Simultaneous quantitation of quetiapine and its active metabolite norquetiapine in rat plasma and brain tissue by high performance liquid chromatography/electrospray ionization tandem mass spectrometry (LC-MS/MS)

Xiangkun Yang, Indrani Poddar, Caterina M. Hernandez, Alvin V Terry, Michael G. Bartlett

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

A sensitive method to simultaneously quantitate quetiapine and norquetiapine in rat plasma and brain tissue was developed using a one-step liquid-liquid extraction for sample preparation and LC-MS/MS for detection. The method provided a linear range of 1.0-500.0. ng/mL for each analyte in plasma and 3.0-1500.0. ng/g in brain tissue. The method was validated with precision within 15% relative standard deviation (RSD), accuracy within 15% relative error (RE), matrix effects within 10% and a consistent recovery. This method has been successfully applied in a preclinical study of quetiapine and norquetiapine to simultaneously determine their concentrations in rat plasma and brain tissue.

Original languageEnglish (US)
Pages (from-to)71-77
Number of pages7
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume1002
DOIs
StatePublished - Oct 1 2015

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Electrospray ionization
Electrospray Ionization Mass Spectrometry
High performance liquid chromatography
Metabolites
Tandem Mass Spectrometry
Mass spectrometry
Rats
Brain
High Pressure Liquid Chromatography
Tissue
Plasmas
Liquids
Liquid-Liquid Extraction
Recovery
norquetiapine
Quetiapine Fumarate

Keywords

  • Brain
  • LC-MS/MS
  • Norquetiapine
  • Plasma
  • Quetiapine

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Cell Biology

Cite this

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title = "Simultaneous quantitation of quetiapine and its active metabolite norquetiapine in rat plasma and brain tissue by high performance liquid chromatography/electrospray ionization tandem mass spectrometry (LC-MS/MS)",
abstract = "A sensitive method to simultaneously quantitate quetiapine and norquetiapine in rat plasma and brain tissue was developed using a one-step liquid-liquid extraction for sample preparation and LC-MS/MS for detection. The method provided a linear range of 1.0-500.0. ng/mL for each analyte in plasma and 3.0-1500.0. ng/g in brain tissue. The method was validated with precision within 15{\%} relative standard deviation (RSD), accuracy within 15{\%} relative error (RE), matrix effects within 10{\%} and a consistent recovery. This method has been successfully applied in a preclinical study of quetiapine and norquetiapine to simultaneously determine their concentrations in rat plasma and brain tissue.",
keywords = "Brain, LC-MS/MS, Norquetiapine, Plasma, Quetiapine",
author = "Xiangkun Yang and Indrani Poddar and Hernandez, {Caterina M.} and Terry, {Alvin V} and Bartlett, {Michael G.}",
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T1 - Simultaneous quantitation of quetiapine and its active metabolite norquetiapine in rat plasma and brain tissue by high performance liquid chromatography/electrospray ionization tandem mass spectrometry (LC-MS/MS)

AU - Yang, Xiangkun

AU - Poddar, Indrani

AU - Hernandez, Caterina M.

AU - Terry, Alvin V

AU - Bartlett, Michael G.

PY - 2015/10/1

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N2 - A sensitive method to simultaneously quantitate quetiapine and norquetiapine in rat plasma and brain tissue was developed using a one-step liquid-liquid extraction for sample preparation and LC-MS/MS for detection. The method provided a linear range of 1.0-500.0. ng/mL for each analyte in plasma and 3.0-1500.0. ng/g in brain tissue. The method was validated with precision within 15% relative standard deviation (RSD), accuracy within 15% relative error (RE), matrix effects within 10% and a consistent recovery. This method has been successfully applied in a preclinical study of quetiapine and norquetiapine to simultaneously determine their concentrations in rat plasma and brain tissue.

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