Simultaneous quantitation of quetiapine and its active metabolite norquetiapine in rat plasma and brain tissue by high performance liquid chromatography/electrospray ionization tandem mass spectrometry (LC-MS/MS)

Xiangkun Yang, Indrani Poddar, Caterina M. Hernandez, Alvin V Terry, Michael G. Bartlett

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

A sensitive method to simultaneously quantitate quetiapine and norquetiapine in rat plasma and brain tissue was developed using a one-step liquid-liquid extraction for sample preparation and LC-MS/MS for detection. The method provided a linear range of 1.0-500.0. ng/mL for each analyte in plasma and 3.0-1500.0. ng/g in brain tissue. The method was validated with precision within 15% relative standard deviation (RSD), accuracy within 15% relative error (RE), matrix effects within 10% and a consistent recovery. This method has been successfully applied in a preclinical study of quetiapine and norquetiapine to simultaneously determine their concentrations in rat plasma and brain tissue.

Original languageEnglish (US)
Pages (from-to)71-77
Number of pages7
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume1002
DOIs
StatePublished - Oct 1 2015

Keywords

  • Brain
  • LC-MS/MS
  • Norquetiapine
  • Plasma
  • Quetiapine

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Cell Biology

Fingerprint Dive into the research topics of 'Simultaneous quantitation of quetiapine and its active metabolite norquetiapine in rat plasma and brain tissue by high performance liquid chromatography/electrospray ionization tandem mass spectrometry (LC-MS/MS)'. Together they form a unique fingerprint.

  • Cite this