Signal transducer and activator of transcription-3 activation is mediated by N-methyl-D-aspartate receptor and L-type voltage-gated Ca2+ channel during cerebral ischemia in rat hippocampus

Hongchun Li, Quanguang Zhang, Guangyi Zhang

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

It has been proved that signal transducer and activator of transcription-3 (STAT3) is expressed and activated following cerebral ischemia/reperfusion in cortex and striatum. Here we investigated the changes in tyrosine phosphorylation and DNA-binding activity of STAT3 in hippocampus in a four-vessel occlusion model of Sprague-Dawley rats. Phospho-STAT3 (in cytoplasm) was enhanced from 5 min and reached its peak level at 10 min of ischemia. While in nucleus, phospho-STAT3 increased from 10 min and then peaked at 30 min of ischemia. Concomitantly, DNA-binding activity of STAT3 demonstrated a similar rule in nucleus extracts. The increased tyrosine phosphorylation and DNA-binding activity of STAT3 in nucleus were blocked by ketamine, an N-methyl-D-aspartate receptor antagonist, or by nifedipine, a L-type voltage-gated Ca2+ channel (L-VGCC) antagonist. These results illustrated that the ionotropic glutamate receptor and L-VGCC are important in mediating STAT3 activation during severe cerebral ischemia.

Original languageEnglish (US)
Pages (from-to)61-64
Number of pages4
JournalNeuroscience Letters
Volume345
Issue number1
DOIs
StatePublished - Jul 10 2003
Externally publishedYes

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Keywords

  • Cerebral ischemia
  • L-Type voltage-gated Ca channel
  • N-Methyl-D-aspartate receptor
  • Rat
  • Signal transducer and activator of transcription-3

ASJC Scopus subject areas

  • Neuroscience(all)

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