Serologic Diagnosis of Lyme Disease using Recombinant Outer Surface Proteins A and B and Flagellin

Erol Fikrig; Edward D. Huguenel; Robert Berland; Daniel W. Rahn; John A. Hardin; Richard A. Flavell

doi:10.1093/infdis/165.6.1127

Serologic Diagnosis of Lyme Disease using Recombinant Outer Surface Proteins A and B and Flagellin

Erol Fikrig, Edward D. Huguenel, Robert Berland, Daniel W. Rahn, John A. Hardin, Richard A. Flavell

Health Econ Modeling

Research output: Contribution to journal › Article › peer-review

41 Scopus citations

Abstract

Recombinant (r) outer surface proteins (Osp) A and B, flagellin, and an immunogenic region of flagellin (41-G) from Borrelia burgdorferi were evaluated using immunoblot and ELISA for usefulness as substrates in diagnostic testing for Lyme disease. Antibodies to rOspA, rOspB, and r-flagellin were detected by immunoblot and ELISA using the recombinant proteins. Patients with late disease responded to rOspA, rOspB, and r-flagellin, or only to r-flagellin, whereas patients with early disease showed no response or responded only to r-flagellin. Patients who developed antibodies to r-flagellin also responded to 41-G. The data suggest that recombinant B. burgdorferi antigens can serve as substrates for immunoblot and ELISA, which may be helpful in diagnosing Lyme disease.

Original language	English (US)
Pages (from-to)	1127-1132
Number of pages	6
Journal	Journal of Infectious Diseases
Volume	165
Issue number	6
DOIs	https://doi.org/10.1093/infdis/165.6.1127
State	Published - Jun 1992

ASJC Scopus subject areas

General Medicine

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1093/infdis/165.6.1127

Cite this

@article{f5de49d791fc486e88136734abffe89d,

title = "Serologic Diagnosis of Lyme Disease using Recombinant Outer Surface Proteins A and B and Flagellin",

abstract = "Recombinant (r) outer surface proteins (Osp) A and B, flagellin, and an immunogenic region of flagellin (41-G) from Borrelia burgdorferi were evaluated using immunoblot and ELISA for usefulness as substrates in diagnostic testing for Lyme disease. Antibodies to rOspA, rOspB, and r-flagellin were detected by immunoblot and ELISA using the recombinant proteins. Patients with late disease responded to rOspA, rOspB, and r-flagellin, or only to r-flagellin, whereas patients with early disease showed no response or responded only to r-flagellin. Patients who developed antibodies to r-flagellin also responded to 41-G. The data suggest that recombinant B. burgdorferi antigens can serve as substrates for immunoblot and ELISA, which may be helpful in diagnosing Lyme disease.",

author = "Erol Fikrig and Huguenel, {Edward D.} and Robert Berland and Rahn, {Daniel W.} and Hardin, {John A.} and Flavell, {Richard A.}",

year = "1992",

month = jun,

doi = "10.1093/infdis/165.6.1127",

language = "English (US)",

volume = "165",

pages = "1127--1132",

journal = "Journal of Infectious Diseases",

issn = "0022-1899",

publisher = "Oxford University Press",

number = "6",

}

TY - JOUR

T1 - Serologic Diagnosis of Lyme Disease using Recombinant Outer Surface Proteins A and B and Flagellin

AU - Fikrig, Erol

AU - Huguenel, Edward D.

AU - Berland, Robert

AU - Rahn, Daniel W.

AU - Hardin, John A.

AU - Flavell, Richard A.

PY - 1992/6

Y1 - 1992/6

N2 - Recombinant (r) outer surface proteins (Osp) A and B, flagellin, and an immunogenic region of flagellin (41-G) from Borrelia burgdorferi were evaluated using immunoblot and ELISA for usefulness as substrates in diagnostic testing for Lyme disease. Antibodies to rOspA, rOspB, and r-flagellin were detected by immunoblot and ELISA using the recombinant proteins. Patients with late disease responded to rOspA, rOspB, and r-flagellin, or only to r-flagellin, whereas patients with early disease showed no response or responded only to r-flagellin. Patients who developed antibodies to r-flagellin also responded to 41-G. The data suggest that recombinant B. burgdorferi antigens can serve as substrates for immunoblot and ELISA, which may be helpful in diagnosing Lyme disease.

AB - Recombinant (r) outer surface proteins (Osp) A and B, flagellin, and an immunogenic region of flagellin (41-G) from Borrelia burgdorferi were evaluated using immunoblot and ELISA for usefulness as substrates in diagnostic testing for Lyme disease. Antibodies to rOspA, rOspB, and r-flagellin were detected by immunoblot and ELISA using the recombinant proteins. Patients with late disease responded to rOspA, rOspB, and r-flagellin, or only to r-flagellin, whereas patients with early disease showed no response or responded only to r-flagellin. Patients who developed antibodies to r-flagellin also responded to 41-G. The data suggest that recombinant B. burgdorferi antigens can serve as substrates for immunoblot and ELISA, which may be helpful in diagnosing Lyme disease.

UR - http://www.scopus.com/inward/record.url?scp=0026581407&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026581407&partnerID=8YFLogxK

U2 - 10.1093/infdis/165.6.1127

DO - 10.1093/infdis/165.6.1127

M3 - Article

C2 - 1583333

AN - SCOPUS:0026581407

SN - 0022-1899

VL - 165

SP - 1127

EP - 1132

JO - Journal of Infectious Diseases

JF - Journal of Infectious Diseases

IS - 6

ER -

Serologic Diagnosis of Lyme Disease using Recombinant Outer Surface Proteins A and B and Flagellin

Abstract

ASJC Scopus subject areas

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this