Six-month evaluation of adhesives interface created by a hydrophobic adhesive to acid-etched ethanol-wet bonded dentine with simplified dehydration protocols

Fernanda T. Sadek, Annalisa Mazzoni, Lorenzo Breschi, Franklin Chi Meng Tay, Roberto R. Braga

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Objectives: To evaluate the efficacy of simplified dehydration protocols, in the absence of tubular occlusion, on bond strength and interfacial nanoleakage of a hydrophobic experimental adhesive blend to acid-etched, ethanol-dehydrated dentine immediately and after 6 months. Methods: Molars were randomly assigned to 6 treatment groups (n = 5). Under pulpal pressure simulation, dentine crowns were acid-etched with 35% H3PO4 and rinsed with water. Adper Scotchbond Multi-Purpose was used for the control group. The remaining groups had their dentine surface dehydrated with ethanol solutions: group 1 = 50%, 70%, 80%, 95% and 3 × 100%, 30 s for each application; group 2 the same ethanol sequence with 15 s for each solution; groups 3, 4 and 5 used 100% ethanol only, applied in seven, three or one 30 s step, respectively. After dehydration, a primer (50% BisGMA + TEGDMA, 50% ethanol) was used, followed by the neat comonomer adhesive application. Resin composite build-ups were then prepared using an incremental technique. Specimens were stored for 24 h, sectioned into beams and stressed to failure after 24 h or after 6 months of artificial ageing. Interfacial silver leakage evaluation was performed for both storage periods (n = 5 per subgroup). Results: Group 1 showed higher bond strengths at 24 h or after 6 months of ageing (45.6 ± 5.9a/43.1 ± 3.2a MPa) and lower silver impregnation. Bond strength results were statistically similar to control group (41.2 ± 3.3ab/38.3 ± 4.0ab MPa), group 2 (40.0 ± 3.1ab/38.6 ± 3.2ab MPa), and group 3 at 24 h (35.5 ± 4.3ab MPa). Groups 4 (34.6 ± 5.7bc/25.9 ± 4.1c MPa) and 5 (24.7 ± 4.9c/18.2 ± 4.2c MPa) resulted in lower bond strengths, extensive interfacial nanoleakage and more prominent reductions (up to 25%) in bond strengths after 6 months of ageing. Conclusions: Simplified dehydration protocols using one or three 100% ethanol applications should be avoided for the ethanol-wet bonding technique in the absence of tubular occlusion, as they showed decreased bond strength, more severe nanoleakage and reduced bond stability over time.

Original languageEnglish (US)
Pages (from-to)276-283
Number of pages8
JournalJournal of Dentistry
Volume38
Issue number4
DOIs
StatePublished - Apr 1 2010

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Dentin
Dehydration
Adhesives
Ethanol
Acids
Silver
Control Groups
Composite Resins
Crowns
Pressure
Water

Keywords

  • Bond strength
  • Dentine
  • Ethanol-wet bonding
  • Interfacial nanoleakage

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Six-month evaluation of adhesives interface created by a hydrophobic adhesive to acid-etched ethanol-wet bonded dentine with simplified dehydration protocols. / Sadek, Fernanda T.; Mazzoni, Annalisa; Breschi, Lorenzo; Tay, Franklin Chi Meng; Braga, Roberto R.

In: Journal of Dentistry, Vol. 38, No. 4, 01.04.2010, p. 276-283.

