Single slice method for quantification of hemorrhagic transformation using direct ELISA

Hend M. Hilali, Alexis N. Simpkins, William D. Hill, Jennifer L. Waller, Robert A. Knight, Susan C. Fagan

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

An important area of experimental stroke research addresses the development of brain hemorrhage after cerebral ischemia. Investigations of hemorrhagic transformation, however, have been compromised by the absence of sensitive methods for quantification of hemorrhagic transformation. We have developed a direct ELISA method that is sensitive, reproducible and can be conducted on tissue treated with triphenyltetrazolium chloride, a stain commonly used for infarct size determination. We have also localized the slices containing the most hemoglobin to facilitate a single slice analysis. This allows two essential variables in experimental stroke research to be assessed on the same slice, leaving the rest of the brain available for other analysis.

Original languageEnglish (US)
Pages (from-to)93-98
Number of pages6
JournalNeurological Research
Volume26
Issue number1
DOIs
StatePublished - Jan 1 2004

Keywords

  • ELISA
  • Hemoglobin
  • Hemorrhagic transformation
  • Rats
  • Stroke

ASJC Scopus subject areas

  • Neurology
  • Clinical Neurology

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