TY - JOUR
T1 - Serotonin-induced coronary spasm in a swine model
T2 - A minor role of defective endothelium-derived relaxing factor
AU - Fukai, Tohru
AU - Egashira, Kensuke
AU - Hata, Hiroshi
AU - Numaguchi, Koutaro
AU - Ohara, Yuichi
AU - Takahashi, Teisuke
AU - Tomoike, Hitonobu
AU - Takeshita, Akira
PY - 1993/10
Y1 - 1993/10
N2 - Background. Coronary spasm may be caused by endothelial dysfunction, vascular smooth muscle hyperreactivity, or both. We aimed to determine the relative role of endothelial dysfunction and vascular smooth muscle hyperreactivity in the pathogenesis of coronary artery spasm in the swine model in vivo. Methods and Results. In Göttingen miniature pigs given a high cholesterol diet, a segment of the left coronary artery was denuded and irradiated with x-ray (total, 30 Gy). Three months after endothelial denudation and irradiation, vasomotor responses of the denuded and control sites to agonists were assessed by quantitative arteriography. Serotonin (10 μg/kg) provoked coronary spasm at the denuded site (diameter reduction, 79±6%) associated with ST elevation but not at the nondenuded control site (21±6%). Intracoronary infusion of Nω-nitro-L-arginine methyl ester (LNNA, an inhibitor of endothelium-derived nitric oxide) of 1 and 3 mg/kg potentiated constriction evoked with serotonin (1, 3, 10 μg/kg) at the control site but did not alter it at the denuded site. However, serotonin-induced constriction after LNNA was still less at the control site (31±3%) than at the denuded site (80±5%). Endothelium-dependent vasodilation with substance P (0.1, 1, 10 ng/kg), which was inhibited by LNNA, was less (P<.01) at the denuded site than at the control site, whereas vasodilation with the nitrovasodilator SIN-1 (0.1, 1, 10 ng/kg) was comparable between the two sites. Histological study revealed regenerated endothelial cells and intimai thickening at the denuded site. Conclusions. The results suggest that the denuded segment of the coronary artery with regenerated endothelium was associated with defective endothelium-dependent vasodilation mediated by nitric oxide and vascular smooth muscle hyperreactivity to serotonin. However, provocation of coronary spasm with serotonin resulted primarily from vascular smooth muscle hyperreactivity but not by defective nitric oxide production in this swine model.
AB - Background. Coronary spasm may be caused by endothelial dysfunction, vascular smooth muscle hyperreactivity, or both. We aimed to determine the relative role of endothelial dysfunction and vascular smooth muscle hyperreactivity in the pathogenesis of coronary artery spasm in the swine model in vivo. Methods and Results. In Göttingen miniature pigs given a high cholesterol diet, a segment of the left coronary artery was denuded and irradiated with x-ray (total, 30 Gy). Three months after endothelial denudation and irradiation, vasomotor responses of the denuded and control sites to agonists were assessed by quantitative arteriography. Serotonin (10 μg/kg) provoked coronary spasm at the denuded site (diameter reduction, 79±6%) associated with ST elevation but not at the nondenuded control site (21±6%). Intracoronary infusion of Nω-nitro-L-arginine methyl ester (LNNA, an inhibitor of endothelium-derived nitric oxide) of 1 and 3 mg/kg potentiated constriction evoked with serotonin (1, 3, 10 μg/kg) at the control site but did not alter it at the denuded site. However, serotonin-induced constriction after LNNA was still less at the control site (31±3%) than at the denuded site (80±5%). Endothelium-dependent vasodilation with substance P (0.1, 1, 10 ng/kg), which was inhibited by LNNA, was less (P<.01) at the denuded site than at the control site, whereas vasodilation with the nitrovasodilator SIN-1 (0.1, 1, 10 ng/kg) was comparable between the two sites. Histological study revealed regenerated endothelial cells and intimai thickening at the denuded site. Conclusions. The results suggest that the denuded segment of the coronary artery with regenerated endothelium was associated with defective endothelium-dependent vasodilation mediated by nitric oxide and vascular smooth muscle hyperreactivity to serotonin. However, provocation of coronary spasm with serotonin resulted primarily from vascular smooth muscle hyperreactivity but not by defective nitric oxide production in this swine model.
KW - Atherosclerosis
KW - Endothelium
KW - Nitric oxide
KW - Substance P
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M3 - Article
C2 - 7691432
AN - SCOPUS:0027449418
SN - 0009-7322
VL - 88
SP - 1922
EP - 1930
JO - Circulation
JF - Circulation
IS - 4
ER -