Abstract
A highly purified nuclear membrane preparation was obtained from adult rat brain and examined for sialidase activity using GM3, GD1a, GD1b, or N- acetylneuramin lactitol as the substrate. The nuclear membranes contained an appreciable level of sialidase activity; the specific activities toward GM3 and N-acetylneuramin lactitol were 20.5 and 23.8% of the activities in the total brain homogenate, respectively. The sialidase activity in nuclear membranes showed substrate specificity distinct from other membrane-bound sialidases localized in lysosomal membranes, synaptosomal plasma membranes, or myelin membranes. These results strongly suggest the existence of a sialidase activity associated with the nuclear membranes from rat brain.
Original language | English (US) |
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Pages (from-to) | 2205-2208 |
Number of pages | 4 |
Journal | Journal of Neurochemistry |
Volume | 66 |
Issue number | 5 |
DOIs | |
State | Published - May 1996 |
Externally published | Yes |
Keywords
- Brain
- Ganglioside
- Glycoconjugate
- Neuraminidase
- Nuclear membrane
- Sialidase
ASJC Scopus subject areas
- Biochemistry
- Cellular and Molecular Neuroscience