Shear stress-mediated cytoskeletal remodeling and cortactin translocation in pulmonary endothelial cells

Konstantin G. Birukov, Anna A. Birukova, Steven M. Dudek, Alexander D. Verin, Michael T. Crow, Xi Zhan, Natacha DePaola, Joe G.N. Garcia

Research output: Contribution to journalArticle

166 Citations (Scopus)

Abstract

Hemodynamic forces in the form of shear stress (SS) and mechanical strain imposed by circulating blood are recognized factors involved in the control of systemic endothelial cell (EC) cytoskeletal structure and function. However, the effects of acute SS on pulmonary endothelium have not been precisely characterized, nor the mechanism of rapid SS-induced EC cytoskeletal rearrangement understood. We exposed bovine and human pulmonary EC monolayers to laminar SS (10 dynes/ cm2) in a parallel plate flow chamber and observed increased actin stress fiber formation 15 rain after application of flow. Acute SS-induced pronounced cortical cytoskeletal rearrangement characterized by myosin light chain kinase (MLCK)- and Rho-associated kinase (RhoK)-dependent accumulation of diphosphorylated regulatory myosin light chains (MLC) in the cortical actin ring, junctional protein tyrosine phosphorylation, and transient peripheral translocation of cortactin, an actin-binding protein involved in the regulation of actin polymerization. SS-induced cortactin translocation was independent of Erk-1,2 MAP kinase, p60Src, MLCK, or RhoK activities, and unaffected by overexpression of a cortactin mutant lacking four major p60Src phosphorylation sites. However, both SS-induced transient cortactin translocation and cytoskeletal reorientation in response to sustained (24 h) SS was abolished in cells overexpressing either dominant negative Rac 1 or a dominant negative construct of its downstream target, p21-activated kinase (PAK)-1. Our results suggest a potential role for cortactin in the SS-induced EC cortical cytoskeletal remodeling and demonstrate a novel mechanism of Rac GTPase-dependent regulation of the pulmonary endothelial cytoskeleton by SS.

Original languageEnglish (US)
Pages (from-to)453-464
Number of pages12
JournalAmerican journal of respiratory cell and molecular biology
Volume26
Issue number4
DOIs
StatePublished - Jan 1 2002

Fingerprint

Cortactin
Endothelial cells
Shear stress
Endothelial Cells
Lung
Myosin-Light-Chain Kinase
Actins
rho-Associated Kinases
Phosphorylation
p21-Activated Kinases
Microfilament Proteins
Stress Fibers
Myosin Light Chains
Mechanical Stress
Rain
GTP Phosphohydrolases
Mitogen-Activated Protein Kinase 1
Cytoskeleton
Polymerization
Endothelium

ASJC Scopus subject areas

  • Molecular Biology
  • Pulmonary and Respiratory Medicine
  • Clinical Biochemistry
  • Cell Biology

Cite this

Shear stress-mediated cytoskeletal remodeling and cortactin translocation in pulmonary endothelial cells. / Birukov, Konstantin G.; Birukova, Anna A.; Dudek, Steven M.; Verin, Alexander D.; Crow, Michael T.; Zhan, Xi; DePaola, Natacha; Garcia, Joe G.N.

In: American journal of respiratory cell and molecular biology, Vol. 26, No. 4, 01.01.2002, p. 453-464.

Research output: Contribution to journalArticle

Birukov, Konstantin G. ; Birukova, Anna A. ; Dudek, Steven M. ; Verin, Alexander D. ; Crow, Michael T. ; Zhan, Xi ; DePaola, Natacha ; Garcia, Joe G.N. / Shear stress-mediated cytoskeletal remodeling and cortactin translocation in pulmonary endothelial cells. In: American journal of respiratory cell and molecular biology. 2002 ; Vol. 26, No. 4. pp. 453-464.
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