Simultaneous recording of the field-EPSP as well as the population spike in the CA1 region in freely moving rats by using a fixed "double" -recording electrode

Thomas Scherf, Julietta Uta Frey, Sabine Martina Frey

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The recording of field potentials in freely moving rats is a very appropriate and commonly used method to describe changes in cellular mechanisms underlying synaptic plasticity. Recently, we introduced a method for the simultaneous recording of both the field-EPSP as well as the population spike in the dentate gyrus of freely moving rats. We used self-made " double" -recording electrodes, consisting of two wires straighten together with a constant distance between both tips. This method was now further developed to obtain stable long-term recordings of CA1 field potentials. Rats were chronically implanted with a bipolar recording electrode; one tip of which reached the stratum radiatum to record the field-EPSP, the other tip was lowered into the stratum pyramidale of the same neuron population to record the population spike by stimulation of the contralateral CA3 (cCA3). In such prepared rats, simultaneously recorded field-EPSP as well as the population spike where thus obtained from their places of generation in a very reliable manner. This kind of preparation allowed a better standardization of stimulation intensities between different animals and stable electrophysiological recordings of both CA1-potentials over a time period of at least 24. h in freely behaving animals. Furthermore, primed burst stimulation of the cCA3 (a single biphasic priming pulse was followed by a burst of 10 pulses (frequency of 100. Hz) 190. ms later; pulse duration per half-wave: 0.1. ms) resulted in an early-LTP of both measured parameters, the field-EPSP and the population spike in the CA1 region of freely moving rats.

Original languageEnglish (US)
Pages (from-to)1-6
Number of pages6
JournalJournal of Neuroscience Methods
Volume188
Issue number1
DOIs
StatePublished - Apr 1 2010

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Excitatory Postsynaptic Potentials
Electrodes
Population
Neuronal Plasticity
Dentate Gyrus
Neurons

Keywords

  • "Double" -recording
  • Contralateral CA3
  • Electrophysiological recordings
  • Field-EPSP
  • Freely moving rats
  • Hippocampal CA1
  • Population spike

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Simultaneous recording of the field-EPSP as well as the population spike in the CA1 region in freely moving rats by using a fixed "double" -recording electrode. / Scherf, Thomas; Frey, Julietta Uta; Frey, Sabine Martina.

In: Journal of Neuroscience Methods, Vol. 188, No. 1, 01.04.2010, p. 1-6.

Research output: Contribution to journalArticle

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