Simultaneous cardiac mechanics and phosphorus‐31 NMR spectroscopy during myocardial ischemia and reperfusion in the intact dog

Donald D Miller, Felix Salinas, Richard A. Walsh

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

To investigate the high‐energy phosphate metabolic correlates of left ventricular (LV) dysfunction during the onset and recovery from severe, global myocardial ischemia in vivo, seven preinstrumented closed‐chest dogs had ECG‐gated phosphorus‐31 (31P) NMR‐spectroscopy (NMR‐S) studies performed and LV micromanometer and sonomicrometer data measured before, during, and every 5 min following severe occlusive global myocardial ischemia. Ischemic LV + dP/dtmax fell from 2396 ± 576 mm Hg/s at baseline to 2185 ± 478 mm Hg/s (p < 0.05) and did not normalize until after 30 min of reperfusion. LV ejection fraction (EF) decreased significantly (0.32 ± 0.07 EF units to 0.12 ± 0.13 EF units; P < 0.05) and did not recover by 30 min of reperfusion (0.27 ±0.09 units; P < 0.05 vs baseline). Simultaneous 31P NMR‐S studies demonstrated excellent β ‐ATP signal‐to‐noise (10 ± 4:1). Myocardial acidosis occurred during global ischemia (ApH = −0.22 ± 0.23 units; p < 0.05), with recovery at 30 min of reperfusion. Inorganic phosphate/ phosphocreatine ratio (Pi/PCr) increased significantly during ischemia (0.46 ± 0.07 to 0.61 ± 0.07; P < 0.05), with delayed normalization of this ratio at 30 min of reperfusion. β‐ATP peak area did not change during ischemia. Pi/PCr and LV contractility ( + dP/ dtmax ) were significantly correlated at baseline (r = −0.70) and during global ischemia (r = −0.78; p < 0.01), but not during recovery (r = 0.006; p = NS). Therefore, the simultaneous evaluation of high‐fidelity hemodynamic data and topical 31P NMR‐S can be performed in the intact State. © 1991 Academic Press, Inc.

Original languageEnglish (US)
Pages (from-to)41-52
Number of pages12
JournalMagnetic Resonance in Medicine
Volume17
Issue number1
DOIs
StatePublished - Jan 1 1991

Fingerprint

Myocardial Reperfusion
Mechanics
Reperfusion
Myocardial Ischemia
Magnetic Resonance Spectroscopy
Ischemia
Dogs
Adenosine Triphosphate
Phosphates
Phosphocreatine
Left Ventricular Dysfunction
Acidosis
Stroke Volume
Hemodynamics

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

Cite this

Simultaneous cardiac mechanics and phosphorus‐31 NMR spectroscopy during myocardial ischemia and reperfusion in the intact dog. / Miller, Donald D; Salinas, Felix; Walsh, Richard A.

In: Magnetic Resonance in Medicine, Vol. 17, No. 1, 01.01.1991, p. 41-52.

Research output: Contribution to journalArticle

@article{011691d7661447478cea1949bc1bed21,
title = "Simultaneous cardiac mechanics and phosphorus‐31 NMR spectroscopy during myocardial ischemia and reperfusion in the intact dog",
abstract = "To investigate the high‐energy phosphate metabolic correlates of left ventricular (LV) dysfunction during the onset and recovery from severe, global myocardial ischemia in vivo, seven preinstrumented closed‐chest dogs had ECG‐gated phosphorus‐31 (31P) NMR‐spectroscopy (NMR‐S) studies performed and LV micromanometer and sonomicrometer data measured before, during, and every 5 min following severe occlusive global myocardial ischemia. Ischemic LV + dP/dtmax fell from 2396 ± 576 mm Hg/s at baseline to 2185 ± 478 mm Hg/s (p < 0.05) and did not normalize until after 30 min of reperfusion. LV ejection fraction (EF) decreased significantly (0.32 ± 0.07 EF units to 0.12 ± 0.13 EF units; P < 0.05) and did not recover by 30 min of reperfusion (0.27 ±0.09 units; P < 0.05 vs baseline). Simultaneous 31P NMR‐S studies demonstrated excellent β ‐ATP signal‐to‐noise (10 ± 4:1). Myocardial acidosis occurred during global ischemia (ApH = −0.22 ± 0.23 units; p < 0.05), with recovery at 30 min of reperfusion. Inorganic phosphate/ phosphocreatine ratio (Pi/PCr) increased significantly during ischemia (0.46 ± 0.07 to 0.61 ± 0.07; P < 0.05), with delayed normalization of this ratio at 30 min of reperfusion. β‐ATP peak area did not change during ischemia. Pi/PCr and LV contractility ( + dP/ dtmax ) were significantly correlated at baseline (r = −0.70) and during global ischemia (r = −0.78; p < 0.01), but not during recovery (r = 0.006; p = NS). Therefore, the simultaneous evaluation of high‐fidelity hemodynamic data and topical 31P NMR‐S can be performed in the intact State. {\circledC} 1991 Academic Press, Inc.",
author = "Miller, {Donald D} and Felix Salinas and Walsh, {Richard A.}",
year = "1991",
month = "1",
day = "1",
doi = "10.1002/mrm.1910170109",
language = "English (US)",
volume = "17",
pages = "41--52",
journal = "Magnetic Resonance in Medicine",
issn = "0740-3194",
publisher = "John Wiley and Sons Inc.",
number = "1",

}

TY - JOUR

T1 - Simultaneous cardiac mechanics and phosphorus‐31 NMR spectroscopy during myocardial ischemia and reperfusion in the intact dog

AU - Miller, Donald D

AU - Salinas, Felix

AU - Walsh, Richard A.

