Journal of Neuroscience Research, Vol. 44, No. 1, 22.04.1996, p. 58-65.

Research output: Contribution to journalArticle

Yamawaki, M. ; Vasquez, A. ; Younes, A. Ben ; Yoshino, H. ; Kanda, T. ; Ariga, T. ; Baumann, N. ; Yu, Robert K. / Sensitization of Lewis rats with sulfoglucuronosyl paragloboside : Electrophysiological and immunological studies of an animal model of peripheral neuropathy. In: Journal of Neuroscience Research. 1996 ; Vol. 44, No. 1. pp. 58-65.
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