Separation of thymic education from antigen presenting functions of major histocompatibility complex class I molecules

E. Simpson, P. J. Robinson, P. Chandler, M. M. Millrain, H. P. Pircher, D. Brandle, P. Tomlinson, J. Antoniou, A. Mellor

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Abstract

Participation of transmembrane (TM) and glycosyl-phosphatidylinositol (GPI) anchored H-2Db molecules in antigen presentation and thymic selection events was investigated using transgenic mice. Both GPI-Db and TM-Db can efficiently present H-Y antigen, influenza and lymphocytic choriomeningitis virus (LCMV) peptides to primed cytotoxic. H-2Db-restricted T cells. Transgenic mice expressing GPI-Db, although unable to reject TM-Db skin grafts, nevertheless generate secondary CTL responses which can lyse TM-Db- bearing targets, indicating that GPI-Db mice fail to delete all TM-Db- reactive T cells. Furthermore, double-transgenic mice bearing GPI-Db and a T-cell receptor (TcR) for H-2Db + LCMV do not positively select receptor positive, CD8+CD4- T cells. This paradoxical behaviour of GPI-Db molecules suggests that the structural requirements for antigen presentation and thymic selection by class I molecules are different and may explain why GPI-linked class I molecules, such as Qa-2, do not appear to function as restriction elements in vivo.

Original languageEnglish (US)
Pages (from-to)132-136
Number of pages5
JournalImmunology
Volume81
Issue number1
StatePublished - Jan 1 1994

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Glycosylphosphatidylinositols
Major Histocompatibility Complex
Education
Antigens
Transgenic Mice
Lymphocytic choriomeningitis virus
Antigen Presentation
T-Lymphocytes
H-Y Antigen
T-Cell Antigen Receptor
Human Influenza
Transplants
Skin
Peptides

ASJC Scopus subject areas

  • Immunology

Cite this

Simpson, E., Robinson, P. J., Chandler, P., Millrain, M. M., Pircher, H. P., Brandle, D., ... Mellor, A. (1994). Separation of thymic education from antigen presenting functions of major histocompatibility complex class I molecules. Immunology, 81(1), 132-136.

Separation of thymic education from antigen presenting functions of major histocompatibility complex class I molecules. / Simpson, E.; Robinson, P. J.; Chandler, P.; Millrain, M. M.; Pircher, H. P.; Brandle, D.; Tomlinson, P.; Antoniou, J.; Mellor, A.

In: Immunology, Vol. 81, No. 1, 01.01.1994, p. 132-136.

Research output: Contribution to journalArticle

Simpson, E, Robinson, PJ, Chandler, P, Millrain, MM, Pircher, HP, Brandle, D, Tomlinson, P, Antoniou, J & Mellor, A 1994, 'Separation of thymic education from antigen presenting functions of major histocompatibility complex class I molecules', Immunology, vol. 81, no. 1, pp. 132-136.
Simpson E, Robinson PJ, Chandler P, Millrain MM, Pircher HP, Brandle D et al. Separation of thymic education from antigen presenting functions of major histocompatibility complex class I molecules. Immunology. 1994 Jan 1;81(1):132-136.
Simpson, E. ; Robinson, P. J. ; Chandler, P. ; Millrain, M. M. ; Pircher, H. P. ; Brandle, D. ; Tomlinson, P. ; Antoniou, J. ; Mellor, A. / Separation of thymic education from antigen presenting functions of major histocompatibility complex class I molecules. In: Immunology. 1994 ; Vol. 81, No. 1. pp. 132-136.
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