PY - 1991/1/1

Y1 - 1991/1/1

N2 - To investigate the high‐energy phosphate metabolic correlates of left ventricular (LV) dysfunction during the onset and recovery from severe, global myocardial ischemia in vivo, seven preinstrumented closed‐chest dogs had ECG‐gated phosphorus‐31 (31P) NMR‐spectroscopy (NMR‐S) studies performed and LV micromanometer and sonomicrometer data measured before, during, and every 5 min following severe occlusive global myocardial ischemia. Ischemic LV + dP/dtmax fell from 2396 ± 576 mm Hg/s at baseline to 2185 ± 478 mm Hg/s (p < 0.05) and did not normalize until after 30 min of reperfusion. LV ejection fraction (EF) decreased significantly (0.32 ± 0.07 EF units to 0.12 ± 0.13 EF units; P < 0.05) and did not recover by 30 min of reperfusion (0.27 ±0.09 units; P < 0.05 vs baseline). Simultaneous 31P NMR‐S studies demonstrated excellent β ‐ATP signal‐to‐noise (10 ± 4:1). Myocardial acidosis occurred during global ischemia (ApH = −0.22 ± 0.23 units; p < 0.05), with recovery at 30 min of reperfusion. Inorganic phosphate/ phosphocreatine ratio (Pi/PCr) increased significantly during ischemia (0.46 ± 0.07 to 0.61 ± 0.07; P < 0.05), with delayed normalization of this ratio at 30 min of reperfusion. β‐ATP peak area did not change during ischemia. Pi/PCr and LV contractility ( + dP/ dtmax ) were significantly correlated at baseline (r = −0.70) and during global ischemia (r = −0.78; p < 0.01), but not during recovery (r = 0.006; p = NS). Therefore, the simultaneous evaluation of high‐fidelity hemodynamic data and topical 31P NMR‐S can be performed in the intact State. © 1991 Academic Press, Inc.

AB - To investigate the high‐energy phosphate metabolic correlates of left ventricular (LV) dysfunction during the onset and recovery from severe, global myocardial ischemia in vivo, seven preinstrumented closed‐chest dogs had ECG‐gated phosphorus‐31 (31P) NMR‐spectroscopy (NMR‐S) studies performed and LV micromanometer and sonomicrometer data measured before, during, and every 5 min following severe occlusive global myocardial ischemia. Ischemic LV + dP/dtmax fell from 2396 ± 576 mm Hg/s at baseline to 2185 ± 478 mm Hg/s (p < 0.05) and did not normalize until after 30 min of reperfusion. LV ejection fraction (EF) decreased significantly (0.32 ± 0.07 EF units to 0.12 ± 0.13 EF units; P < 0.05) and did not recover by 30 min of reperfusion (0.27 ±0.09 units; P < 0.05 vs baseline). Simultaneous 31P NMR‐S studies demonstrated excellent β ‐ATP signal‐to‐noise (10 ± 4:1). Myocardial acidosis occurred during global ischemia (ApH = −0.22 ± 0.23 units; p < 0.05), with recovery at 30 min of reperfusion. Inorganic phosphate/ phosphocreatine ratio (Pi/PCr) increased significantly during ischemia (0.46 ± 0.07 to 0.61 ± 0.07; P < 0.05), with delayed normalization of this ratio at 30 min of reperfusion. β‐ATP peak area did not change during ischemia. Pi/PCr and LV contractility ( + dP/ dtmax ) were significantly correlated at baseline (r = −0.70) and during global ischemia (r = −0.78; p < 0.01), but not during recovery (r = 0.006; p = NS). Therefore, the simultaneous evaluation of high‐fidelity hemodynamic data and topical 31P NMR‐S can be performed in the intact State. © 1991 Academic Press, Inc.

UR - http://www.scopus.com/inward/record.url?scp=0026008132&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026008132&partnerID=8YFLogxK

U2 - 10.1002/mrm.1910170109

DO - 10.1002/mrm.1910170109

M3 - Article

C2 - 2067406

AN - SCOPUS:0026008132

VL - 17

SP - 41

EP - 52

JO - Magnetic Resonance in Medicine

JF - Magnetic Resonance in Medicine

SN - 0740-3194

IS - 1

ER -