Simultaneous determination of tianeptine and its active metabolite tianeptine MC5 in rat plasma and brain tissue using high performance liquid chromatography/electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS)

N. M. El Zahar, N. Magdy, A. M. El-Kosasy, Wayne D. Beck, Patrick M. Callahan, Alvin V. Terry, Michael G. Bartlett

Research output: Contribution to journalArticle


A selective and sensitive method was developed to simultaneously quantify tianeptine and its main active metabolite tianeptine MC5 in rat plasma and brain tissue. A one-step liquid-liquid extraction was developed for sample preparation. The linear range for plasma was from 1.0-500.0 ng mL-1 and for brain tissue it was from 1.0-500.0 ng g-1. The method was validated in accordance with US FDA guidelines (precision within 15% relative standard deviation (RSD) and accuracy within 15% relative error (RE) with a consistent recovery). Stable isotope-labeled tianeptine and tianeptine MC5 (tianeptine-D4 and tianeptine MC5-D4) were used as the internal standards achieving good reproducibility while reducing the matrix effects. This method was successfully applied to a preclinical study for the simultaneous determination of tianeptine and tianeptine MC5 in rat plasma and brain tissue.

Original languageEnglish (US)
Pages (from-to)439-447
Number of pages9
JournalAnalytical Methods
Issue number4
Publication statusPublished - Jan 28 2018


ASJC Scopus subject areas

  • Analytical Chemistry
  • Chemical Engineering(all)
  • Engineering(all)

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