Signal transduction through extracellular signal-regulated kinase-like pp57 blocked in differentiated colon carcinoma cells having low levels of c- src kinase

H. Lee, Stephen Hsu, S. Winawer, E. Friedman

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Abstract

Basic fibroblast growth factor (FGF) induced a rapid increase in tyrosine phosphorylation of a 57-kDa cytoplasmic protein with functional myelin basic protein kinase activity and antigenic properties common to mitogen-activated kinases or extracellular signal-regulated kinases. Basic and acidic FGFs and the diacylglycerol diolein used the same signal transduction pathway to activate pp57. These FGFs, like diolein (Lee, H., Ghose-Dastidar, J., Winawer, S., and Friedman, E. (1993) J. Biol. Chem., 268, 5255-5263), increased the cellular concentration of long-chain diacylglycerols within the same short time period as they increased pp57 tyrosine phosphorylation. Both FGF and diolein increased phosphorylation of pp57 on the same V8 protease- generated fragments, suggesting a common pathway for the phosphorylation of pp57. FGF-induced signal transduction through pp57 mitogen-activated kinase led to cell growth in two undifferentiated colon carcinoma cell lines. In contrast, basic FGF neither increased tyrosine phosphorylation of pp57 nor increased cell growth in two colon goblet cell differentiated lines derived from the same parental line as the undifferentiated cells. Both goblet cell lines exhibited levels of protein-tyrosine kinase activity about one-fifth that of the undifferentiated lines. The decrease in tyrosine kinase activity was not due to down-regulation of FGF receptors or their tyrosine kinase activities. c-src kinase-specific activity was decreased 4-5-fold in both goblet cell lines, suggesting a role for c-src in pp57-mediated signal transduction.

Original languageEnglish (US)
Pages (from-to)8181-8187
Number of pages7
JournalJournal of Biological Chemistry
Volume268
Issue number11
StatePublished - Jan 1 1993

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Signal transduction
Phosphorylation
Extracellular Signal-Regulated MAP Kinases
Signal Transduction
Colon
Cells
Goblet Cells
Carcinoma
Protein-Tyrosine Kinases
Tyrosine
Cell Line
Fibroblast Growth Factors
Diglycerides
Cell growth
Fibroblast Growth Factor 2
Mitogens
Phosphotransferases
Fibroblast Growth Factor Receptors
Myelin Basic Protein
Growth

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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Signal transduction through extracellular signal-regulated kinase-like pp57 blocked in differentiated colon carcinoma cells having low levels of c- src kinase. / Lee, H.; Hsu, Stephen; Winawer, S.; Friedman, E.

In: Journal of Biological Chemistry, Vol. 268, No. 11, 01.01.1993, p. 8181-8187.

Research output: Contribution to journalArticle

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