Separation of thymic education from antigen presenting functions of major histocompatibility complex class I molecules

E. Simpson, P. J. Robinson, P. Chandler, M. M. Millrain, H. P. Pircher, D. Brandle, P. Tomlinson, J. Antoniou, A. Mellor

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

Participation of transmembrane (TM) and glycosyl-phosphatidylinositol (GPI) anchored H-2Db molecules in antigen presentation and thymic selection events was investigated using transgenic mice. Both GPI-Db and TM-Db can efficiently present H-Y antigen, influenza and lymphocytic choriomeningitis virus (LCMV) peptides to primed cytotoxic. H-2Db-restricted T cells. Transgenic mice expressing GPI-Db, although unable to reject TM-Db skin grafts, nevertheless generate secondary CTL responses which can lyse TM-Db- bearing targets, indicating that GPI-Db mice fail to delete all TM-Db- reactive T cells. Furthermore, double-transgenic mice bearing GPI-Db and a T-cell receptor (TcR) for H-2Db + LCMV do not positively select receptor positive, CD8+CD4- T cells. This paradoxical behaviour of GPI-Db molecules suggests that the structural requirements for antigen presentation and thymic selection by class I molecules are different and may explain why GPI-linked class I molecules, such as Qa-2, do not appear to function as restriction elements in vivo.

Original languageEnglish (US)
Pages (from-to)132-136
Number of pages5
JournalImmunology
Volume81
Issue number1
StatePublished - Jan 1 1994

ASJC Scopus subject areas

  • Immunology

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    Simpson, E., Robinson, P. J., Chandler, P., Millrain, M. M., Pircher, H. P., Brandle, D., Tomlinson, P., Antoniou, J., & Mellor, A. (1994). Separation of thymic education from antigen presenting functions of major histocompatibility complex class I molecules. Immunology, 81(1), 132-136.