Silencing Breast Cancer Genes Using Morpholino Embedded DNA-Tile-AuNPs Nanostructures

Cansu U. Tunç, Deniz Y. Öztaş, Deniz Uzunoǧlu, Ömer F. Bayrak, Mustafa Çulha

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

There is an ongoing effort to increase the efficiency of gene delivery for the regulation of diseases-related genes. In this report, we demonstrate the efficiency of a DNA-based nanostructure to deliver morpholino antisense oligonucleotides for the upregulated genes in breast cancer as an alternative to the currently used delivery systems. A DNA-tile structure is constructed by embedding antisense oligonucleotides targeting the HER2 and ERα genes. Then, the sticky ends of the DNA-tile nanostructures are hybridized to gold nanoparticles (AuNPs) coated with the complementary oligonucleotides to enhance their cellular uptake. It is found that the morpholino antisense oligonucleotide embedded DNA-tile-AuNPs structure is 30% more effective than the liposome-based system to deliver morpholinos and induce gene silencing in breast cancer cells. The results of the study suggest that the prepared novel nanostructure is an effective and biocompatible carrier that can be used in in vitro gene silencing studies and can be further pursued in in vivo studies.

Original languageEnglish (US)
Pages (from-to)1547-1558
Number of pages12
JournalHuman Gene Therapy
Volume30
Issue number12
DOIs
StatePublished - Dec 2019
Externally publishedYes

Keywords

  • breast cancer
  • DNA nanotechnology
  • DNA-tile
  • gene silencing
  • morpholino

ASJC Scopus subject areas

  • Molecular Medicine
  • Molecular Biology
  • Genetics

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