Short-term effect of non-surgical periodontal treatment on local and systemic cytokine levels: Role of hyperglycemia

Mariana de Sousa Rabelo, Giovane Hisse Gomes, Adriana Moura Foz, Amanda Finger Stadler, Christopher W. Cutler, Cristiano Susin, Giuseppe Alexandre Romito

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Background: The effect of non-surgical periodontal treatment on oral and systemic inflammatory mediators in subjects with periodontitis and hyperglycemia remains largely unknown. Therefore, the aim of this clinical study was to compare the short-term effect of non-surgical periodontal treatment on serum, saliva and GCF inflammatory markers levels in GP subjects with or without hyperglycemia. Methods: Sixty subjects divided into four groups of equal size were selected to participate: type 2 diabetics with generalized periodontitis (T2DM + GP), pre-diabetics with GP (PD + GP), normoglycemic subjects with GP (NG + GP), and healthy controls. GCF, serum, and saliva samples were obtained at baseline and 30 days after scaling and root planning (SRP) and the levels of interleukin-1β (IL-1 β), IL-8, IL-6, IL-2, IL-5, IL-4, IL-10, Interferon gamma (IFN-γ), Granulocyte macrophage colony-stimulating factor (GM-CSF) and Tumor necrosis factor-alpha (TNF-α) were determined by ultrasensitive multiplex assay. Clinical periodontal measurements were recorded. Results: SRP yielded significant improvement of all periodontal parameters for all GP groups (p < 0.01). A significant reduction in GCF levels of several cytokines were observed; however, only IL-1B and IFN-γ were consistently reduced post-treatment across all GP groups. Salivary levels of IL-1β were significantly reduced in all GP groups following treatment. No significant differences were observed for serum levels after SRP. Conclusions: Periodontal treatment reduced local inflammatory markers, specifically IL-1B and IFN-γ, irrespective of the diabetes status. Periodontal treatment had no significant effect on serum levels of the inflammatory markers evaluated in this study.

Original languageEnglish (US)
Article number155360
JournalCytokine
Volume138
DOIs
StatePublished - Feb 2021

Keywords

  • Cytokines
  • Gingival crevicular fluid
  • Inflammation
  • Periodontal disease
  • Pre-diabetes
  • Saliva
  • Serum
  • Type 2 diabetes mellitus

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Biochemistry
  • Molecular Biology
  • Hematology

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