Sex differences in leukocyte invasion in injured murine skeletal muscle

Barbara St. Pierre Schneider, Lois A. Correia, Joseph Gerard Cannon

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

The three aims of this study were to describe the time course of leukocyte invasion in injured soleus muscles of male and female mice, to determine if differential subsets of leukocytes accumulate in intramyofiber and interstitial sites, and to determine if significant sex differences exist in invading leukocyte concentrations. Fifty sexually mature C57BL/6J mice (aged 11-12 weeks) underwent unilateral hindlimb muscle injury induced by lengthening contractions. This procedure models the muscle injury that can occur through strenuous exercise or overuse in humans. After 1, 3, 5, or 7 days of recovery, the injured and contralateral, uninjured solei were dissected and prepared for morphologic analysis. We found that leukocytes had invaded injured myofibers at 1-day postinjury for both sexes. Different subsets of leukocytes accumulated within damaged myofibers and the interstitium. Significantly fewer myofibers were invaded by acid phosphatase-positive leukocytes in females. Interstitial ER-BMDM1 leukocyte concentrations peaked in females at 7 days postinjury in comparison to 5 days postinjury in males. These findings expand nursing's knowledge base regarding the potential effect of gender on recovery from acute muscle injury.

Original languageEnglish (US)
Pages (from-to)243-250
Number of pages8
JournalResearch in Nursing and Health
Volume22
Issue number3
DOIs
StatePublished - Jun 1 1999

Fingerprint

Sex Characteristics
Skeletal Muscle
Leukocytes
Muscles
Wounds and Injuries
Knowledge Bases
Hindlimb
Acid Phosphatase
Inbred C57BL Mouse
Nursing

Keywords

  • Inflammation
  • Lengthening contractions
  • Macrophages
  • Muscle injury

ASJC Scopus subject areas

  • Nursing(all)

Cite this

Sex differences in leukocyte invasion in injured murine skeletal muscle. / St. Pierre Schneider, Barbara; Correia, Lois A.; Cannon, Joseph Gerard.

In: Research in Nursing and Health, Vol. 22, No. 3, 01.06.1999, p. 243-250.

Research output: Contribution to journalArticle

St. Pierre Schneider, Barbara ; Correia, Lois A. ; Cannon, Joseph Gerard. / Sex differences in leukocyte invasion in injured murine skeletal muscle. In: Research in Nursing and Health. 1999 ; Vol. 22, No. 3. pp. 243-250.
@article{85f70ef84b514852bd0a95f2ddef15c9,
title = "Sex differences in leukocyte invasion in injured murine skeletal muscle",
abstract = "The three aims of this study were to describe the time course of leukocyte invasion in injured soleus muscles of male and female mice, to determine if differential subsets of leukocytes accumulate in intramyofiber and interstitial sites, and to determine if significant sex differences exist in invading leukocyte concentrations. Fifty sexually mature C57BL/6J mice (aged 11-12 weeks) underwent unilateral hindlimb muscle injury induced by lengthening contractions. This procedure models the muscle injury that can occur through strenuous exercise or overuse in humans. After 1, 3, 5, or 7 days of recovery, the injured and contralateral, uninjured solei were dissected and prepared for morphologic analysis. We found that leukocytes had invaded injured myofibers at 1-day postinjury for both sexes. Different subsets of leukocytes accumulated within damaged myofibers and the interstitium. Significantly fewer myofibers were invaded by acid phosphatase-positive leukocytes in females. Interstitial ER-BMDM1 leukocyte concentrations peaked in females at 7 days postinjury in comparison to 5 days postinjury in males. These findings expand nursing's knowledge base regarding the potential effect of gender on recovery from acute muscle injury.",
keywords = "Inflammation, Lengthening contractions, Macrophages, Muscle injury",
author = "{St. Pierre Schneider}, Barbara and Correia, {Lois A.} and Cannon, {Joseph Gerard}",
year = "1999",
month = "6",
day = "1",
doi = "10.1002/(SICI)1098-240X(199906)22:3<243::AID-NUR6>3.0.CO;2-X",
language = "English (US)",
volume = "22",
pages = "243--250",
journal = "Research in Nursing and Health",
issn = "0160-6891",
publisher = "John Wiley and Sons Inc.",
number = "3",

}

TY - JOUR

T1 - Sex differences in leukocyte invasion in injured murine skeletal muscle

AU - St. Pierre Schneider, Barbara

AU - Correia, Lois A.

AU - Cannon, Joseph Gerard

PY - 1999/6/1

Y1 - 1999/6/1

N2 - The three aims of this study were to describe the time course of leukocyte invasion in injured soleus muscles of male and female mice, to determine if differential subsets of leukocytes accumulate in intramyofiber and interstitial sites, and to determine if significant sex differences exist in invading leukocyte concentrations. Fifty sexually mature C57BL/6J mice (aged 11-12 weeks) underwent unilateral hindlimb muscle injury induced by lengthening contractions. This procedure models the muscle injury that can occur through strenuous exercise or overuse in humans. After 1, 3, 5, or 7 days of recovery, the injured and contralateral, uninjured solei were dissected and prepared for morphologic analysis. We found that leukocytes had invaded injured myofibers at 1-day postinjury for both sexes. Different subsets of leukocytes accumulated within damaged myofibers and the interstitium. Significantly fewer myofibers were invaded by acid phosphatase-positive leukocytes in females. Interstitial ER-BMDM1 leukocyte concentrations peaked in females at 7 days postinjury in comparison to 5 days postinjury in males. These findings expand nursing's knowledge base regarding the potential effect of gender on recovery from acute muscle injury.

AB - The three aims of this study were to describe the time course of leukocyte invasion in injured soleus muscles of male and female mice, to determine if differential subsets of leukocytes accumulate in intramyofiber and interstitial sites, and to determine if significant sex differences exist in invading leukocyte concentrations. Fifty sexually mature C57BL/6J mice (aged 11-12 weeks) underwent unilateral hindlimb muscle injury induced by lengthening contractions. This procedure models the muscle injury that can occur through strenuous exercise or overuse in humans. After 1, 3, 5, or 7 days of recovery, the injured and contralateral, uninjured solei were dissected and prepared for morphologic analysis. We found that leukocytes had invaded injured myofibers at 1-day postinjury for both sexes. Different subsets of leukocytes accumulated within damaged myofibers and the interstitium. Significantly fewer myofibers were invaded by acid phosphatase-positive leukocytes in females. Interstitial ER-BMDM1 leukocyte concentrations peaked in females at 7 days postinjury in comparison to 5 days postinjury in males. These findings expand nursing's knowledge base regarding the potential effect of gender on recovery from acute muscle injury.

KW - Inflammation

KW - Lengthening contractions

KW - Macrophages

KW - Muscle injury

UR - http://www.scopus.com/inward/record.url?scp=0033142638&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033142638&partnerID=8YFLogxK

U2 - 10.1002/(SICI)1098-240X(199906)22:3<243::AID-NUR6>3.0.CO;2-X

DO - 10.1002/(SICI)1098-240X(199906)22:3<243::AID-NUR6>3.0.CO;2-X

M3 - Article

C2 - 10344704

AN - SCOPUS:0033142638

VL - 22

SP - 243

EP - 250

JO - Research in Nursing and Health

JF - Research in Nursing and Health

SN - 0160-6891

IS - 3

ER -