Separation of chromosomal length DNA molecules

Pneumatic apparatus for rotating gels during electrophoresis

John C. Sutherland, Ann B. Emrick, John Trunk

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The maximum length of DNA molecules that can be separated by gel electrophoresis can be increased greatly by periodically altering the direction of the electric field with respect to the gel by an angle that exceeds 90°. One method involves rotating the gel by the desired angle in alternate directions periodically during electrophoresis. We describe a modification of the rotating gel electrophoresis apparatus developed by Serwer (Electrophoresis 1987, 8, 301–304) that uses a pneumatic rotary actuator instead of a stepping motor, hence reducing the cost by about 50 %. Other advantages of our design are a lower center of gravity that makes the apparatus more stable and the removal of all electrical power from beneath the fluid‐filled electrophoresis chamber. We present data demonstrating the separation of chromosomal length DNA molecules from Saccharomyces cerevisiae strain 334 into 14 resolved bands in parallel lanes.

Original languageEnglish (US)
Pages (from-to)315-317
Number of pages3
JournalElectrophoresis
Volume10
Issue number5-6
DOIs
StatePublished - Jan 1 1989
Externally publishedYes

Fingerprint

Electrophoresis
Pneumatics
Gels
Molecules
DNA
Stepping motors
Gravitation
Yeast
Saccharomyces cerevisiae
Actuators
Electric fields
Costs and Cost Analysis
Costs
Direction compound

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry

Cite this

Separation of chromosomal length DNA molecules : Pneumatic apparatus for rotating gels during electrophoresis. / Sutherland, John C.; Emrick, Ann B.; Trunk, John.

In: Electrophoresis, Vol. 10, No. 5-6, 01.01.1989, p. 315-317.

Research output: Contribution to journalArticle

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