Simian virus 40 (SV40) T antigen binds specifically to double-stranded DNA but not to single-stranded DNA or DNA/RNA hybrids containing the SV40 regulatory sequences

K. J. Auborn, Rhea-Beth Markowitz, E. Wang, Y. T. Yu, C. Prives

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Simian virus 40 T antigen has been shown previously to bind specifically with high affinity to sites within the regulatory region of double-stranded simian virus 40 DNA. Using competition filter binding and the DNA-binding immunoassay, we show that T antigen did not bind specifically to either early or late single-stranded DNA containing these binding sites. Moreover, T antigen did not bind these sequences present in single-stranded RNA. RNA/RNA duplexes, or RNA/DNA hybrids. T antigen did, however, bind as efficiently to single-stranded DNA-cellulose as to double-stranded DNA-cellulose. This binding was nonspecific because it was independent of the presence of T-antigen-binding sites. The implications of these observations are discussed.

Original languageEnglish (US)
Pages (from-to)2204-2208
Number of pages5
JournalJournal of Virology
Volume62
Issue number6
StatePublished - Jan 1 1988
Externally publishedYes

Fingerprint

Simian virus 40
regulatory sequences
single-stranded DNA
Single-Stranded DNA
Viral Tumor Antigens
RNA
antigens
DNA
Binding Sites
T Independent Antigens
binding sites
cellulose
Nucleic Acid Regulatory Sequences
Immunoassay
immunoassays

ASJC Scopus subject areas

  • Immunology
  • Medicine(all)
  • Virology

Cite this

Simian virus 40 (SV40) T antigen binds specifically to double-stranded DNA but not to single-stranded DNA or DNA/RNA hybrids containing the SV40 regulatory sequences. / Auborn, K. J.; Markowitz, Rhea-Beth; Wang, E.; Yu, Y. T.; Prives, C.

In: Journal of Virology, Vol. 62, No. 6, 01.01.1988, p. 2204-2208.

Research output: Contribution to journalArticle

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