Skizzle is a novel plasminogen- and plasmin-binding protein from Streptococcus agalactiae that targets proteins of human fibrinolysis to promote plasmin generation

Karen G. Wiles, Peter Panizzi, Heather K. Kroh, Paul E. Bock

Research output: Contribution to journalArticle

Abstract

Skizzle (SkzL), secreted by Streptococcus agalactiae, has moderate sequence identity to streptokinase and staphylokinase, bacterial activators of human plasminogen (Pg). SkzL binds [Glu]Pg with low affinity (KD3-16 μM) and [Lys]Pg and plasmin (Pm) with indistinguishable high affinity (K D 80 and 50 nM, respectively). Binding of SkzL to Pg and Pmis completely lysine-binding site-dependent, as shown by the effect of the lysine analog, 6-aminohexanoic acid. Deletion of the COOH-terminal SkzL Lys 415 residue reduces affinity for [Lys]Pg and active site-blocked Pm 30-fold, implicating Lys415 in a lysine-binding site interaction with a Pg/Pm kringle. SkzL binding to active site fluorescein-labeled Pg/Pm analogs demonstrates distinct high and low affinity interactions. High affinity binding is mediated by Lys415, whereas the source of low affinity binding is unknown. SkzL enhances the activation of [Glu]Pg by urokinase (uPA) ∼20-fold, to a maximum rate indistinguishable from that for [Lys]Pg and [Glu]Pg activation in the presence of 6-aminohexanoic acid. SkzL binds preferentially to the partially extended β-conformation of [Glu]Pg, which is in unfavorable equilibrium with the compact α-conformation, thereby converting [Glu]Pg to the fully extended γ-conformation and accelerating the rate of its activation by uPA. SkzL enhances [Lys]Pg and [Glu]Pg activation by single-chain tissue-type Pg activator, ∼42- and ∼650-fold, respectively. SkzL increases the rate of plasma clot lysis by uPA and single-chain tissue-type Pg activator ∼2-fold, confirming its cofactor activity in a physiological model system. The results suggest a role for SkzL in S. agalactiae pathogenesis through fibrinolytic enhancement.

Original languageEnglish (US)
Pages (from-to)21153-21164
Number of pages12
JournalJournal of Biological Chemistry
Volume285
Issue number27
DOIs
StatePublished - Jul 2 2010
Externally publishedYes

Fingerprint

Streptococcus agalactiae
Plasminogen
Fibrinolysin
Fibrinolysis
Carrier Proteins
Proteins
Lysine
Chemical activation
Aminocaproic Acid
Conformations
Tissue Plasminogen Activator
Catalytic Domain
Binding Sites
Kringles
Physiological models
Streptokinase
Plasminogen Activators
Urokinase-Type Plasminogen Activator
Fluorescein
Plasmas

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Skizzle is a novel plasminogen- and plasmin-binding protein from Streptococcus agalactiae that targets proteins of human fibrinolysis to promote plasmin generation. / Wiles, Karen G.; Panizzi, Peter; Kroh, Heather K.; Bock, Paul E.

In: Journal of Biological Chemistry, Vol. 285, No. 27, 02.07.2010, p. 21153-21164.

Research output: Contribution to journalArticle

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T1 - Skizzle is a novel plasminogen- and plasmin-binding protein from Streptococcus agalactiae that targets proteins of human fibrinolysis to promote plasmin generation

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