Serum inflammatory protein profiles in patients with chronic rhinosinusitis undergoing sinus surgery: A preliminary analysis

Adam M. Becker, Subinoy Das, Zhongsheng Xia, Zhongmin Liu, Bao Ling Adam, Stilianos E. Kountakis

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Background: The specific aim of this study was to evaluate surface-enhanced laser desorption/ionization time-of-flight mass spectroscopy (SELDI-TOF-MS) as a clinical screening tool in differentiating patients with chronic rhinosinusitis (CRS) from healthy control patients. Methods: Serum samples were prospectively collected at a tertiary care academic medical center from 96 CRS patients who underwent functional endoscopic sinus surgery (FESS) and 38 control volunteers who were negatively screened according to the Rhinosinusitis Task Force guidelines on CRS. SELDI-TOF-MS was performed on serum samples to detect protein profiles in the range of 1-100 kDa. Analysis of spectral data was performed to determine the sensitivity and specificity of SELDI-TOF-MS in distinguishing patients with CRS requiring FESS from healthy controls and to determine potential markers for protein purification. Results: Serum protein profiles generated from SELDI-TOF-MS in the range of 1-100 kDa were analyzed. Classification and regression tree analysis based on peak expression correctly classified patients with CRS with 77.1% sensitivity and 65.8% specificity, a positive predictive value of 88%, and a negative predictive value of 53%. Underexpression of a protein peak at 8.4 kDa was associated with CRS in 77% of cases. Conclusion: SELDI-TOF-MS serum protein profile analysis is able to distinguish CRS patients requiring FESS from healthy, negatively screened controls with a sensitivity of 77.1% and specificity of 65.8%. Additional investigation is required to determine if SELDI-TOF-MS will make an effective clinical tool.

Original languageEnglish (US)
Pages (from-to)139-143
Number of pages5
JournalAmerican Journal of Rhinology
Volume22
Issue number2
DOIs
StatePublished - Mar 1 2008

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Blood Proteins
Mass Spectrometry
Lasers
Sensitivity and Specificity
Proteins
Advisory Committees
Tertiary Healthcare
Serum
Volunteers
Regression Analysis
Guidelines

Keywords

  • Chronic sinusitis
  • Proteomics
  • Rhinosinusitis
  • SELDI
  • Serum
  • Sinus surgery
  • Sinusitis

ASJC Scopus subject areas

  • Otorhinolaryngology

Cite this

Serum inflammatory protein profiles in patients with chronic rhinosinusitis undergoing sinus surgery : A preliminary analysis. / Becker, Adam M.; Das, Subinoy; Xia, Zhongsheng; Liu, Zhongmin; Adam, Bao Ling; Kountakis, Stilianos E.

In: American Journal of Rhinology, Vol. 22, No. 2, 01.03.2008, p. 139-143.

Research output: Contribution to journalArticle

Becker, Adam M. ; Das, Subinoy ; Xia, Zhongsheng ; Liu, Zhongmin ; Adam, Bao Ling ; Kountakis, Stilianos E. / Serum inflammatory protein profiles in patients with chronic rhinosinusitis undergoing sinus surgery : A preliminary analysis. In: American Journal of Rhinology. 2008 ; Vol. 22, No. 2. pp. 139-143.
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abstract = "Background: The specific aim of this study was to evaluate surface-enhanced laser desorption/ionization time-of-flight mass spectroscopy (SELDI-TOF-MS) as a clinical screening tool in differentiating patients with chronic rhinosinusitis (CRS) from healthy control patients. Methods: Serum samples were prospectively collected at a tertiary care academic medical center from 96 CRS patients who underwent functional endoscopic sinus surgery (FESS) and 38 control volunteers who were negatively screened according to the Rhinosinusitis Task Force guidelines on CRS. SELDI-TOF-MS was performed on serum samples to detect protein profiles in the range of 1-100 kDa. Analysis of spectral data was performed to determine the sensitivity and specificity of SELDI-TOF-MS in distinguishing patients with CRS requiring FESS from healthy controls and to determine potential markers for protein purification. Results: Serum protein profiles generated from SELDI-TOF-MS in the range of 1-100 kDa were analyzed. Classification and regression tree analysis based on peak expression correctly classified patients with CRS with 77.1{\%} sensitivity and 65.8{\%} specificity, a positive predictive value of 88{\%}, and a negative predictive value of 53{\%}. Underexpression of a protein peak at 8.4 kDa was associated with CRS in 77{\%} of cases. Conclusion: SELDI-TOF-MS serum protein profile analysis is able to distinguish CRS patients requiring FESS from healthy, negatively screened controls with a sensitivity of 77.1{\%} and specificity of 65.8{\%}. Additional investigation is required to determine if SELDI-TOF-MS will make an effective clinical tool.",
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