Sesamin attenuates behavioral, biochemical and histological alterations induced by reversible middle cerebral artery occlusion in the rats

Mohd Moshahid Khan, Tauheed Ishrat, Ajmal Ahmad, MD Nasrul Hoda, Mohammad Badruzzaman Khan, Gulrana Khuwaja, Pallavi Srivastava, Syed Shadab Raza, Fakhrul Islam, Saif Ahmad

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Restoration of blood flow to an ischemic brain region is associated with generation of reactive oxygen species (ROS) with consequent reperfusion injury. ROS cause lipid peroxidation, protein oxidation, and DNA damage, all of which are deleterious to cells. So diminishing the production of free radicals and scavenging them may be a successful therapeutic strategy for the protection of brain tissue in cerebral stroke. The present study investigated the neuroprotective effect of sesamin (Sn) to reduce brain injury after middle cerebral artery occlusion (MCAO). The middle cerebral artery (MCA) of adult male Wistar rat was occluded for 2 h and reperfused for 22 h. Sesamin is the most abundant lignan in sesame seed oil is a potent antioxidant. Sesamin (30 mg/kg) was given orally twice, 30 min before the onset of ischemia and 12 h after reperfusion. The initial investigations revealed that sesamin reduced the neurological deficits in terms of behavior and reduced the level of thiobarbituric acid reactive species (TBARS), and protein carbonyl (PC) in the different areas of the brain when compared with the MCAO group. A significantly depleted level of glutathione and its dependent enzymes (glutathione peroxidase [GPx] and glutathione reductase [GR]) in MCAO group were protected significantly in MCAO group treated with sesamin. The present study suggests that sesamin may be able to attenuate the ischemic cell death and plays a crucial role as a neuroprotectant in regulating levels of reactive oxygen species in the rat brain. Thus, sesamin may be a potential compound in stroke therapy.

Original languageEnglish (US)
Pages (from-to)255-263
Number of pages9
JournalChemico-Biological Interactions
Volume183
Issue number1
DOIs
StatePublished - Jan 5 2010

Fingerprint

Middle Cerebral Artery Infarction
Rats
Brain
Reactive Oxygen Species
Neuroprotective Agents
Stroke
Sesame Oil
Oilseeds
Lignans
Glutathione Reductase
Scavenging
Middle Cerebral Artery
Cell death
Glutathione Peroxidase
sesamin
Reperfusion Injury
Brain Injuries
Lipid Peroxidation
DNA Damage
Restoration

Keywords

  • Middle cerebral artery occlusion
  • Neuroprotection
  • Oxidative stress
  • Sesamin
  • Stroke

ASJC Scopus subject areas

  • Toxicology

Cite this

Sesamin attenuates behavioral, biochemical and histological alterations induced by reversible middle cerebral artery occlusion in the rats. / Khan, Mohd Moshahid; Ishrat, Tauheed; Ahmad, Ajmal; Hoda, MD Nasrul; Khan, Mohammad Badruzzaman; Khuwaja, Gulrana; Srivastava, Pallavi; Raza, Syed Shadab; Islam, Fakhrul; Ahmad, Saif.

In: Chemico-Biological Interactions, Vol. 183, No. 1, 05.01.2010, p. 255-263.

Research output: Contribution to journalArticle

Khan, Mohd Moshahid ; Ishrat, Tauheed ; Ahmad, Ajmal ; Hoda, MD Nasrul ; Khan, Mohammad Badruzzaman ; Khuwaja, Gulrana ; Srivastava, Pallavi ; Raza, Syed Shadab ; Islam, Fakhrul ; Ahmad, Saif. / Sesamin attenuates behavioral, biochemical and histological alterations induced by reversible middle cerebral artery occlusion in the rats. In: Chemico-Biological Interactions. 2010 ; Vol. 183, No. 1. pp. 255-263.
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