Serum levels of microRNAs can specifically predict liver injury of chronic hepatitis B

Hui Zhang, Qing Ya Li, Zhi Zhong Guo, Yan Guan, Jia Du, Yi Yu Lu, Yi Yang Hu, Ping Liu, Shuang Huang, Shi Bing Su

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Aim: To investigate whether circulating microRNAs (miRNAs) can serve as molecular markers to predict liver injury resulted from chronic hepatitis B (CHB). Methods: The profiles of serum miRNA expression were first generated with serum samples collected from 10 patients with CHB and 10 healthy donors (Ctrls) by microarray analysis. The levels of several miRNAs were further quantitated by real-time reverse transcription polymerase chain reaction with serum samples from another 24 CHB patients and 24 Ctrls. Serum samples of 20 patients with nonalcohlic steatohepatitis (NASH) were also included for comparison. The comparison in the levels of miRNAs between groups (CHB, NASH and Ctrl) was analyzed with Mann-Whitney U -test. The correlation between miRNAs and clinical pathoparameters was analyzed using Spearman correlation analysis or canonical correlation analysis. The receiver-operator characteristic (ROC) curves were also generated to determine the specificity and sensitivity of each individual miRNA in distinguishing patients with CHB from Ctrls. Results: miRNA profile analysis showed that 34 miRNAs were differentially expressed between CHB and Ctrl subjects, in which 12 were up-regulated and 22 down-regulated in CHB subject (fold change > 2.0 and P < 0.01). The median levels of miR-122, -572, -575 and -638 were significantly higher (P < 1.00 × 10-5) while miR-744 significantly lower (P < 1.00 × 10-6) in CHB compared with the Ctrl. The levels of miR-122, -572 and -638 were also higher (P < 1.00 × 10-3) while the level of miR-744 lower in CHB (P < 0.05) than in NASH, although the difference between them was not as significant as that between CHB and Ctrl. ROC curve analysis revealed that the levels of miR-122, -572, -575, -638 and -744 in serum were sensitive and specific enough to distinguish CHB, NASH and Ctrl. Multivariate analysis further showed that the levels of these miRNAs were correlated with the liver function parameters. Most significantly, it was the scatter plot of principal component with the levels of these miRNAs, but not the parameters of liver function, which clearly distinguished CHB, NASH and Ctrl subjects. Conclusion: Serum levels of miR-122, -572, -575, -638 and -744 are deregulated in patients with CHB or NASH. The levels of these miRNAs may serve as potential biomarkers for liver injury caused by CHB and NASH.

Original languageEnglish (US)
Pages (from-to)5188-5196
Number of pages9
JournalWorld Journal of Gastroenterology
Volume18
Issue number37
DOIs
StatePublished - Oct 29 2012

Fingerprint

Chronic Hepatitis B
MicroRNAs
Liver
Wounds and Injuries
Fatty Liver
Serum
Microarray Analysis
Nonparametric Statistics
Reverse Transcription
Multivariate Analysis

Keywords

  • Chronic hepatitis B
  • Liver injury
  • Nonalcohlic steatohepatitis
  • Serum microRNAs

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Zhang, H., Li, Q. Y., Guo, Z. Z., Guan, Y., Du, J., Lu, Y. Y., ... Su, S. B. (2012). Serum levels of microRNAs can specifically predict liver injury of chronic hepatitis B. World Journal of Gastroenterology, 18(37), 5188-5196. https://doi.org/10.3748/wjg.v18.i37.5188

Serum levels of microRNAs can specifically predict liver injury of chronic hepatitis B. / Zhang, Hui; Li, Qing Ya; Guo, Zhi Zhong; Guan, Yan; Du, Jia; Lu, Yi Yu; Hu, Yi Yang; Liu, Ping; Huang, Shuang; Su, Shi Bing.

In: World Journal of Gastroenterology, Vol. 18, No. 37, 29.10.2012, p. 5188-5196.

