Separation of normal and abnormal hemoglobin chains by reversed-phase high-performance liquid chromatography

Ferdane Kutlar, Abdullah Kutlar, T. H J Huisman

Research output: Contribution to journalArticle

151 Scopus citations

Abstract

A review is presented of the elution patterns on reversed-phase columns of the normal and abnormal globin chains of different hemoglobin types, including 16 β-chain variants, 7 α-chain variants, 9 γ-chain variants, and 4-variants with fusion or hybrid chains. Separations appear to be based primarily on differences in hydrophobicity. The method is ideally suited for the detection of abnormal globin chains, their quantitation and their isolation. Semi-quantitative data based on the calculation of the δ non-α ratios allow the detection of β-thalassemic conditions in situations where the quantitation of hemoglobin A2 by other procedures is impossible or complicated.

Original languageEnglish (US)
Pages (from-to)147-153
Number of pages7
JournalJournal of Chromatography A
Volume357
Issue numberC
DOIs
Publication statusPublished - Jan 1 1986

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ASJC Scopus subject areas

  • Analytical Chemistry
  • Clinical Biochemistry
  • Molecular Medicine

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