Serologic Diagnosis of Lyme Disease using Recombinant Outer Surface Proteins A and B and Flagellin

Erol Fikrig, Edward D. Huguenel, Robert Berland, Daniel Wallace Rahn, John A. Hardin, Richard A. Flavell

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Recombinant (r) outer surface proteins (Osp) A and B, flagellin, and an immunogenic region of flagellin (41-G) from Borrelia burgdorferi were evaluated using immunoblot and ELISA for usefulness as substrates in diagnostic testing for Lyme disease. Antibodies to rOspA, rOspB, and r-flagellin were detected by immunoblot and ELISA using the recombinant proteins. Patients with late disease responded to rOspA, rOspB, and r-flagellin, or only to r-flagellin, whereas patients with early disease showed no response or responded only to r-flagellin. Patients who developed antibodies to r-flagellin also responded to 41-G. The data suggest that recombinant B. burgdorferi antigens can serve as substrates for immunoblot and ELISA, which may be helpful in diagnosing Lyme disease.

Original languageEnglish (US)
Pages (from-to)1127-1132
Number of pages6
JournalJournal of Infectious Diseases
Volume165
Issue number6
DOIs
StatePublished - Jan 1 1992
Externally publishedYes

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Flagellin
Lyme Disease
Borrelia burgdorferi
Enzyme-Linked Immunosorbent Assay
Antibodies
OspA protein
IgA receptor
Recombinant Proteins
Antigens

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

Cite this

Serologic Diagnosis of Lyme Disease using Recombinant Outer Surface Proteins A and B and Flagellin. / Fikrig, Erol; Huguenel, Edward D.; Berland, Robert; Rahn, Daniel Wallace; Hardin, John A.; Flavell, Richard A.

In: Journal of Infectious Diseases, Vol. 165, No. 6, 01.01.1992, p. 1127-1132.

Research output: Contribution to journalArticle

Fikrig, Erol ; Huguenel, Edward D. ; Berland, Robert ; Rahn, Daniel Wallace ; Hardin, John A. ; Flavell, Richard A. / Serologic Diagnosis of Lyme Disease using Recombinant Outer Surface Proteins A and B and Flagellin. In: Journal of Infectious Diseases. 1992 ; Vol. 165, No. 6. pp. 1127-1132.
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