Sinus augmentation using a mini-pig model: Effect of ceramic and allogeneic bone biomaterials

Cristiano Susin, Tiago Fiorini, Jaebum Lee, Rubens Moreno de Freitas, Hsien Chung Chiu, Hari S. Prasad, Amanda N. Buxton, Ulf M.E. Wikesjö

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Background: Present clinical practice broadly relies on off-the-shelf allogeneic, xenogeneic or synthetic bone biomaterials in support of sinus augmentation. Also, recombinant human bone morphogenetic protein-2 in an absorbable collagen sponge carrier (rhBMP-2/ACS) has been shown to support clinically relevant bone formation when used to augment the maxillary sinus. Objectives: To evaluate local bone formation/dental implant osseointegration following implantation of two particulate bone biomaterials using the mini-pig sinus augmentation model. Methods: Nine adult Göttingen mini-pigs were used for evaluation of a biphasic ceramic (15%/85% HA/ß-TCP) and an allogeneic mineralized bone biomaterial. Treatments randomized to contralateral sinus sites included sham-surgery (control) and biomaterials. Two threaded dental implants (ø4.0 × 11.5 mm) were placed at each sinus site. The animals were euthanized at 8 weeks for histologic analysis. Results: Execution of the surgical protocol and healing was unremarkable. Limited infraorbital swelling was observed until suture removal. The biphasic ceramic and allogeneic bone biomaterials produced significantly increased bone formation (5.2 ± 1.9 mm and 4.9 ± 1.6 mm vs. 2.6 ± 0.5 mm, p < 0.05) and osseointegration (18.0 ± 6.0% and 25.1 ± 18.2% vs. 10.1 ± 8.0%, p < 0.05) over the sham-surgery control. No significant differences were observed between biomaterials. Conclusions: Implantation of biphasic ceramic or allogeneic bone biomaterials enhances bone formation in the mini-pig maxillary sinus, however, dental implant bone support is incomplete resulting in overall limited osseointegration.

Original languageEnglish (US)
Pages (from-to)1059-1066
Number of pages8
JournalJournal of Clinical Periodontology
Volume44
Issue number10
DOIs
StatePublished - Oct 1 2017

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Ceramics
Biocompatible Materials
Swine
Bone and Bones
Osseointegration
Osteogenesis
Dental Implants
Maxillary Sinus
Porifera
Sutures
Collagen

Keywords

  • bone biomaterials
  • bone morphogenetic protein-2
  • mini-pig model
  • osseointegration
  • sinus augmentation

ASJC Scopus subject areas

  • Periodontics

Cite this

Susin, C., Fiorini, T., Lee, J., de Freitas, R. M., Chiu, H. C., Prasad, H. S., ... Wikesjö, U. M. E. (2017). Sinus augmentation using a mini-pig model: Effect of ceramic and allogeneic bone biomaterials. Journal of Clinical Periodontology, 44(10), 1059-1066. https://doi.org/10.1111/jcpe.12766

Sinus augmentation using a mini-pig model : Effect of ceramic and allogeneic bone biomaterials. / Susin, Cristiano; Fiorini, Tiago; Lee, Jaebum; de Freitas, Rubens Moreno; Chiu, Hsien Chung; Prasad, Hari S.; Buxton, Amanda N.; Wikesjö, Ulf M.E.

In: Journal of Clinical Periodontology, Vol. 44, No. 10, 01.10.2017, p. 1059-1066.

Research output: Contribution to journalArticle

Susin, C, Fiorini, T, Lee, J, de Freitas, RM, Chiu, HC, Prasad, HS, Buxton, AN & Wikesjö, UME 2017, 'Sinus augmentation using a mini-pig model: Effect of ceramic and allogeneic bone biomaterials', Journal of Clinical Periodontology, vol. 44, no. 10, pp. 1059-1066. https://doi.org/10.1111/jcpe.12766
Susin, Cristiano ; Fiorini, Tiago ; Lee, Jaebum ; de Freitas, Rubens Moreno ; Chiu, Hsien Chung ; Prasad, Hari S. ; Buxton, Amanda N. ; Wikesjö, Ulf M.E. / Sinus augmentation using a mini-pig model : Effect of ceramic and allogeneic bone biomaterials. In: Journal of Clinical Periodontology. 2017 ; Vol. 44, No. 10. pp. 1059-1066.
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abstract = "Background: Present clinical practice broadly relies on off-the-shelf allogeneic, xenogeneic or synthetic bone biomaterials in support of sinus augmentation. Also, recombinant human bone morphogenetic protein-2 in an absorbable collagen sponge carrier (rhBMP-2/ACS) has been shown to support clinically relevant bone formation when used to augment the maxillary sinus. Objectives: To evaluate local bone formation/dental implant osseointegration following implantation of two particulate bone biomaterials using the mini-pig sinus augmentation model. Methods: Nine adult G{\"o}ttingen mini-pigs were used for evaluation of a biphasic ceramic (15{\%}/85{\%} HA/{\ss}-TCP) and an allogeneic mineralized bone biomaterial. Treatments randomized to contralateral sinus sites included sham-surgery (control) and biomaterials. Two threaded dental implants ({\o}4.0 × 11.5 mm) were placed at each sinus site. The animals were euthanized at 8 weeks for histologic analysis. Results: Execution of the surgical protocol and healing was unremarkable. Limited infraorbital swelling was observed until suture removal. The biphasic ceramic and allogeneic bone biomaterials produced significantly increased bone formation (5.2 ± 1.9 mm and 4.9 ± 1.6 mm vs. 2.6 ± 0.5 mm, p < 0.05) and osseointegration (18.0 ± 6.0{\%} and 25.1 ± 18.2{\%} vs. 10.1 ± 8.0{\%}, p < 0.05) over the sham-surgery control. No significant differences were observed between biomaterials. Conclusions: Implantation of biphasic ceramic or allogeneic bone biomaterials enhances bone formation in the mini-pig maxillary sinus, however, dental implant bone support is incomplete resulting in overall limited osseointegration.",
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AU - Fiorini, Tiago

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AU - de Freitas, Rubens Moreno

AU - Chiu, Hsien Chung

AU - Prasad, Hari S.

AU - Buxton, Amanda N.

AU - Wikesjö, Ulf M.E.

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