Abstract
A sensitive method to simultaneously quantitate quetiapine and norquetiapine in rat plasma and brain tissue was developed using a one-step liquid-liquid extraction for sample preparation and LC-MS/MS for detection. The method provided a linear range of 1.0-500.0. ng/mL for each analyte in plasma and 3.0-1500.0. ng/g in brain tissue. The method was validated with precision within 15% relative standard deviation (RSD), accuracy within 15% relative error (RE), matrix effects within 10% and a consistent recovery. This method has been successfully applied in a preclinical study of quetiapine and norquetiapine to simultaneously determine their concentrations in rat plasma and brain tissue.
Original language | English (US) |
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Pages (from-to) | 71-77 |
Number of pages | 7 |
Journal | Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences |
Volume | 1002 |
DOIs | |
State | Published - Oct 1 2015 |
Keywords
- Brain
- LC-MS/MS
- Norquetiapine
- Plasma
- Quetiapine
ASJC Scopus subject areas
- Analytical Chemistry
- Biochemistry
- Clinical Biochemistry
- Cell Biology