Simultaneous quantitation of quetiapine and its active metabolite norquetiapine in rat plasma and brain tissue by high performance liquid chromatography/electrospray ionization tandem mass spectrometry (LC-MS/MS)

Xiangkun Yang; Indrani Poddar; Caterina M. Hernandez; Alvin V Terry; Michael G. Bartlett

doi:10.1016/j.jchromb.2015.08.011

Simultaneous quantitation of quetiapine and its active metabolite norquetiapine in rat plasma and brain tissue by high performance liquid chromatography/electrospray ionization tandem mass spectrometry (LC-MS/MS)

Xiangkun Yang, Indrani Poddar, Caterina M. Hernandez, Alvin V Terry, Michael G. Bartlett

Pharmacology and Toxicology

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

A sensitive method to simultaneously quantitate quetiapine and norquetiapine in rat plasma and brain tissue was developed using a one-step liquid-liquid extraction for sample preparation and LC-MS/MS for detection. The method provided a linear range of 1.0-500.0. ng/mL for each analyte in plasma and 3.0-1500.0. ng/g in brain tissue. The method was validated with precision within 15% relative standard deviation (RSD), accuracy within 15% relative error (RE), matrix effects within 10% and a consistent recovery. This method has been successfully applied in a preclinical study of quetiapine and norquetiapine to simultaneously determine their concentrations in rat plasma and brain tissue.

Original language	English (US)
Pages (from-to)	71-77
Number of pages	7
Journal	Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume	1002
DOIs	https://doi.org/10.1016/j.jchromb.2015.08.011
State	Published - Oct 1 2015

Keywords

Brain
LC-MS/MS
Norquetiapine
Plasma
Quetiapine

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Clinical Biochemistry
Cell Biology

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Yang, X., Poddar, I., Hernandez, C. M., Terry, A. V., & Bartlett, M. G. (2015). Simultaneous quantitation of quetiapine and its active metabolite norquetiapine in rat plasma and brain tissue by high performance liquid chromatography/electrospray ionization tandem mass spectrometry (LC-MS/MS). Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 1002, 71-77. https://doi.org/10.1016/j.jchromb.2015.08.011

Simultaneous quantitation of quetiapine and its active metabolite norquetiapine in rat plasma and brain tissue by high performance liquid chromatography/electrospray ionization tandem mass spectrometry (LC-MS/MS). / Yang, Xiangkun; Poddar, Indrani; Hernandez, Caterina M. et al.
In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, Vol. 1002, 01.10.2015, p. 71-77.

Research output: Contribution to journal › Article › peer-review

Yang, X, Poddar, I, Hernandez, CM, Terry, AV & Bartlett, MG 2015, 'Simultaneous quantitation of quetiapine and its active metabolite norquetiapine in rat plasma and brain tissue by high performance liquid chromatography/electrospray ionization tandem mass spectrometry (LC-MS/MS)', Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, vol. 1002, pp. 71-77. https://doi.org/10.1016/j.jchromb.2015.08.011

Yang X, Poddar I, Hernandez CM, Terry AV, Bartlett MG. Simultaneous quantitation of quetiapine and its active metabolite norquetiapine in rat plasma and brain tissue by high performance liquid chromatography/electrospray ionization tandem mass spectrometry (LC-MS/MS). Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences. 2015 Oct 1;1002:71-77. doi: 10.1016/j.jchromb.2015.08.011

Yang, Xiangkun ; Poddar, Indrani ; Hernandez, Caterina M. et al. / Simultaneous quantitation of quetiapine and its active metabolite norquetiapine in rat plasma and brain tissue by high performance liquid chromatography/electrospray ionization tandem mass spectrometry (LC-MS/MS). In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences. 2015 ; Vol. 1002. pp. 71-77.

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Simultaneous quantitation of quetiapine and its active metabolite norquetiapine in rat plasma and brain tissue by high performance liquid chromatography/electrospray ionization tandem mass spectrometry (LC-MS/MS)

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

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