Research output: Contribution to journalArticle

Zhang, H, Li, QY, Guo, ZZ, Guan, Y, Du, J, Lu, YY, Hu, YY, Liu, P, Huang, S & Su, SB 2012, 'Serum levels of microRNAs can specifically predict liver injury of chronic hepatitis B', World Journal of Gastroenterology, vol. 18, no. 37, pp. 5188-5196. https://doi.org/10.3748/wjg.v18.i37.5188
Zhang, Hui ; Li, Qing Ya ; Guo, Zhi Zhong ; Guan, Yan ; Du, Jia ; Lu, Yi Yu ; Hu, Yi Yang ; Liu, Ping ; Huang, Shuang ; Su, Shi Bing. / Serum levels of microRNAs can specifically predict liver injury of chronic hepatitis B. In: World Journal of Gastroenterology. 2012 ; Vol. 18, No. 37. pp. 5188-5196.
@article{df80848775ab471d8f3e2f89fb115935,
title = "Serum levels of microRNAs can specifically predict liver injury of chronic hepatitis B",
abstract = "Aim: To investigate whether circulating microRNAs (miRNAs) can serve as molecular markers to predict liver injury resulted from chronic hepatitis B (CHB). Methods: The profiles of serum miRNA expression were first generated with serum samples collected from 10 patients with CHB and 10 healthy donors (Ctrls) by microarray analysis. The levels of several miRNAs were further quantitated by real-time reverse transcription polymerase chain reaction with serum samples from another 24 CHB patients and 24 Ctrls. Serum samples of 20 patients with nonalcohlic steatohepatitis (NASH) were also included for comparison. The comparison in the levels of miRNAs between groups (CHB, NASH and Ctrl) was analyzed with Mann-Whitney U -test. The correlation between miRNAs and clinical pathoparameters was analyzed using Spearman correlation analysis or canonical correlation analysis. The receiver-operator characteristic (ROC) curves were also generated to determine the specificity and sensitivity of each individual miRNA in distinguishing patients with CHB from Ctrls. Results: miRNA profile analysis showed that 34 miRNAs were differentially expressed between CHB and Ctrl subjects, in which 12 were up-regulated and 22 down-regulated in CHB subject (fold change > 2.0 and P < 0.01). The median levels of miR-122, -572, -575 and -638 were significantly higher (P < 1.00 × 10-5) while miR-744 significantly lower (P < 1.00 × 10-6) in CHB compared with the Ctrl. The levels of miR-122, -572 and -638 were also higher (P < 1.00 × 10-3) while the level of miR-744 lower in CHB (P < 0.05) than in NASH, although the difference between them was not as significant as that between CHB and Ctrl. ROC curve analysis revealed that the levels of miR-122, -572, -575, -638 and -744 in serum were sensitive and specific enough to distinguish CHB, NASH and Ctrl. Multivariate analysis further showed that the levels of these miRNAs were correlated with the liver function parameters. Most significantly, it was the scatter plot of principal component with the levels of these miRNAs, but not the parameters of liver function, which clearly distinguished CHB, NASH and Ctrl subjects. Conclusion: Serum levels of miR-122, -572, -575, -638 and -744 are deregulated in patients with CHB or NASH. The levels of these miRNAs may serve as potential biomarkers for liver injury caused by CHB and NASH.",
keywords = "Chronic hepatitis B, Liver injury, Nonalcohlic steatohepatitis, Serum microRNAs",
author = "Hui Zhang and Li, {Qing Ya} and Guo, {Zhi Zhong} and Yan Guan and Jia Du and Lu, {Yi Yu} and Hu, {Yi Yang} and Ping Liu and Shuang Huang and Su, {Shi Bing}",
year = "2012",
month = "10",
day = "29",
doi = "10.3748/wjg.v18.i37.5188",
language = "English (US)",
volume = "18",
pages = "5188--5196",
journal = "World Journal of Gastroenterology",
issn = "1007-9327",
publisher = "WJG Press",
number = "37",

}

TY - JOUR

T1 - Serum levels of microRNAs can specifically predict liver injury of chronic hepatitis B

