Seprafilm (modified hyaluronic acid and carboxymethylcellulose) acts as a physical barrier

L. April Gago, Ghassan M. Saed, Subodhsingh Chauhan, Eslam F. Elhammady, Michael P. Diamond

Research output: Contribution to journalArticle

62 Scopus citations

Abstract

Objective: To characterize the molecular changes that occur in normal fibroblasts, adhesion fibroblasts, and mesothelial cells as a result of exposure to modified hyaluronic acid and carboxymethylcellulose (Seprafilm). Setting: University research laboratory. Design: Human mesothelial and fibroblast cell culture. Main Outcome Measure(s): Multiplex reverse transcriptase polymerase chain reaction was used to examine control and Seprafilm-treated normal peritoneal fibroblasts, adhesion fibroblasts, and mesothelial cells in culture for levels of messenger RNA from molecules known to be associated with adhesion development (transforming growth factor-β1, type I collagen, matrix metalloproteinase-1, matrix metalloproteinase-2, tissue inhibitor of metalloproteinase-1, and tissue plasminogen activator). Result(s): Seprafilm treatment of normal peritoneal fibroblasts, adhesion fibroblasts and mesothelial cells did not alter the expression of markers examined. Conclusion(s): In the absence of a biological effect of Seprafilm on markers known to be involved in postoperative adhesion development, the ability of Seprafilm to reduce postoperative adhesions is most likely caused by its effect as a physical barrier.

Original languageEnglish (US)
Pages (from-to)612-616
Number of pages5
JournalFertility and sterility
Volume80
Issue number3
DOIs
StatePublished - Sep 1 2003
Externally publishedYes

Keywords

  • Adhesion barriers
  • Adhesion fibroblasts
  • Adhesions
  • Matrix metalloproteinase-1
  • Matrix metalloproteinase-2
  • Seprafilm
  • Tissue inhibitor of metalloproteinase-1
  • Tissue plasminogen activator
  • Transforming growth factor-β1
  • Type I collagen

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynecology

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