Research output: Contribution to journalArticle

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abstract = "Objectives: To evaluate the efficacy of simplified dehydration protocols, in the absence of tubular occlusion, on bond strength and interfacial nanoleakage of a hydrophobic experimental adhesive blend to acid-etched, ethanol-dehydrated dentine immediately and after 6 months. Methods: Molars were randomly assigned to 6 treatment groups (n = 5). Under pulpal pressure simulation, dentine crowns were acid-etched with 35{\%} H3PO4 and rinsed with water. Adper Scotchbond Multi-Purpose was used for the control group. The remaining groups had their dentine surface dehydrated with ethanol solutions: group 1 = 50{\%}, 70{\%}, 80{\%}, 95{\%} and 3 × 100{\%}, 30 s for each application; group 2 the same ethanol sequence with 15 s for each solution; groups 3, 4 and 5 used 100{\%} ethanol only, applied in seven, three or one 30 s step, respectively. After dehydration, a primer (50{\%} BisGMA + TEGDMA, 50{\%} ethanol) was used, followed by the neat comonomer adhesive application. Resin composite build-ups were then prepared using an incremental technique. Specimens were stored for 24 h, sectioned into beams and stressed to failure after 24 h or after 6 months of artificial ageing. Interfacial silver leakage evaluation was performed for both storage periods (n = 5 per subgroup). Results: Group 1 showed higher bond strengths at 24 h or after 6 months of ageing (45.6 ± 5.9a/43.1 ± 3.2a MPa) and lower silver impregnation. Bond strength results were statistically similar to control group (41.2 ± 3.3ab/38.3 ± 4.0ab MPa), group 2 (40.0 ± 3.1ab/38.6 ± 3.2ab MPa), and group 3 at 24 h (35.5 ± 4.3ab MPa). Groups 4 (34.6 ± 5.7bc/25.9 ± 4.1c MPa) and 5 (24.7 ± 4.9c/18.2 ± 4.2c MPa) resulted in lower bond strengths, extensive interfacial nanoleakage and more prominent reductions (up to 25{\%}) in bond strengths after 6 months of ageing. Conclusions: Simplified dehydration protocols using one or three 100{\%} ethanol applications should be avoided for the ethanol-wet bonding technique in the absence of tubular occlusion, as they showed decreased bond strength, more severe nanoleakage and reduced bond stability over time.",
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AU - Sadek, Fernanda T.

AU - Mazzoni, Annalisa

AU - Breschi, Lorenzo

AU - Tay, Franklin Chi Meng

AU - Braga, Roberto R.

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N2 - Objectives: To evaluate the efficacy of simplified dehydration protocols, in the absence of tubular occlusion, on bond strength and interfacial nanoleakage of a hydrophobic experimental adhesive blend to acid-etched, ethanol-dehydrated dentine immediately and after 6 months. Methods: Molars were randomly assigned to 6 treatment groups (n = 5). Under pulpal pressure simulation, dentine crowns were acid-etched with 35% H3PO4 and rinsed with water. Adper Scotchbond Multi-Purpose was used for the control group. The remaining groups had their dentine surface dehydrated with ethanol solutions: group 1 = 50%, 70%, 80%, 95% and 3 × 100%, 30 s for each application; group 2 the same ethanol sequence with 15 s for each solution; groups 3, 4 and 5 used 100% ethanol only, applied in seven, three or one 30 s step, respectively. After dehydration, a primer (50% BisGMA + TEGDMA, 50% ethanol) was used, followed by the neat comonomer adhesive application. Resin composite build-ups were then prepared using an incremental technique. Specimens were stored for 24 h, sectioned into beams and stressed to failure after 24 h or after 6 months of artificial ageing. Interfacial silver leakage evaluation was performed for both storage periods (n = 5 per subgroup). Results: Group 1 showed higher bond strengths at 24 h or after 6 months of ageing (45.6 ± 5.9a/43.1 ± 3.2a MPa) and lower silver impregnation. Bond strength results were statistically similar to control group (41.2 ± 3.3ab/38.3 ± 4.0ab MPa), group 2 (40.0 ± 3.1ab/38.6 ± 3.2ab MPa), and group 3 at 24 h (35.5 ± 4.3ab MPa). Groups 4 (34.6 ± 5.7bc/25.9 ± 4.1c MPa) and 5 (24.7 ± 4.9c/18.2 ± 4.2c MPa) resulted in lower bond strengths, extensive interfacial nanoleakage and more prominent reductions (up to 25%) in bond strengths after 6 months of ageing. Conclusions: Simplified dehydration protocols using one or three 100% ethanol applications should be avoided for the ethanol-wet bonding technique in the absence of tubular occlusion, as they showed decreased bond strength, more severe nanoleakage and reduced bond stability over time.

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KW - Interfacial nanoleakage

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