AU - Zhang, Hui

AU - Li, Qing Ya

AU - Guo, Zhi Zhong

AU - Guan, Yan

AU - Du, Jia

AU - Lu, Yi Yu

AU - Hu, Yi Yang

AU - Liu, Ping

AU - Huang, Shuang

AU - Su, Shi Bing

PY - 2012/10/29

Y1 - 2012/10/29

N2 - Aim: To investigate whether circulating microRNAs (miRNAs) can serve as molecular markers to predict liver injury resulted from chronic hepatitis B (CHB). Methods: The profiles of serum miRNA expression were first generated with serum samples collected from 10 patients with CHB and 10 healthy donors (Ctrls) by microarray analysis. The levels of several miRNAs were further quantitated by real-time reverse transcription polymerase chain reaction with serum samples from another 24 CHB patients and 24 Ctrls. Serum samples of 20 patients with nonalcohlic steatohepatitis (NASH) were also included for comparison. The comparison in the levels of miRNAs between groups (CHB, NASH and Ctrl) was analyzed with Mann-Whitney U -test. The correlation between miRNAs and clinical pathoparameters was analyzed using Spearman correlation analysis or canonical correlation analysis. The receiver-operator characteristic (ROC) curves were also generated to determine the specificity and sensitivity of each individual miRNA in distinguishing patients with CHB from Ctrls. Results: miRNA profile analysis showed that 34 miRNAs were differentially expressed between CHB and Ctrl subjects, in which 12 were up-regulated and 22 down-regulated in CHB subject (fold change > 2.0 and P < 0.01). The median levels of miR-122, -572, -575 and -638 were significantly higher (P < 1.00 × 10-5) while miR-744 significantly lower (P < 1.00 × 10-6) in CHB compared with the Ctrl. The levels of miR-122, -572 and -638 were also higher (P < 1.00 × 10-3) while the level of miR-744 lower in CHB (P < 0.05) than in NASH, although the difference between them was not as significant as that between CHB and Ctrl. ROC curve analysis revealed that the levels of miR-122, -572, -575, -638 and -744 in serum were sensitive and specific enough to distinguish CHB, NASH and Ctrl. Multivariate analysis further showed that the levels of these miRNAs were correlated with the liver function parameters. Most significantly, it was the scatter plot of principal component with the levels of these miRNAs, but not the parameters of liver function, which clearly distinguished CHB, NASH and Ctrl subjects. Conclusion: Serum levels of miR-122, -572, -575, -638 and -744 are deregulated in patients with CHB or NASH. The levels of these miRNAs may serve as potential biomarkers for liver injury caused by CHB and NASH.

AB - Aim: To investigate whether circulating microRNAs (miRNAs) can serve as molecular markers to predict liver injury resulted from chronic hepatitis B (CHB). Methods: The profiles of serum miRNA expression were first generated with serum samples collected from 10 patients with CHB and 10 healthy donors (Ctrls) by microarray analysis. The levels of several miRNAs were further quantitated by real-time reverse transcription polymerase chain reaction with serum samples from another 24 CHB patients and 24 Ctrls. Serum samples of 20 patients with nonalcohlic steatohepatitis (NASH) were also included for comparison. The comparison in the levels of miRNAs between groups (CHB, NASH and Ctrl) was analyzed with Mann-Whitney U -test. The correlation between miRNAs and clinical pathoparameters was analyzed using Spearman correlation analysis or canonical correlation analysis. The receiver-operator characteristic (ROC) curves were also generated to determine the specificity and sensitivity of each individual miRNA in distinguishing patients with CHB from Ctrls. Results: miRNA profile analysis showed that 34 miRNAs were differentially expressed between CHB and Ctrl subjects, in which 12 were up-regulated and 22 down-regulated in CHB subject (fold change > 2.0 and P < 0.01). The median levels of miR-122, -572, -575 and -638 were significantly higher (P < 1.00 × 10-5) while miR-744 significantly lower (P < 1.00 × 10-6) in CHB compared with the Ctrl. The levels of miR-122, -572 and -638 were also higher (P < 1.00 × 10-3) while the level of miR-744 lower in CHB (P < 0.05) than in NASH, although the difference between them was not as significant as that between CHB and Ctrl. ROC curve analysis revealed that the levels of miR-122, -572, -575, -638 and -744 in serum were sensitive and specific enough to distinguish CHB, NASH and Ctrl. Multivariate analysis further showed that the levels of these miRNAs were correlated with the liver function parameters. Most significantly, it was the scatter plot of principal component with the levels of these miRNAs, but not the parameters of liver function, which clearly distinguished CHB, NASH and Ctrl subjects. Conclusion: Serum levels of miR-122, -572, -575, -638 and -744 are deregulated in patients with CHB or NASH. The levels of these miRNAs may serve as potential biomarkers for liver injury caused by CHB and NASH.

KW - Chronic hepatitis B

KW - Liver injury

KW - Nonalcohlic steatohepatitis

KW - Serum microRNAs

UR - http://www.scopus.com/inward/record.url?scp=84867835551&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84867835551&partnerID=8YFLogxK

U2 - 10.3748/wjg.v18.i37.5188

DO - 10.3748/wjg.v18.i37.5188

M3 - Article

VL - 18

SP - 5188

EP - 5196

JO - World Journal of Gastroenterology

JF - World Journal of Gastroenterology

SN - 1007-9327

IS - 37

ER -