Sialidase activity in nuclear membranes of rat brain

Megumi Saito, Christian L. Fronda, Robert K Yu

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

A highly purified nuclear membrane preparation was obtained from adult rat brain and examined for sialidase activity using GM3, GD1a, GD1b, or N- acetylneuramin lactitol as the substrate. The nuclear membranes contained an appreciable level of sialidase activity; the specific activities toward GM3 and N-acetylneuramin lactitol were 20.5 and 23.8% of the activities in the total brain homogenate, respectively. The sialidase activity in nuclear membranes showed substrate specificity distinct from other membrane-bound sialidases localized in lysosomal membranes, synaptosomal plasma membranes, or myelin membranes. These results strongly suggest the existence of a sialidase activity associated with the nuclear membranes from rat brain.

Original languageEnglish (US)
Pages (from-to)2205-2208
Number of pages4
JournalJournal of Neurochemistry
Volume66
Issue number5
StatePublished - May 1 1996
Externally publishedYes

Fingerprint

Nuclear Envelope
Neuraminidase
Rats
Brain
Membranes
Myelin Sheath
Substrate Specificity
Cell Membrane
Substrates
Cell membranes
neuramin lactitol

Keywords

  • Brain
  • Ganglioside
  • Glycoconjugate
  • Neuraminidase
  • Nuclear membrane
  • Sialidase

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Sialidase activity in nuclear membranes of rat brain. / Saito, Megumi; Fronda, Christian L.; Yu, Robert K.

In: Journal of Neurochemistry, Vol. 66, No. 5, 01.05.1996, p. 2205-2208.

Research output: Contribution to journalArticle

Saito, M, Fronda, CL & Yu, RK 1996, 'Sialidase activity in nuclear membranes of rat brain', Journal of Neurochemistry, vol. 66, no. 5, pp. 2205-2208.
Saito, Megumi ; Fronda, Christian L. ; Yu, Robert K. / Sialidase activity in nuclear membranes of rat brain. In: Journal of Neurochemistry. 1996 ; Vol. 66, No. 5. pp. 2205-2208.
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