Separation of normal and abnormal hemoglobin chains by reversed-phase high-performance liquid chromatography

Ferdane Kutlar, Abdullah Kutlar, T. H J Huisman

Research output: Contribution to journalArticle

151 Citations (Scopus)

Abstract

A review is presented of the elution patterns on reversed-phase columns of the normal and abnormal globin chains of different hemoglobin types, including 16 β-chain variants, 7 α-chain variants, 9 γ-chain variants, and 4-variants with fusion or hybrid chains. Separations appear to be based primarily on differences in hydrophobicity. The method is ideally suited for the detection of abnormal globin chains, their quantitation and their isolation. Semi-quantitative data based on the calculation of the δ non-α ratios allow the detection of β-thalassemic conditions in situations where the quantitation of hemoglobin A2 by other procedures is impossible or complicated.

Original languageEnglish (US)
Pages (from-to)147-153
Number of pages7
JournalJournal of Chromatography A
Volume357
Issue numberC
DOIs
StatePublished - Jan 1 1986

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Abnormal Hemoglobins
Globins
High performance liquid chromatography
Reverse-Phase Chromatography
Hemoglobin A2
High Pressure Liquid Chromatography
Hydrophobicity
Hydrophobic and Hydrophilic Interactions
Hemoglobins
Fusion reactions

ASJC Scopus subject areas

  • Analytical Chemistry
  • Clinical Biochemistry
  • Molecular Medicine

Cite this

Separation of normal and abnormal hemoglobin chains by reversed-phase high-performance liquid chromatography. / Kutlar, Ferdane; Kutlar, Abdullah; Huisman, T. H J.

In: Journal of Chromatography A, Vol. 357, No. C, 01.01.1986, p. 147-153.

Research output: Contribution to journalArticle

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