Sequence of the mouse Q4 class I gene and characterization of the gene product

Peter J. Robinson, Dorian Bevec, Andrew L. Mellor, Elisabeth H. Weiss

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

The Q4 class I gene has been shown to participate in gene conversion events within the mouse major histocompatibility complex. Its complete genomic nucleotide sequence has been determined. The 5′ half of Q4 resembles H-2 genes more strongly than other Q genes. Its 3′ end, in contrast, is Q-like and contains a translational stop signal in exon 5 which predicts a polypeptide with an incomplete membrane spanning segment. The presence of two inverted B1 repeats suggests that part of the Q4 gene may be mobile within the genome. Gene transfer experiments have shown that the Q4 gene encodes a β2-microglobulin associated polypeptide of Mr 41 000. A similar protein was found in activated mouse spleen cells. The Q4 polypeptide was found to be secreted both by spleen cells and by transfected fibroblasts and was not detectable on the cell surface. Antibody binding and two-dimensional gel electrophoresis indicate that the Q4 molecule is identical to a mouse class I polypeptide, Qb-1, which has been previously described.

Original languageEnglish (US)
Pages (from-to)79-86
Number of pages8
JournalImmunogenetics
Volume27
Issue number2
DOIs
StatePublished - Feb 1 1988

Fingerprint

MHC Class I Genes
Peptides
Genes
Spleen
vif Genes
Gene Conversion
Electrophoresis, Gel, Two-Dimensional
Major Histocompatibility Complex
Exons
Fibroblasts
Genome
Membranes
Antibodies
Proteins

ASJC Scopus subject areas

  • Immunology
  • Genetics

Cite this

Robinson, P. J., Bevec, D., Mellor, A. L., & Weiss, E. H. (1988). Sequence of the mouse Q4 class I gene and characterization of the gene product. Immunogenetics, 27(2), 79-86. https://doi.org/10.1007/BF00351079

Sequence of the mouse Q4 class I gene and characterization of the gene product. / Robinson, Peter J.; Bevec, Dorian; Mellor, Andrew L.; Weiss, Elisabeth H.

In: Immunogenetics, Vol. 27, No. 2, 01.02.1988, p. 79-86.

Research output: Contribution to journalArticle

Robinson, PJ, Bevec, D, Mellor, AL & Weiss, EH 1988, 'Sequence of the mouse Q4 class I gene and characterization of the gene product', Immunogenetics, vol. 27, no. 2, pp. 79-86. https://doi.org/10.1007/BF00351079
Robinson, Peter J. ; Bevec, Dorian ; Mellor, Andrew L. ; Weiss, Elisabeth H. / Sequence of the mouse Q4 class I gene and characterization of the gene product. In: Immunogenetics. 1988 ; Vol. 27, No. 2. pp. 79-86.
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